2,4-Imidazolidinedione, 5-(2-methylpropylidene)-, (5Z)-

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2020

Report date:

2020

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

other: The editorial board of “The Guidelines for the Testing of Chemicals ”of Chemical Registration Center of the Ministry of Environmental Protection.

Version / remarks:

The Guidelines for the Testing of Chemicals, Effects on Biotic Systems, 203 Fish Acute Toxicity Test [M]. Second Edition Beijing: China Environmental Press. 2013, page 30-36

Test guideline 2

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Test guideline 3

Qualifier:

according to guideline

Guideline:

other: National Standardization Technical Committee for the Management of Hazardous Chemicals. GB/T 27861-2011 Chemicals Fish Acute Toxicity Test [S]. Beijing: China Standard Press. 2012.

Test guideline 4

Qualifier:

according to guideline

Guideline:

other: National Standardization Technical Committee for the Management of Hazardous Chemicals. GB/T 29763-2013 Chemicals Rare Minnow (Gobiocypris rarus) Acute Toxicity Test [S]. Beijing: China Standard Press. 2013.

GLP compliance:

yes

Remarks:

the study was conducted in compliance with the Guideline of Chemical Testing Good Laboratory Practices (HJ/T 155-2004) and Principles of Good Laboratory Practices (GB/T 22278-2008)

Test material

Test material information

Constituent 1

Reference substance name:

(5Z)-5-(2-methylpropylidene)imidazolidine-2,4-dione

EC Number:

700-726-4

Cas Number:

1369499-44-4

Molecular formula:

C7H10N2O2

IUPAC Name:

(5Z)-5-(2-methylpropylidene)imidazolidine-2,4-dione

Test material form:

solid

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

Samples were taken for analysis at the beginning of exposure (0 h) and at the end of exposure (96 h). On each occasion, three samples were taken from test item treatment group (one sample was retained in case further analysis was required) and one from blank control.

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

other: Gobiocypris rarus (rare minnow)

Details on test organisms:

TEST ORGANISM
- Common name: rare minnow (Gobiocypris rarus)
- Source: The rare minnow was initially derived from Institute of Hydrobiology, Chinese Academy of Science, and then had been reproduced and maintaining in the facility of CTI-SET. The code number of fish used in this test was rare minnow C20200430
- Length at study initiation (length definition, mean, range and SD): 2.0-4.0 cm
- Weight at study initiation (mean and range, SD): wet weight of fish was 300 ± 12 mg (mean ± SD)

ACCLIMATION
- Acclimation period: 7-day acclimation before the test
- Acclimation conditions (same as test or not): same as test
- Feeding frequency during acclimation: daily until 24 hours before the test
- Health during acclimation (any mortality observed): no mortality

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Test conditions

Hardness:

140 mg CaCO3/L

Test temperature:

temperature was maintained in the range of 23.0-23.5 °C

pH:

7.72-8.19

Dissolved oxygen:

dissolved oxygen was in the range of 67.2 %-98.9 % ASV

Nominal and measured concentrations:

nominal 100 mg/L
arithmetic mean measured 94 mg/L

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 3

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 94 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Any other information on results incl. tables

Sublethal observations / clinical signs:

Accumulative Mortality in Definitive Test

Nominal concentration (mg/L)	Accumulative mortality (initial fish number=7)					96h mortality (%)
	3 h	24 h	48 h	72 h	96 h
Blank control	0	0	0	0	0	0
100	0	0	0	0	0	0

Sub-lethal Effects of Exposure in Definitive Test 

Nominal concentration (mg/L)	Number of fish with sub-lethal effects (initial fish number=7)
	3 h	24 h	48 h	72 h	96 h
Blank control	0	0	0	0	0
100	0	0	0	0	0

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Based on the nominal concentration, the 96h-LC50 of test item to rare minnow was >100 mg/L (the arithmetic mean of measured concentration was > 94 mg/L).