1,2,3,4-tetrahydronaphthalene

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1995-04-10 to 1995-04-14

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Guideline study with acceptable restrictions: Dissolved oxygen found low in several solutions; solvent concentration exceeds recommended concentration

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Version / remarks:

Cited as Directive 92/69/EEC, C.1

GLP compliance:

yes

Analytical monitoring:

yes

Details on sampling:

The test item concentration was measured before the start of the study (0 h)
The analysis of one additional replicate  per concentration level was repeated after 24 hours (parallel sample  without fish)

Vehicle:

yes

Details on test solutions:

- Vehicle, solvent: 10 g test substance/L ethanol (abs.)
- Maximum concentration of vehicle/ solvent: 1.4 ml ethanol/L

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

- Supplier: West Aquarium, Bad Lauterberg (Germany)
- Wild caught: no - Age/size/weight/loading: 3 cm +/- 0.5 cm
- Feeding: daily 1 % of body weight TetraMin
- Pretreatment: Three times per week treatment with malachite green  followed by 14 days under quarantine
- Feeding during test: no

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Hardness:

ca. 12 degree dH

Test temperature:

20 degree °C (constant)

pH:

7.7 - 8.0 (no adjustment of pH)

Dissolved oxygen:

13 - 102 % saturation.

Nominal and measured concentrations:

3.5, 5.0, 7.0, 10, 14 mg/l (nominal)   
1.5, 2.4, 3.7, 9.1, 7.9 mg/l (geometric mean of two analyses):  
 (1) 1.9, 2.8, 5.4, 9.8, 9.9 mg/l (beginning of test)   
(2) 1.2, 2.1, 2.6, 8.4, 6.3 mg/l (stability control)   
The geometric mean analytical concentrations were used for the  evaluation.

Details on test conditions:

- Dilution water: tap water (Gelsenwasser AG)
- Aeration: no (test vessels covered)
- Renewal of test solution: daily, freshly prepared
- Exposure vessel type: ca. 20 l aquarium with 10 l test solution
- Number of replicates, fish per replicate: 1, 10
- Photoperiod: 16 hours light / 8 hours dark 

Reference substance (positive control):

not specified

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

2.4 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

3.2 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: CL 2.4-3.7

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

7.9 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

24 h

Dose descriptor:

LC50

Effect conc.:

> 9.1 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: not available

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

5.5 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 3.7-7.9

Duration:

72 h

Dose descriptor:

LC50

Effect conc.:

3.5 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 2.4-3.7

Details on results:

Control: 0 % dead
Solvent: 0 % dead
A: 1.5 mg/l: 0 % dead  
B: 2.4 mg/l: 0 % dead  
C: 3.7 mg/l: 80 % dead (6 died on day 3, 2 on day 4)
D: 9.1 mg/l: 100 % dead (all fish died on day 2)
E: 7.9 mg/l: 100 % dead (all fish died on day 2)  

The results are based on the geometrical means (0 and 24 h) of the meassured concentrations

Validity criteria fulfilled:

yes

Conclusions:

The LC50 was determined as 3.2 mg/L indicating that the test item may be toxic to fish.

Executive summary:

Brachydanio rerio, were exposed to tetrahydronaphthalene at nominal concentrations ranging from 3.5 to 14 mg/l under static conditions for a period of 96 hours according to EU method C.1. The following endpoint was derived:

LC₅₀: 3.2 mg/L
Endpoint(s) monitored: mortality

The study was classified as "reliable with restrictions".

Description of key information

The aquatic effects of Tetrahydronaphthalene on fish were tested in a 96h semi-static study with Brachydanio rerio according to Directive 92/96/ECC, C.1. (Scholz, 1995). A series of 5 concentrations were tested: 3.5, 5.0, 7.0, 10 and 14 mg/L (geometric mean of two analysis after 0 and 24 hours: 1.5, 2.4, 3.7, 9.1 and 7.9 mg/L ). The results are based on the geometrical means of the test concentrations measured at 0 and 24 h since the test item concentration deviated by more than 20% after 24 hours. The LC50(96h) was determined to be 3.2 mg/L.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

3.2 mg/L

Additional information

The lowest LC50 value of 3.2 mg/L was estimated after 96h by graphical / visual evaluation. The LC0 (equivalent to NOEC) was determined as 2.4 mg/l and the LC100 as 7.9 mg/l.