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EC number: 204-340-2 | CAS number: 119-64-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Version / remarks:
- Cited as Directive 92/69/EEC, C.3
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- Parallel samples without algae were analysed for the test item after 0 and 72 hours
- Vehicle:
- no
- Details on test solutions:
- 1 g test substance/l was stirred in deionized water for 18 hours and filtered. The resulting concentration was 63 mg/l. The maximum concentration tested was 17 mg/l. No vehicle or solvent was used.
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- - Strain: Scenedesmus subspicatus (CHODAT (86.81 SAG))
- Source/supplier: Origin: Institute "Wasser-, Boden- und Lufthygiene", Berlin; further bred inhouse
- Laboratory culture: A preculture is seeded from a stock culture by transfer 3 days prior to begin of test. Test cultures were established from these
precultures.
- Controls: yes (0 mg test substance/l)
- Initial cell concentration: approximately 20,000 cells/ml - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Test temperature:
- 24 +/- 2 degree C
- pH:
- 8.8-8.9 (study start);
7.9-9.4 (end of study) - Nominal and measured concentrations:
- 1.20; 2.0; 3.6; 6.0; 10.0; 17.0 mg/l (nominal)
1.05; 1.8; 3.3; 5.7; 9.1; 16.1 mg/l (measured at 0 hours)
0.99; 1.7; 3.2; 5.4; 8.7; 15.1 mg/l (measured at 72 hours)
Since the analytical concentrations after 0 hours deviated by less than 20% from the nominal concentrations and the values at 72 hours indicate stability, nominal concentrations were used for the evaluation. - Details on test conditions:
- - Renewal of test solution: no
- Exposure vessel type: Sterile-aerated Erlenmeyer flasks on light benches
- Number of replicates: 5 (test substance) or 8 (control)
- Intensity of irradiation: approx. 8000 lux, white
TEST PARAMETER: Light absorption at 685 nm, related to cell density via calibration curve - Reference substance (positive control):
- no
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 3.6 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- cell number
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 3.8 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- cell number
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 7 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- cell number
- Duration:
- 72 h
- Dose descriptor:
- EC90
- Effect conc.:
- 13 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- cell number
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 5.3 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 11 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC90
- Effect conc.:
- 25 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: extrapolated
- Details on results:
- Statistical method: Probit analysis (Cavalli-Sforza, 1972)
- Validity criteria fulfilled:
- not specified
- Conclusions:
- The lowest 72 h-EC50 (biomass) was determined as 7.0 mg/L, 72 h-EC50 (growthrate) was determined as 11.0 mg/L indicating that the test item may be toxic to algae.
- Executive summary:
In a 72 hour toxicity study, the cultures of Scenedesmus subspicatus were exposed to Tetrahydronaphthalene at nominal concentrations of 1.20; 2.0; 3.6; 6.0; 10.0 and 17.0 mg/L under static conditions in accordance with EU Method C3. The following endpoints were derived:
Growth rate:
72 hr ErC50: 11.0 mg/L 95% CL: n.a.72 hr ErC10: 5.3 mg/L 95% CL: n.a.
72 hr NOEC: not available
Biomass:72 hr EbC50: 7.0 mg/L 95% CL.: n.a.
72 hr EbC10: 3.8 mg/L 95% CL.: n.a.
72h NOEC. 3.6 mg/L
The study is classified as "reliable without restrictions".
Reference
RESULTS: EXPOSED AND CONTROL
- Cell density data:
Cells/ml after 0; 24; 48; 72 hours (mean)
-----------------------------------------
Control: 2; 6; 22; 90 (x 10,000)
1.2 mg/l: 2; 7; 27; 108 (x 10,000)
2.0 mg/l: 2; 7; 23; 96 (x 10,000)
3.6 mg/l: 2; 6; 21; 84 (x 10,000)
6.0 mg/l: 2; 5; 14; 52 (x 10,000)
10.0 mg/l: 2; 5; 6; 17 (x 10,000)
17.0 mg/l: 2; 3; 3; 6 (x 10,000)
Description of key information
The growth inhibition on the freshwater alga Desmodesmus subspicatus was tested by Scholz (1994) over a period of 72 h according to accepted guidance documents (OECD guideline 201). Algae were exposed to 6 concentrations between 1.2 mg/L and 17 mg/L and one control. Since the analytical results indicate the stability of the test item, the results are based on the nominal test item concentrations. Based on the growth rate, the ErC50 of 11 mg/L and a 72 h ErC10 of 5.3 mg/L were determined. Based on biomass development, the EbC50 of 7 mg/L and a 72 h EbC50 of 3.8 mg/L were determined. The NOEC based on cell growth (biomass) was assessed to be 3.6 mg/L.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 11 mg/L
- EC10 or NOEC for freshwater algae:
- 5.3 mg/L
Additional information
Based on biomass development an EbC50 (72 h) of 7.0 mg/L and a 72 h-EbC10 of 3.8 mg/L were determined. Based on growth rate an ErC50 of 11.0 mg/l and a 72 h-ErC10 of 5.3 mg/l were determined. The NOEC based on cell growth (biomass) was assessed to be 3.6 mg/l.
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