Registration Dossier

Diss Factsheets

Administrative data

Endpoint:
biochemical or cellular interactions
Type of information:
migrated information: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
supporting study
Study period:
1984
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference
Reference Type:
publication
Title:
Effects of cyclic 12-, 8- and 6-carbon compounds on glutathione S-transferase activity
Author:
Sparnins VL, Lam LKT and Wattenberg LW
Year:
1984
Bibliographic source:
Biochem. Biophys. Res. Commun. 120, 2, 637-640

Materials and methods

Principles of method if other than guideline:
Glutathione S-transferase is considered as a major detoxification system  which catalyzes conjugation of electrophilic compounds, e.g. chemical  
carcinogens, to glutathione. The effects of cyclic 12-, 8- and 6-carbon  compounds (incl. cyclododecanol) on the glutathione S-transferase 
(E.C  2.5.1.18) activity in the liver, intestinal mucosa and the forestomach of  female ICR/Ha mice were investigated.
GLP compliance:
no
Type of method:
in vivo

Test material

Constituent 1
Chemical structure
Reference substance name:
Cyclododecanol
EC Number:
217-031-2
EC Name:
Cyclododecanol
Cas Number:
1724-39-6
Molecular formula:
C12H24O
IUPAC Name:
cyclododecanol
Details on test material:
Cyclododecanol from Aldrich Chemical Company, purity not reported

Test animals

Species:
mouse
Strain:
ICR
Sex:
female
Details on test animals or test system and environmental conditions:
TEST ORGANISM
- Female ICR/Ha mice
- Age: 7 weeks - Number: 5-10 animals per group
- Control: diet

Administration / exposure

Route of administration:
oral: feed
Vehicle:
other: semipurified diet
Details on exposure:
TREATMENT - 30 or 50 µmol/g in diet for 2 weeks
Analytical verification of doses or concentrations:
not specified
Duration of treatment / exposure:
2 weeks
Frequency of treatment:
2 weeks
Post exposure period:
no
Doses / concentrations
Remarks:
Doses / Concentrations:
30 or 50 µmol/g
Basis:

No. of animals per sex per dose:
5 - 10
Control animals:
yes, plain diet
Details on study design:
no further details

Examinations

Examinations:
EXAMINATIONS
- Removal and homogenization of liver, forestomach, and mucosa from small  bowel
- GST activity determination using 1-chloro-2,4-dinitrobenzene as  substrate
Positive control:
no positive control

Results and discussion

Details on results:
None of the compounds elicited increased GST activity in the forestomach.  C6 ring compounds showed no significant effect. C12 ring compounds 
were  more effective than C8 ring compounds with a decrease in activity in the  order: unsaturated > epoxide > alcohol > saturated.

Any other information on results incl. tables


-----------------------------------------------------------
Test compound          Liver              Small Bowel Mucosa
                    Specific Activity      Specific Activity
------------------------------------------------------------
None                
 1.96 +- 0.16           0.61 +- 0.04
Cyclododecanol     3.21 +- 0.11*          1.07 +- 0.02*    
                                    * = p <0.005

Applicant's summary and conclusion

Conclusions:
None of the compounds elicited increased GST activity in the forestomach.
Executive summary:

None of the compounds elicited increased GST activity in the forestomach.  C6 ring compounds showed no significant effect. C12 ring compounds  were  more effective than C8 ring compounds with a decrease in activity in the  order: unsaturated > epoxide > alcohol > saturated.