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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
from 1990-05-04 to 1990-07-10
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Version / remarks:
1984
Deviations:
no
GLP compliance:
yes
Analytical monitoring:
no
Details on sampling:
- No concentrations of the test substance were measured.
Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: The test was prepared by mechanical dispersion dissolving the appropriate amount of the test substance in the required volume of deionised water.
- Controls: Blank control performed with the test solution without the test substance.
- Evidence of undissolved material: No
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Water flea
- Age at study initiation: not older than 24 hours
- Source: Institute of Toxicology, Merck KGaA, Darmstadt, Germany
- Feeding during test: no
- Aeration during the test: no

ACCLIMATION
- Acclimation conditions:same as the tet conditions
- Feeding during the acclimation: fresh algae (Chlorella vulgaris)
- Health during acclimation: No mortality was observed during the test
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Hardness:
341 mg CaCO3/L
Test temperature:
20.5 °C
pH:
7.9
Dissolved oxygen:
94 - 100 %
Nominal and measured concentrations:
- Nominal concentrations: 0 (control) 1, 10 and 100 mg/L.
- Measured concentrations: No concentrations were measured
Details on test conditions:
TEST SYSTEM
- Test vessel: all-glass
- Aeration: no
- No. of organisms: 20

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Reconstituted water according to OECD 202 (1984)
- Culture medium different from test medium: No

EFFECT PARAMETERS MEASURED: Immobility of the animals after 24, 48 hours

VEHICLE CONTROL PERFORMED: no

RANGE-FINDING STUDY
not performed
Reference substance (positive control):
no
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 10 mg/L
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
>= 10 mg/L
Details on results:
- Behavioural abnormalities: not observed
- Observations on body length and weight: not observed
- Other biological observations: not observed
- Mortality of control: no
- Other adverse effects control: not observed
- Immobilisation of control: not observed
- Abnormal responses: not observed
- Any observations that might cause a difference between measured and nominal values: no
- Effect concentrations exceeding solubility of substance in test medium: no
Results with reference substance (positive control):
not performed
Validity criteria fulfilled:
yes
Conclusions:
The EC50 (48h) of the test item to Daphnia magna was determined to be > 10 mg/L (reference 6.1.3-1) according to OECD 202 under GLP conditions.
Executive summary:

The objective of this study was to determine the acute toxicity of the test material to Daphnia magna according to OECD 202 (1984) under GLP conditions. For this purpose, young Daphnia magna were exposed to aqueous test material concentrations under defined conditions. Daphnia were exposed to aqueous test material solutions of nominal 0 (control), 1 and 10.0 mg/L in a static system. Due to the low solubility of the test substance (9 mg/L) no analytical measured were performed. The guideline does not demand the investigation of higher concentrations than the water solubility. However, 100 mg/L were tested, too. In this concentration 40 % of the Daphnia were immobilized after 24 h. This may be due to physical effects (e.g. sticking of the antennae) rather than to toxic effects of the test substance were determined. Daphnia were observed for immobilization up to 48 hours. A blank control without test substance was performed. No test with reference substance as positive control was determined.  EC50 (48h) of the test item to Daphnia magna was determined to be > 10 mg/L.

Description of key information

The EC50 (48h) of the test item to Daphnia magna was determined to be > 10 mg/L (reference 6.1.3-1) according to OECD 202 under GLP conditions.

Key value for chemical safety assessment

Additional information

The objective of this study was to determine the acute toxicity of the test material to Daphnia magna according to OECD 202 (1984) under GLP conditions. For this purpose, young Daphnia magna were exposed to aqueous test material concentrations under defined conditions. Daphnia were exposed to aqueous test material solutions of nominal 0 (control), 1 and 10.0 mg/L in a static system. Due to the low solubility of the test substance (9 mg/L) no analytical measured were performed. The guideline does not demand the investigation of higher concentrations than the water solubility. However, 100 mg/L were tested, too. In this concentration 40 % of the Daphnia were immobilized after 24 h. This may be due to physical effects (e.g. sticking of the antennae) rather than to toxic effects of the test substance were determined. Daphnia were observed for immobilization up to 48 hours. A blank control without test substance was performed. No test with reference substance as positive control was determined.  EC50 (48h) of the test item to Daphnia magna was determined to be > 10 mg/L.

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