Isodecyl diphenyl phosphate

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

GLP compliance:

yes

Specific details on test material used for the study:

Purity: >90%
Batch: 900150909STG115

Analytical monitoring:

yes

Details on sampling:

Analysis of the fresh test preparations was carried out at 0 and 72 hours. Analysis of the old test preparations was carried out at 24 and 96 hours.

Vehicle:

yes

Remarks:

dimethylformamide

Details on test solutions:

A solvent stock solution was prepared by dissolving a nominal amount of test item (100 mg) in a final volume of 10 mL of dimethylformamide. An aliquot (2.0 mL) of the stock solution was dispersed in 20 liters of test water with the aid of propeller stirring for 30 minutes to give the
1.0 mg/L test concentration.

The test item preparations were observed to be a slightly hazy dispersions throughout the test.

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

Fish were obtained from Brow Well Fisheries Limited, Hebden, near Skipton, Yorkshire, UK and maintained in house since 18 February 2016.

Test type:

semi-static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Hardness:

Laboratory tap water was dechlorinated by passage through an activated carbon filter (Purite Series 500) and partly softened (Elga Nimbus 1248D Duplex Water Softener) giving water with a total hardness of approximately 140 mg/L as CaCO3

Test temperature:

13-14 deg C

pH:

7.4-8.0

Dissolved oxygen:

11.6-9.1 mgO2/L

Reference substance (positive control):

no

Key result

Duration:

96 h

Dose descriptor:

EC50

Effect conc.:

> 0.15 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat. (total fraction)

Basis for effect:

mortality (fish)

Sublethal observations / clinical signs:

Geometric Mean Measured Concentration (mg/L)	Cumulative Mortality (Initial Population = 7)							% Mortality
	1 Hours	3 Hours	6 Hours	24 Hours	48 Hours	72 Hours	96 Hours	96 Hours
Control	0	0	0	0	0	0	0	0
Solvent Control	0	0	0	0	0	0	0	0
0.15	0	0	0	0	0	0	0	0

Validity criteria fulfilled:

yes

Conclusions:

The exposure of O. mykiss to the test item at a concentration of 0.15mg/L showed no effects. As the tested concentraion is above the water solubility of the substance and no effects were seen, the substance is not considered acutely toxic.

Executive summary:

The substance was tested following the methods outline in the OECD 203 guideline. The test was conducted to GLP standards and fulfilled the validity criteria. The exposure of O. mykiss to the test item at a concentration of 0.15mg/L showed no effects. As the tested concentraion is above the water solubility of the substance and no effects were seen, the substance is not considered acutely toxic.

Description of key information

The substance was tested following the methods outline in the OECD 203 guideline. The test was conducted to GLP standards and fulfilled the validity criteria. The exposure of O. mykiss to the test item at a concentration of 0.15mg/L showed no effects. As the tested concentraion is above the water solubility of the substance and no effects were seen, the substance is not considered acutely toxic.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

0.15 mg/L

Additional information

The available studies on the short-term fish toxicity of IDDPP are listed in the table below:

 

Guideline	Test species	Endpoint	Result	Remarks
EPA-660/3-75-009	Salmo gairdneri	96h-LC50	7.6 mg/l (nominal) S-148	Study study, GLP, rel. 2
EPA-660/3-75-009	Pimephales promelas	96h-LC50	18 mg/l (nominal) S-148	Supporting study, GLP, rel. 2
No data	Lepomis macrochirus	96h-TL50	> 100 mg/l (nominal) S-148	Supporting study, rel. 3
No data	Oncorhynchus mykiss	96h-TL50	>10 - <100 mg/l S-148	Supporting study, rel. 3
American Society for Testing and Materials. 1980. The annual book of ASTM standards: standard practice for conducting acute toxicity tests with fishes, macroinvertebrates and amphibians. Designation E719-80. Philadelphia, PA.	Oncorhynchus mykiss	96h-LC50	26 mg/l (nominal) Santicizer 148	Supporting study, rel. 2

American Society for Testing and Materials. 1980. The annual book of ASTM standards: standard practice for conducting acute toxicity tests with fishes, macroinvertebrates and amphibians. Designation E719-80. Philadelphia, PA.	Lepomis macrochirus	96h-LC50	72 mg/l (nominal) Santicizer 148	Supporting study, rel. 2
OECD 203 Short-term toxicity to fish, Envigo 2016	Oncorhynchus mykiss	96 hr LC50	>0.15 mg/L (geomean)	Key Study, GLP, Rel. 1

As all measured toxicity values are well above the water solubility (11.2 ug/l) of the substance, IDDPP can not be classified as toxic to the environment.