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EC number: 249-707-8 | CAS number: 29590-42-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 28 February 1992 to 3 March 1992
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Documentation and inclusion of all necessary parameters per the method utilized and GLP standard. However, recoveries were poor for test solutions, indicating a potential inaccuracy in the actual exposure concentrations.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 992
- Report date:
- 1992
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- yes
- Remarks:
- (1) To control the test substance degradation, the stock solution was prepared daily. (2) The test organisms were fed at 21 h before test initiation.
- GLP compliance:
- yes
Test material
- Reference substance name:
- Isooctyl acrylate
- EC Number:
- 249-707-8
- EC Name:
- Isooctyl acrylate
- Cas Number:
- 29590-42-9
- Molecular formula:
- C11H20O2
- IUPAC Name:
- 2-methylheptyl prop-2-enoate
- Details on test material:
- - Name of test material (as cited in study report): Isooctyl acrylate (CAS No. 29590-42-9)
- Substance type: Pure substance
- Physical state: Liquid
- Analytical purity: 99.75%
- Lot/batch no.: Lot 3290
- Stability under test conditions: Stable in water
- Storage condition of test material: Stored at room temperature in an amber glass bottle and sealed in a metal container
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- Sampling of all test solutions occurred daily, including stock solutions, control solutions, and all exposure concentrations. Samples were collected each day 2 - 4 hours after the stock solution was renewed. Composite samples combining 200 mL of either control or test exposure solution from each replicate respectively were utilized, or individual samples were taken as appropriate. Samples were collected in 250 mL brown glass bottles and combined in 500 mL brown glass bottles. The report states that "the same bottles were used throughout the test.", but it is not clear if that is in reference to reusing bottles, or using new bottles of the same type for each sampling. A clean bottle was used to collect the blank control samples. Samples were combined except at the final time point or where mortality reached 100% in a replicate, whichever came first. It is indicated that analysis occurred daily.
Test solutions
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
Test substance stock solution was prepared daily by adding 260 uL (229 mg) of test substance to 4 L of dilution water. Stock solution was mechanically stirred at ambient temperature for 15 minutes and then sonicated for 15 minutes. This solution was then mixed with 14 L of dilution water in a 19 L glass stock bottle that was preconditioned with a 12 mg/L test substance solution in deionized water. Final volume of stock solution was 18 L. Final concentration was ~12.7 mg/L (nominal). The 19L stock bottle was connected by a glass tube near the bottom of the stock bottle to a pre-calibrated metering pump to deliver the test substance stock solution to the test system.
Volume throughout the test was maintained at 1.5 L. Exposure vessels were kept covered except when sampling or removing organisms.
No evidence of undissolved test substance was noted.
Test organisms
- Test organisms (species):
- Pimephales promelas
- Details on test organisms:
- TEST ORGANISM
- Common name: Fathead minnow
- Source: Aquatic Biosystems Inc., Fort Collins, Colorado
- Age at study initiation (mean and range, SD): Juvenile
- Length at study termination (length definition, mean, range and SD): 1.6 +/- 0.57 cm *
- Weight at study termination (mean and range, SD): 0.025 +/- 0.012 g *
* NOTE: Length and weight of control fish were recorded at study termination.
- Feeding during test: None
ACCLIMATION
- Acclimation period: Fish were held and acclimated for 14 days prior to the test, during which they were exposed to dilution water for the last 9 days.
- Health during acclimation (any mortality observed): Test organisms appeared healthy. 0.6% mortality occurred during holding and acclimation period.
Study design
- Test type:
- flow-through
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
Test conditions
- Hardness:
- 186-187 mg/L (as CaCO3)
- Test temperature:
- 20.4-21.2 C
- pH:
- 8.12-8.25
- Dissolved oxygen:
- 8.4 - 8.7 mg/L
- Nominal and measured concentrations:
- All nominal test concentrations were calculated based on the measured test substance stock solutions, calibration of the metering system, and a dilution factor of 1.8. The mean test substance nominal concentrations were 0.35, 0.62, 1.12, 2.01, and 3.45 mg/L. Mean measured concentrations were 0.09, 0.15, 0.34, 0.82, and 1.75 mg/L respectively.
It was noted that the reported measured concentrations were corrected for daily well water spike recovery (81 +/- 15.4%), and that measured concentrations in exposure samples ranged from 24% to 51% of nominal. It was hypothesized that this discrepancy may be due to an abiotic process such as sorption to glass or volatility. The report states that daily analyses of the samples did not show the presence of test substance degradation products at any noticeable level. No definitive conclusion was offered. - Details on test conditions:
- TEST SYSTEM
- Test vessel: Exposure vessels were 3 Liter rectangular glass tanks measuring 19.5 cm X 9.5 cm x 15.5 cm
- Type: Covered system
- Material, size, headspace, fill volume: 3 L glass tanks containing 1.5 L test solution
- Aeration: Test water was aerated for at least 24 hours prior to use in the test
- Type of flow-through: Proportional diluter
- Renewal rate of test solution (frequency/flow rate): Flow rate was calibrated and adjusted to provide 500 mL of exposure water per hour
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2
- Biomass loading rate: 0.02 g fish/L-day
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Shallow well water collected from the Two Harbors, MN area.
- Total organic carbon: Not given. COD = 9 mg/L
- Particulate matter: TSS < 4 mg/L
- Metals: Zinc 30 ug/L
Sodium 6.5 mg/L
Magnesium 16.3 mg/L
Iron 3 ug/L
Copper 13 ug/L
Calcium 46.5 mg/L
- Pesticides: None detected
- Ca/Mg ratio: 2.85
- Conductivity: 351 to 375 umhos/cm
- Intervals of water quality measurement: Total hardness and alkalinity were measured at test initiation. pH, dissolved oxygen (DO) concentrations, and temperature were monitored daily. Specific conductivity was measured at test initiation and termination.
OTHER TEST CONDITIONS
- Adjustment of pH: None
- Photoperiod: 16 hours light / 8 hours dark using cool white fluorescent lamps
- Light intensity: Not given
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Test organisms were observed for signs of stress and mortality at 3 hours and 6 hours after test initiation, and then observed daily, at which point any dead test organisms were removed from the exposure vessels. - Reference substance (positive control):
- no
Results and discussion
Effect concentrations
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 0.67 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 95% C.I.: 0.56 to 0.82 mg/L
- Details on results:
- - Mortality of control: None
- Other adverse effects control: None
- Abnormal responses: None - Reported statistics and error estimates:
- For LC50 calculations, the test organism mortality data and the test substance mean measured concentrations were analyzed using the trimmed Spearman-Karber method. For the NOEC calculation, the data were analyzed using TOXSTAT statistical software. Each measured test substance concentration was corrected for the well water spike recovery before calculating the mean measured concentration.
Any other information on results incl. tables
- Sublethal observations / clinical signs:
Table 1, Matrix spike recoveries for the test substance Matrix Time of analysis (h) Test substance conc'n (mg/L) Percent recovery nominal measured Matrix ─ Deionized water Method blank 0 0.0 <0.04 ─ 24 0.0 <0.04 ─ 48 0.0 <0.04 ─ 72 0.0 <0.04 ─ 96 0.0 <0.04 ─ Spike solution 0 0.22 0.24 109 24 0.22 0.15 71 48 0.22 0.16 75 72 0.22 0.21 94 96 0.22 0.19 89 Mean spike recovery 88 ± 15.3% Matrix ─ Well water Method blank 0 0.0 <0.04 ─ 24 0.0 <0.04 ─ 48 0.0 <0.04 ─ 72 0.0 <0.04 ─ 96 0.0 <0.04 ─ Spike solution 0 0.22 0.23 104 24 0.22 0.14 63 48 0.22 0.16 74 72 0.22 0.19 86 96 0.22 0.17 77 Mean spike recovery 81 ± 15.4% TEST SOLUTION ANALYSES
Table 2, Nominal and mean measured concentrations of substance during test Test substance nominal conc'n (mg/L) Replicate Test substance measured conc'n (mg/L) 0 hrs 24 hrs 48 hrs Reported Percent of nominal 72 hrs 96 hrs mean ± SD 0.0 (control) A ─ ─ ─ ─ <0.04 B ─ ─ ─ ─ <0.04 Composite <0.04 <0.04 <0.04 <0.04 ─ 0.35 A ─ ─ ─ ─ 0.09 B ─ ─ ─ ─ 0.09 Composite 0.09 0.09 0.10 0.07 0.09±0.008 26 0.62 A ─ ─ ─ ─ 0.14 B ─ ─ ─ ─ 0.14 Composite 0.15 0.17 0.17 0.11 0.15 ± 0.024 24 1.12 A ─ ─ ─ ─ 0.35 B ─ ─ ─ ─ 0.34 Composite 0.34 0.37 0.39 0.23 0.34 ± 0.056 30 2.01 A ─ ─ ─ ─ 0.85 B ─ ─ ─ ─ 0.76 Composite 0.99 0.92 0.89 0.50 0.82 ± 0.174 41 3.45 A ─ 7.64 1.81 ─ ─ B ─ 7.12 1.76 ─ ─ Composite 1.71 1.71 ─ ─ ─ 1.75 ± 0.047 51 FISH DOSE RESPONSE
Table 3, Summary of Acute Toxicity of Isooctyl Acrylate to the Fathead Minnow (Pimephales promelas) Under Flow-Through Conditions Mean Measured Conc., mg/L Percent Mortality 24-hrs 48-hrs 72-hrs 96-hrs Blank Control 0 0 0 0 0.09 0 0 0 0 0.15 0 0 0 0 0.34 0 5 5 5 0.82 0 35 65 65 1.75 5 100 100 100 LC50 Not calculable 0.86 0.67 0.67 95% C.I. Not calculable 0.71 - 1.05 0.56 - 0.82 0.56 - 0.82
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 96-hour LC50 of the test substance to fathead minnow (Pimephales promelas) is 0.67 mg/L. The NOEC, mased on mortality, is 0.34 mg/L.
- Executive summary:
In a 96-h acute toxicity study, fathead minnow (Pimephales promelas) were exposed to isooctyl acrylate (CAS No. 29590-42-9) at nominal concentrations of 0 (control), 0.35, 0.62, 1.12, 2.01, and 3.45 mg/L and mean measured concentrations of 0 (control), 0.09, 0.15, 0.34, 0.82, and 1.75 mg/L under flow-through conditions. The 96-h LC50 was 0.67 mg/L. The NOEC value based on mortality was 0.34 mg/L. Sublethal effects were not observed.
This toxicity study is classified as acceptable and satisfies the guideline requirement for acute fathead minnow toxicity study. The reliability rating is 2 (reliable with restrictions). The study was conducted under GLP with measured concentrations. However, recoveries were poor for test solutions, indicating a potential inaccuracy in the actual exposure concentrations.
Results Synopsis
Test organism size/age: 1.6 cm (+/-0.57) / Juvenile
Test Type: flow-through
LC50: 0.67 mg/L 95% C.I.: 0.56 to 0.82 mg/L
NOEC: 0.34 mg/L
Endpoint(s) Effected: Mortality
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