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EC number: 249-707-8 | CAS number: 29590-42-9
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- June 1980
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Performed by a highly experienced laboratory
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 980
- Report date:
- 1980
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Guideline:
- other: The study was performed in 1980 and the published proceedures by Bruce Ames and others were cited in the references included in the study.
- Deviations:
- not applicable
- Principles of method if other than guideline:
- Standard laboratory practices followed
- GLP compliance:
- no
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- T-2476ChR
- IUPAC Name:
- T-2476ChR
- Reference substance name:
- Isooctyl acrylate
- EC Number:
- 249-707-8
- EC Name:
- Isooctyl acrylate
- Cas Number:
- 29590-42-9
- Molecular formula:
- C11H20O2
- IUPAC Name:
- 2-methylheptyl prop-2-enoate
- Details on test material:
- - Name of test material (as cited in study report: T-2467ChR
- Physical state:Clear Liquid
- Analytical purity: Not reported
- Impurities (identity and concentrations): Not reported
- Stability under test conditions: Stable
- Storage condition of test material: room temperature
Constituent 1
Constituent 2
Method
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Species / strain / cell type:
- other: D3 strain of S. cerevisiae
- Metabolic activation:
- with and without
- Metabolic activation system:
- Aroclor 1254-induced rat liver S-9; SRI Batch #D4, ca. 30mg/mL protein
- Test concentrations with justification for top dose:
- 0.0005 to 5.0 uL/plate; mutiple assays
- Vehicle / solvent:
- Dimethylsulfoxide (DMSO
Controls
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- other: Solvent control only
- Positive controls:
- yes
- Positive control substance:
- sodium azide
- Remarks:
- Migrated to IUCLID6: 2-Anthramine
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in medium; in agar (plate incorporation); preincubation; in suspension; as impregnation on paper disk
DURATION
- Preincubation period: 4 hours
- Exposure duration: 48 hours - Evaluation criteria:
- 3X+ control background
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- other: S. cervisiae D3
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
- TEST-SPECIFIC CONFOUNDING FACTORS
- Other confounding effects: None - Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative expert judgment
T-2476ChR is not mutagenic with S. typhimurium or recombinogenic with S. cerevisiae D3 - Executive summary:
Compound T-2476ChR was tested for mutagenicity with the Ames Salmonella/microsome assay and with the yeast Saccharomyces cerevisiae D3 assay in the presence and in the absence of a metabolic activation system. The compound was tested at least twice on separate days in both assays.
Dimethylsulfoxide (DMSO) was used as the solvent in all assays.
In the Ames Salmonella/microsome assay, Compound T-2476ChR was tested initially in a preliminary assay with S. typhimurium strain TA100 over a wide range of concentrations, from 0.01 to 5.0 ul/plate Pinpoint colonies (indicating toxicity) appeared at 5.0 ul/plate, so the concentration range was lowered to 0.0005 to 0.5 ul/plate in subsequent tests using all five strains of S. typhimurium. At 0.5 ul/plate, the test compound was toxic with TA100, but no dose-related increase in the number of revertants per plate was observed in either assay.
In the microbiological assays with S. cerevisiae D3, T-2476ChR was initially tested over a wide range of concentrations, from 0.01 to 5.0% Toxicity was observed at all concentrations so the range was lowered to 0.00005 to 0.05% for subsequent testing. No dose-related increase in the number of mitotic recombinants above background was observed.
The test material, T-2476ChR was negative in this assay.
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