Diammonium 5-(hexylsulfonyl)-2-{[3-methyl-2,7-dioxo-1-(3-sulfonatobenzoyl)-2,7-dihydro-3H-naphtho[1,2,3-de]heterocycle-6-yl]amino}benzenesulfonate

Adsorption / desorption

Administrative data

Endpoint:

adsorption / desorption: screening

Type of information:

experimental study

Adequacy of study:

key study

Study period:

08 January 2009 to 26 February 2009

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Study conducted in compliance with agreed protocols, with no or minor deviations from standard test guidelines and/or minor methodological deficiencies, which do not affect the quality of relevant results.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2009

Report date:

2009

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

EU Method C.19 (Estimation of the Adsorption Coefficient (KOC) on Soil and Sewage Sludge Using High Performance Liquid Chromatography (HPLC))

Deviations:

yes

Remarks:

Testing was not possible on its (essentially) unionised state.

Principles of method if other than guideline:

As the test material was noted to contain numerous salted sulphonic acid groups, testing was carried out at pH 7, with the test material in its fully ionised state.

Also, as a test pH of approximately -1.5 would be required to test the substance in its most unionised state [due to its acidic and basic dissociation constants, it was considered that testing was not possible on its (essentially) unionised state.

GLP compliance:

yes (incl. QA statement)

Remarks:

Date of inspection: 19 August 2008; Date of signature: 04 March 2009

Type of method:

HPLC estimation method

Media:

soil/sewage sludge

Test material

Test material information

Radiolabelling:

not specified

Results and discussion

Adsorption coefficient

Key result

Type:

log Koc

Value:

> 0 - < 1.25 dimensionless

pH:

7

Temp.:

30 °C

Any other information on results incl. tables

Calibration

The retention times of formamide and the retention times, capacity factors (k) and log₁₀ K_oc values for the reference standards are shown in the two following tables:

Table 1

Dead Time	Retention Time (mins)		Mean Retention Time (mins)
	Injection 1	Injection 2
Formamide	3.348	3.350	3.349

Table 2

Standard	Retention Time (mins)		Mean Retention Time (mins)	Capacity Factor (k)	Log10 k	Log10Koc
	Injection 1	Injection 2
Acetanilide	4.068	4.067	4.068	0.215	-0.668	1.25
Phenol	4.066	4.067	4.067	0.214	-0.669	1.32
Atrazine	4.480	4.480	4.480	0.338	-0.471	1.81
Isoproturon	4.854	4.854	4.854	0.449	-0.347	1.86
Triadimenol	5.195	5.197	5.196	0.552	-0.258	2.40
Linuron	6.070	6.061	6.066	0.811	-9.09 x 10^-2	2.59
Naphthalene	7.754	7.755	7.755	1.32	0.119	2.75
Endosulfan-diol	5.993	6.003	5.998	0.791	-0.102	3.02
Fenthion	10.098	10.097	10.098	2.02	0.304	3.31
a-Endosulfan	10.291	10.259	10.260	2.06	0.315	4.09
Phenanthrene	13.676	13.668	13.672	3.08	0.489	4.09
Diclofop-methyl	12.321	12.313	12.317	2.68	0.428	4.20
DDT	29.518	29.503	29.511	7.81	0.893	5.63

Adsorption coefficient of sample

The retention times, capacity factors and log₁₀ K_oc values determined for the sample are shown in the following table:

Table 3

Injection	Retention Time (mins)	Capacity Factor (k)	Log10 k	Log10 Koc
1	1.726	<0.215	<-0.668	<1.25
2	1.723	<0.215	<-0.668	<1.25

Mean log₁₀ K_oc:                   <1.25
Adsorption coefficient:        <17.8

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The adsorption coefficient (Koc) of the test material has been determined to be less than 17.8, log10 Koc <1.25.

Executive summary:

Less than 17.8, log₁₀ K_oc <1.25, using the HPLC screening method, Method C19 Adsorption Coefficient of Commission Regulation (EC) No 440/2008 of 30 May 2008.