1,2,3,4-tetrahydronaphthalene

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference 1

Reference Type:

publication

Title:

Range-finding toxicity data: list IV

Author:

Smyth HF Jr, Carpenter CP and Weil CS

Year:

1951

Bibliographic source:

Arch. Ind. Hyg. Occup. Med. 4, 119-122

Reference 2

Reference Type:

study report

Title:

Unnamed

Year:

1949

Report date:

1949

Materials and methods

Principles of method if other than guideline:

Smyth HF Jr, Carpenter CP (1944): The place of the range finding test in the industrial toxicology laboratory. J. Ind. Hyg. Toxicol. 26, 269-273 and subsequent updates

GLP compliance:

no

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

1,2,3,4-tetrahydronaphthalene

EC Number:

204-340-2

EC Name:

1,2,3,4-tetrahydronaphthalene

Cas Number:

119-64-2

Molecular formula:

C10H12

IUPAC Name:

1,2,3,4-tetrahydronaphthalene

Details on test material:

Tetrahydronaphthalene procured from Eastman Kodak Co. under their number P550 on 10 Jan. 1949. No data on purity

Test animals

Species:

rabbit

Strain:

not specified

Sex:

male

Details on test animals or test system and environmental conditions:

TEST ORGANISMS: 
-male Albino Rabbbits 
-Weight at study initiation: 2,202-2,718 g

Administration / exposure

Type of coverage:

semiocclusive

Vehicle:

other: undiluted

Details on dermal exposure:

ADMINISTRATION: 
- Area covered: clipped trunk
- Occlusion: impervious flexible film ("vinylite" sheeting)
- Exposure period: 24 hours 
- Observation period: 14 days

Duration of exposure:

24 hours

Doses:

12.6; 15.8; 20.0 g/kg bw

No. of animals per sex per dose:

5

Control animals:

no

Statistics:

Thompson, W.R. (1947): Use of moving averages and  interpolation to estimate median effective dose. Bact. Rev. 11, 115

Results and discussion

Effect levels

Key result

Sex:

male

Dose descriptor:

LD50

Effect level:

ca. 16 800 mg/kg bw

Mortality:

MORTALITY:   
 LD50 = 17.3 (14.5 - 20.6) mL/kg bw
    = 16.8 (14.1 - 20.0)  g/kg bw

Clinical signs:

other: CLINICAL SIGNS:  Upon removal of the covering the skin was erythematous,  on subsequent examination it was necrosed and ultimately leathery and dry.

Gross pathology:

Autopsy revealed pale livers and kidneys and congestion of the pancreas and intestines.

Any other information on results incl. tables

- Number of deaths at each dose: 
  12,600 mL/kg bw:  1/5 on day 4
  15,800 mL/kg bw:  1/5 on day 13
  20,000 mL/kg bw:  1+2+1/5 on days 11, 12, 14

Applicant's summary and conclusion

Conclusions:

The LD50 (rabbit) for tetrahydronaphthalene applied to the skin of the rabbit is 17.3 mL/kg bw (=16.8 g/kg bw). Thus tetrahydronaphthalene showed low acute toxicity in rabbits after dermal application.

Executive summary:

The LD50 for tetrahydronaphthalene applied undiluted to the clipped skin of the rabbit trunk under "Vinylit" sheeting for 24h was 17.3 mL/kg bw (14.5 -20.6). Upon removal of the covering the the skin was erythemous, on subsequent examination it was necrosed and ultimately because leathery and dry. Autopsy of victims revealed pale livers, and kidneys and congestion of the pancreas and intestines. The disparity between oral and percutaneous toxicity indicates that tetrahydronaphthalene pentrates quite slowly through the skin.