Naphthalene-2-sulphonyl chloride

Skin irritation / corrosion

Administrative data

Endpoint:

skin irritation: in vivo

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

secondary literature

Justification for type of information:

Data is from secondary report.

Data source

Reference

Reference Type:

other: secondary source

Title:

SIDS Initial Assessment Report for 4-Methylbenzenesulfonyl chloride

Author:

United Nations Environmental Programme (UNEP)

Year:

2003

Bibliographic source:

SIDS Initial Assessment Report for 4-Methylbenzenesulfonyl chloride , United Nations Environmental Programme (UNEP) 2003

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 404 (Acute Dermal Irritation / Corrosion)

Principles of method if other than guideline:

To evaluate skin irritation potential of the given test chemical on new Zealand white rabbit.

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

4-Methylbenzenesulfonyl chloride

Cas Number:

98-59-9

Molecular formula:

C7H7ClO2S

IUPAC Name:

4-Methylbenzenesulfonyl chloride

Details on test material:

IUPAC name-4-Methylbenzenesulfonyl chloride
Molecular formula :C7H7ClO2S
Molecular weight :190.65 g/mol
Substance type: Organic
Physical state:solid
Smiles :S(=O)(=O)(c1ccc(cc1)C)Cl
InChI :1S/C7H7ClO2S/c1-6-2-4-7(5-3-6)11(8,9)10/h2-5H,1H3

Specific details on test material used for the study:

IUPAC name-4-Methylbenzenesulfonyl chloride
Molecular formula :C7H7ClO2S
Molecular weight :190.65 g/mol
Substance type: Organic
Physical state:solid
Smiles :S(=O)(=O)(c1ccc(cc1)C)Cl
InChI :1S/C7H7ClO2S/c1-6-2-4-7(5-3-6)11(8,9)10/h2-5H,1H3
Purity: 99.7%
Impurities:≤0.1%

Test animals

Species:

rabbit

Strain:

New Zealand White

Details on test animals or test system and environmental conditions:

- Age at study initiation: 3-4 months old
-Number of animals: 3 animals
-Weight at study initiation: 2.1 - 2.3 kg
-Sex: Male animals

Test system

Type of coverage:

occlusive

Preparation of test site:

abraded

Vehicle:

not specified

Controls:

yes

Amount / concentration applied:

500mg in 0.1 ml sterile distilled water

Duration of treatment / exposure:

4 hrs

Observation period:

1, 24, 48 and 72 hours

Number of animals:

total = 3

Details on study design:

- Area of exposure: (2.5 x 2.5 cm)
-Type of wrap if used: three fold gauze patches
- Washing (if done): washed using warm water
- SCORING SYSTEM: Used (OECD method – Grading of skin reaction)

Results and discussion

In vivo

Results

Results 1

Irritation parameter:

erythema score

Basis:

animal #3

Time point:

72 h

Score:

22

Remarks on result:

positive indication of irritation

Results 2

Irritation parameter:

edema score

Basis:

animal #3

Time point:

72 h

Score:

15

Remarks on result:

positive indication of irritation

Irritant / corrosive response data:

erythema, scab and oedema were observed at the abraded and intact skins on the backs of three rabbits.

Any other information on results incl. tables

Change                                                        Erythema and Eschar
Applicated area	Intact				Abrarded
Phase (hrs)	1	24	48	72	1	24	48	72
Animal ID (M01)	2	3	3	3	2	3	3	3
M02	2	3	3	3	2	3	3	3
M03	2	3	3	3	2	3	3	3
Total	6	9	9	9	6	9	9	9
Mean	2.0	3.0	3.0	2.0	3.0	3.0	3.0	3.0
Total of mean	22.0

Change                                                        Edema
Applicated area	Intact				Abrarded
Phase (hrs)	1	24	48	72	1	24	48	72
Animal ID (M01)	1	2	2	2	2	2	2	2
M02	1	3	2	2	3	2	2	2
M03	1	2	2	1	2	2	2	1
Total	3	7	6	5	6	7	6	5
Mean	1.0	2.3	2.0	1.7	2.0	2.3	2.0	1.7
Total of mean	15.0

Erythema and Eschar formation

0 – No erythema

1 – Very slight erythema (barely perceptible)

2 – Well defined erythema

3 – Moderate to severe erythema

4–Severe erythema (beef redness) to eschar formation preventing grading of erythema

- edema formation

0 – No oedema

1 – Very slight oedema (barely perceptible)

2 – Slight oedema (edges of area well defined by definite raising)

3 – Moderate oedema (raised approximately 1 mm)

4 – Severe oedema (raised more than 1 mm and extending beyond area of exposure)

 

Applicant's summary and conclusion

Interpretation of results:

Category 2 (irritant) based on GHS criteria

Conclusions:

The given test chemical was applied on the rabbit’s back in concentration 500 mg . Erythema, scab and edema were observed at the abraded and intact skins on the backs of three rabbits.Hence, it is considered to be moderately irritating

Executive summary:

The given test chemical was used to evaluate its skin irritation potential on New Zealand White rabbits. 500 mg of test material with about 0.1 mL of sterile distilled water was applied on abraded and intact sites on the shaved backs (2.5 x 2.5 cm) of rabbits. Left side of the back was treated as control. It was held in place for 4 hours with three fold gauze patches, which was applied in place with elasticity bandage then was fixed with non-irritating tape (3M paper-tape). After 4 hours, three fold gauze patches were removed and the exposed areas washed using warm water without altering the existing response or the integrity of the epidermis. The skin of animals was examined in accordance with an OECD scoring method for signs of erythema and oedema and the responses scored at 1, 24, 48 and 72 hours after the patches removal. Test chemical was applied on the rabbit’s back in concentration 500 mg. Erythema, scab and edema were observed at the abraded and intact skins on the backs of three rabbits. Hence, it is considered to be moderately irritating.