Registration Dossier
Registration Dossier
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 204-340-2 | CAS number: 119-64-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in mammalian cells
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1991-06-03 to 1992-05-21
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Comparable to guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 992
Materials and methods
- Principles of method if other than guideline:
- Mouse lymphoma assay, suspension plate, no further details
- GLP compliance:
- yes
- Type of assay:
- mammalian cell gene mutation assay
Test material
- Reference substance name:
- 1,2,3,4-tetrahydronaphthalene
- EC Number:
- 204-340-2
- EC Name:
- 1,2,3,4-tetrahydronaphthalene
- Cas Number:
- 119-64-2
- Molecular formula:
- C10H12
- IUPAC Name:
- 1,2,3,4-tetrahydronaphthalene
- Details on test material:
- 1,2,3,4-tetrahydronaphthalene from Chem Service Inc. (West Chester, Pennsylvania, USA), purity 98.2 %
Constituent 1
Method
- Target gene:
- tymidin kinase gene
Species / strain
- Species / strain / cell type:
- mouse lymphoma L5178Y cells
- Metabolic activation:
- with and without
- Metabolic activation system:
- Aroclor 1254-induced rat liver S9 mix
- Test concentrations with justification for top dose:
- -S9: 30-50 and 35-47.5 µg/ml;
+S9: 1-15 and 10-20 µg/ml - Vehicle / solvent:
- DMSO
Controls
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- ethylmethanesulphonate
- Remarks:
- 3-methylcholanthrene with S9-mix Migrated to IUCLID6: without S9-mix
- Details on test system and experimental conditions:
- Mouse lymphoma assay
Metabolic activation system: Aroclor 1254-induced rat liver S9 mix
ADMINISTRATION:
- Dosing:
-S9: 30, 35, 40, 45, 50 µg/ml
+S9: 1, 2.5, 5, 10, 15 µg/ml
follow-up -S9: 35, 40, 42.5, 45, 47.5 µg/ml
follow-up +S9: 10, 12.5, 15, 17.5, 20 µg/ml
solvent: DMSO
- Number of replicates: 2
- Positive and negative control groups and treatment:
positive: ethylmethanesulfonate (-S9); 3-methylcholanthrene (+S9)
negative: solvent and untreated
- Pre-incubation time: two-day expression period - Evaluation criteria:
- CRITERIA FOR EVALUATING RESULTS: dose-dependent increase in mutant frequency
Results and discussion
Test results
- Species / strain:
- mouse lymphoma L5178Y cells
- Metabolic activation:
- with and without
- Genotoxicity:
- ambiguous
- Cytotoxicity / choice of top concentrations:
- other: see Results
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
- GENOTOXIC EFFECTS: Initial study:
- positive with and without metabolic activation
- validity questionable due to findings in controls
Follow-up study:
- negative without metabolic activation
- equivocal with metabolic activation (the only cultures exhibiting a significant increase in mutant frequency had less than 10% total growth)
Controls: Positive controls were as expected in both studies.
Negative controls showed unacceptably high mutant frequencies in the initial study and were as expected in the follow-up study.
CYTOTOXIC CONCENTRATION:
- Toxicity screening study: Complete toxicity >= 100 µg/ml, almost complete at 50 µg/ml (-S9); complete toxicity >= 50 µg/ml (+S9)
- Initial study: "too toxic to clone" at 52 µg/ml (-S9) and 20 µg/ml (+S9)
- Follow-up study: "too toxic to clone" at 50 µg/ml (-S9) and 22.5 µg/ml (+S9) - Remarks on result:
- other: other: L5178Y TK+/-
- Remarks:
- Migrated from field 'Test system'.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
In a mouse lymphoma test tetrahydronaphthalene showed no relevant genotoxic effects. - Executive summary:
In a mouse lymphoma test, a negative result was obtained in the absence of a metabolic activation system (dose range 35-47.5 μg/ml), while with the addition of S-9 mix the test was equivocal (dose range 10-20 μg/ml): The only cultures exhibiting a significant increase in mutant frequency had less than 10 % total growth.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
