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EC number: 601-478-9
CAS number: 117428-22-5
Eisenia fetida (clitellate adults) were exposed to concentrations of the
test substance in an artificial soil for 14 days at 20 ± 2°C. An initial
and a definitive test were conducted. The test was conducted according
to OECD guideline 207.
The value for the 14 day LC50 was 6.7 mg a.i./kg soil. At the 3.2 mg
a.i./kg soil level, there were no deaths, behavioural effects,
significant effects on external conditions or significant body weight
changes (p = 5%) and thus, the No Observed Effect Level (NOEL) was at
least 3.2 mg a.i./kg soil.
The LC50 value for 2-chloroacetamide, tested as a toxic standard, was 52
The sublethal toxicity of test substance to earthworms, Eisenia fetida
Savigny, was estimated in a 56-day soil exposure GLP-compliant
laboratory study based on OECD 222 (2004) and ISO 11268, Part 2 (2012).
Eight replicates for the control and four replicates for the test item
groups of ten clitellated adult earthworms each were exposed to
artificial soil (prepared according to OECD 222) treated with the test
item to obtain the nominal concentrations of 0.293, 0.439, 0.658, 0.988,
1.48, 2.22, 3.33 and 5.00 mg picoxystrobin/kg dry artificial soil and to
an untreated control (using the same amount of acetone and quartz sand
as in the test item treated groups moistened with deionized water).
The reference item (active substance carbendazim) is tested at least
once a year at five concentrations.
Adult earthworms were sorted from soil and assessed for mortality,
weight loss, and sublethal effects after 28 days. Soil was replaced in
the test container and juveniles were allowed to grow for another 28
days, at which time they were removed from the soil and counted. Food
consumption per test container was observed and tallied throughout the
All study validity criteria were met.
The mortality of 72.5% at the concentration of 5.00 mg test substance/kg
dry artificial soil was statistically significantly different compared
to the control (Fisher’s Exact Test, α = 0.05). No additional mortality
was observed. The LC50 after 28 days was determined to be 4.80 mg test
substance/kg dry artificial soil.
The body weight changes were not statistically significantly different
compared to the control up to and including the concentration of 2.22 mg
test substance/kg dry artificial soil. At 3.33 mg test substance/kg dry
artificial soil and above the weight increase was statistically
significantly lower compared to the control (Williams t-test, α = 0.05,
two-sided). For the evaluation of the weight changes the highest
concentration was not included due to the low number of surviving worms.
No statistically significant effects on reproduction were observed up to
and including the concentration of 1.48 mg test substance/kg dry
artificial soil. At 2.22 mg test substance/kg dry artificial soil and
above reproduction was statistically significantly reduced compared to
the control (Williams t-test, α = 0.05, one-sided smaller).
No behavioral abnormalities were observed in any treatment group in this
The NOEC (No-Observed-Effect Concentration) for mortality was determined
to be 3.33 mg test substance/kg dry artificial soil. The NOEC for weight
changes was determined to be 2.22 mg tes substance/kg dry artificial
soil. The NOEC for reproduction was determined to be 1.48 mg test
substance/kg dry artificial soil; therefore the overall NOEC of test
substance to the earthworm Eisenia fetida was determined to be 1.48 mg
test substance/kg dry artificial soil.
The LC50 after 28 days was determined to be 4.80 mg test substance/kg
dry artificial soil. The EC10 for reproduction was determined to be 1.97
mg test substance/kg dry artificial soil; the EC20 was 2.30 mg
substance/kg dry artificial soil and the EC50 was 3.09 mg substance/kg
dry artificial soil.
56-day NOEC (Eisenia fetida): 1.48 mg/kg soil dw (reproduction); OECD
222; Reliability = 1
14-day LC50 (Eisenia fetida): 6.7 mg a.i./kg; OECD 207; Reliability = 1
14-day NOEL (Eisenia fetida): 3.2 mg a.i./kg; OECD 207; Reliability = 1
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