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Physical & Chemical properties

Water solubility

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Reference
Endpoint:
water solubility
Type of information:
experimental study
Adequacy of study:
key study
Study period:
From May 23rd to May 25th, 2016.
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 105 (Water Solubility)
Deviations:
no
Qualifier:
according to guideline
Guideline:
EU Method A.6 (Water Solubility)
Deviations:
no
GLP compliance:
yes (incl. QA statement)
Type of method:
column elution method
Key result
Water solubility:
0.19 mg/L
Conc. based on:
test mat.
Incubation duration:
72 h
Temp.:
20 °C
pH:
>= 5.98 - <= 6.02
Details on results:
Solubility of the test item in distilled water is 0.019 ± 0.005 mg/L at pH = 6.04 - 6.08.

Table 1. Results of the preliminary test

0.01 g in x mL of water

0.05

0.1

0.2

1

10

100

200

1000

>1000

Soluble (+/-)

-

-

-

-

-

-

-

-

+

Approximate solubility,
g/L

> 200

200
to
100

100
to
50

50
to
10

10
to
1

1
to
0.1

0.1
to
0.05

0.05
to
0.01

< 0.01

The final approximate solubility was found to be < 0.01 g/L.

 

Table 2. Study solution – first run.

Sample

Sample volume

Absorbance Ap

X [mg/L]

1

15

0.259

0.228

2

15

0.26

0.229

3

15

0.254

0.224

4

15

0.253

0.223

5

15

0.247

0.218
 6  15  0.246  0.217


Table 3. Study solution – second run.

Sample

Sample volume

Absorbance Ap

[mg/L] X

1

15

0.191

0.168

2

15

0.191

0.168

3

15

0.187

0.164

4

15

0.187

0.164

5

15

0.183

0.161
 6  15  0.188  0.165


Table 4. Results of Diosmetin solubility at 20 ± 0.5 ºC in distilled water, column elution with recirculating pump method.

 

1

2

3

4

5

6

Average
concentration
of Diosmetin
on successive
runs, mg/L

Average
concentration
of Diosmetin,
mg/L

1
run

Concentration
of Diosmetin,
mg/L

0.23

0.23

0.22

0.22

0.22

0.22

0.22 ± 0.01

0.19 ± 0.04

pH

6.01

6.00

6.00

6.02

6.01

6.00

2
run

Concentration
of Diosmetin,
mg/L

0.17

0.17

0.16

0.16

0.16

0.17

0.17 ± 0.003

pH

5.99

5.98

6.00

5.98

6.01

5.99
Conclusions:
The solubility of the test item at 20 ± 0.5 ºC and at pH ranging 5.98 - 6.02 in water is 0.19 ± 0.004 mg/L.
Executive summary:

The study of the water solubility of the test item was performed by the column elution method, according to OECD 105 / EU Method A.6 (GLP study). The test item was dissolved in acetone and added to innert support material. Then, the solvent was evaporated, and the loaded support material was soaked for 2h in 5 ml of water. This suspension was added to a micro-column and the test item was eluted until the concentration reached a plateau. The mass concentration of the eluate at this time was determined by UVVIS spectrophotometry, and taken as the water solubility value. Based on the test results, the solubility of the test item in water of the test item at 20 ± 0.5 ºC and at pH ranging 5.98 - 6.02 in water was found to be 0.19 ± 0.004 mg/L.

Description of key information

Key study. Method according to OECD 105 / EU Method A.6 (column elution method), GLP study. The solubility of the test item in water of the test item at 20 ± 0.5 ºC and at pH ranging 5.98 - 6.02 in water was found to be 0.19 ± 0.004 mg/L.

Key value for chemical safety assessment

Water solubility:
0.019 mg/L
at the temperature of:
20 °C

Additional information

The study of the water solubility of the test item was performed by the column elution method, according to OECD 105 / EU Method A.6 (GLP study). The test item was dissolved in acetone and added to innert support material. Then, the solvent was evaporated, and the loaded support material was soaked for 2h in 5 ml of water. This suspension was added to a micro-column and the test item was eluted until the concentration reached a plateau. The mass concentration of the eluate at this time was determined by UV-VIS spectrophotometry, and taken as the water solubility value. Based on the test results, the solubility of the test item in water of the test item at 20 ± 0.5 ºC and at pH ranging 5.98 - 6.02 in water was found to be 0.19 ± 0.004 mg/L.