Trisodium [5-acetamido-4-hydroxy-3-[[2-hydroxy-4-[[2-(sulphooxy)ethyl]sulphonyl]phenyl]azo]naphthalene-2,7-disulphonato(5-)]cuprate(3-)

Toxicity to microorganisms

Administrative data

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1990-05-02 to 1990-05-04

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Data source

Reference

Reference 1

Reference Type:

study report

Title:

Unnamed

Year:

1990

Report date:

1990

Reference 2

Reference Type:

other company data

Title:

Unnamed

Year:

1993

Report date:

1993

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

Version / remarks:

version valid 1990

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Copper, 5-(acetylamino)-4-hydroxy-3-[[2-hydroxy-4-[[2-(sulfooxy)ethyl]sulfonyl]phenyl]azo]-2,7-naphthalenedisulfonic acid complex

EC Number:

235-434-1

EC Name:

Copper, 5-(acetylamino)-4-hydroxy-3-[[2-hydroxy-4-[[2-(sulfooxy)ethyl]sulfonyl]phenyl]azo]-2,7-naphthalenedisulfonic acid complex

Cas Number:

12226-38-9

Molecular formula:

C20H17CuN3O15S4

IUPAC Name:

15-acetamido-17,21-disulfo-7-[2-(sulfooxy)ethanesulfonyl]-10λ³,12λ³-dioxa-2λ⁵,3-diaza-11-cuprapentacyclo[11.8.0.0²,¹¹.0⁴,⁹.0¹⁴,¹⁹]henicosa-1(21),2,4,6,8,13,15,17,19-nonaene-11,11-bis(ylium)-10,12-diid-2-yl

Test material form:

not specified

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

activated sludge of a predominantly domestic sewage

Details on inoculum:

- Name and location of sewage treatment plant where inoculum was collected: Kommunale Kläranlage Frankfurt-Sindlingen

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

3 h

Test conditions

Test temperature:

20 +/- 1°C

pH:

7.4 +/- 0.1

Nominal and measured concentrations:

nominal: 1000 and 500 mg/L

Details on test conditions:

TEST SYSTEM
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 3
- Sludge concentration (weight of dry solids per volume):140.0 mg/g
- amount of sludge in test:1.6 g/L

Reference substance (positive control):

yes

Remarks:

3,5-dichlorophenol

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

3 h

Dose descriptor:

EC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Effect concentrations 2

Duration:

3 h

Dose descriptor:

EC10

Effect conc.:

> 500 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Details on results:

- Blank controls oxygen uptake rate: 92%
- Coefficient of variation of oxygen uptake rate in control replicates: 2%

Results with reference substance (positive control):

- Results with reference substance valid? yes
- Relevant effect levels: EC50 between 5 and 30 mg/L

Any other information on results incl. tables

	activity	inhibition
	[mg O2/L_h]	[%]	[mean]
control 1	94.0	-
control 2	90.0	-
control 3	92.0	-
control, mean	92.0	-
reference 5ppm	61.5	33.2
reference 20ppm	22.5	75.5
reference 30ppm	15.0	83.7
1000 mg/L (a)	84.0	8.7
1000 mg/L (b)	78.0	15.2	12.0
500 mg/L (a)	93.0	0.0
500 mg/L (b)	80.0	13.0	6.5

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The 3-h EC50 (respiration inhibition of activated sludge) was determined to be >1000 mg/L. The substance is not inhibitory to activated sludge.