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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

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Administrative data

Link to relevant study record(s)

Reference
Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2008-02 to 2008-12
Reliability:
1 (reliable without restriction)
Qualifier:
according to guideline
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
Deviations:
no
GLP compliance:
yes (incl. QA statement)
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
PHYSICO-CHEMICAL PROPERTIES
- Melting point: 69°C
- Flash point: 243.5°C
- Vapour pressure: no data
- Water solubility (under test conditions): <1mg/L
- Henry's law constant: no data
- log Pow: 13.2
Analytical monitoring:
yes
Vehicle:
no
Test organisms (species):
Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
Details on test organisms:
TEST ORGANISM
- Common name: Pseudokirchnerella sucapitata
- Strain: Chlorococcal order, CCAP 278,4
- Source (laboratory, culture collection): FCBA laboratory

ACCLIMATION
- Acclimation period:4 days
- Culturing media and conditions (same as test or not):same as test
- Any deformed or abnormal cells observed:before inoculation, the algae are controled under microscop with heamo.cytometer
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
72 h
Post exposure observation period:
control of the algae growth inhibition every 24 hours
Test temperature:
23 to 23.4°C
pH:
7.9
Salinity:
CaCL2, 2H2O=18mg/L
MgCL2, 6H20=12mg/L
NH4Cl=15mg/L
KH2PO4=1.6mg/L
Na2EDTA, 2H2O=0.1mg/L
MnCl2, 4H2O=0,415mg/L
COCl2, 6H2O=1,5µg/L
NaMoO4, 2H20=7µg/L
MgSO4, 7H2O=15mg/L
FeCl3, 6H2O=0.08mg/L
H3BO3=0.185mg/L
ZnCl2=3µg/L
CuCl2, 2H2O=0,01µg/L
NaHCO3=15mg/L


Nominal and measured concentrations:
1, 10; 100mg/L
Details on test conditions:
TEST SYSTEM

- No. of organisms per vessel:1000algae /ml
- No. of vessels per concentration (replicates):3
- No. of vessels per control (replicates):6
- No. of vessels per vehicle control (replicates):6

GROWTH MEDIUM
- Standard medium used: yes

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: electronic particle counter

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 10
Reference substance (positive control):
yes
Remarks:
potassium dichromate
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Results with reference substance (positive control):
- Results with reference substance valid?
- EC50: after 72hours is 0.69mg/L
Validity criteria fulfilled:
yes
Conclusions:
EC50-72Hours >100mg/L (value expressed in test item nominal concentration)
Executive summary:

The toxicity of the test item "LCA07033" was performzed on the freshwater unicellular green algae Pseudokirchnerella subcapitata

according to the OECD 201 guideline (screening test).

Results obtained show aslight toxicity of the test item with an inhibition of the average specific growth rate in comparison to the control <50% at the highest concentration of 100mg/L. For that reason, ErC50(0 -72h) is superior to this highest tested concentration.

Analyses of the test solutions were performed:the Dipalmitoyl Hydroxyproline was analysed inAlgae WAF at the start and the end of the study: Results are presented as follow/

 Nominal test item concentrations in mg/L   
   [DPHP]
           at 0h    at 72h
 unfiltered control  <LOD  <LOD
 100  58,8µg/L  74,9µg/L

 

Description of key information

Key value for chemical safety assessment

EC50 for freshwater algae:
100 mg/L

Additional information