Registration Dossier

Data platform availability banner - registered substances factsheets

Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Long-term toxicity to aquatic invertebrates

Currently viewing:

Administrative data

Endpoint:
long-term toxicity to aquatic invertebrates
Type of information:
migrated information: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
disregarded due to major methodological deficiencies
Reliability:
3 (not reliable)
Rationale for reliability incl. deficiencies:
other: Guideline study, however DMSO was used as solubliser

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1994

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: OECD guideline 202
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Calcium 3-hydroxy-4-[(4-methyl-2-sulphonatophenyl)azo]-2-naphthoate
EC Number:
226-109-5
EC Name:
Calcium 3-hydroxy-4-[(4-methyl-2-sulphonatophenyl)azo]-2-naphthoate
Cas Number:
5281-04-9
Molecular formula:
C18H14N2O6S.Ca
IUPAC Name:
calcium 3-hydroxy-4-[(4-methyl-2-sulfonatophenyl)diazenyl]-2-naphthoate
Test material form:
solid: nanoform
Details on test material:
Test materials used in this dossier are all considered to fall under the definition of nano-materials according to the European Commission Recommendation 2011/696/EU as the synthesis and manufacturing of this pigment always yields particulate material with a fine particle size distribution.

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
yes
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): DMSO

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Feeding during test: daily
- Food type: green algae

Study design

Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
21 d

Test conditions

Test temperature:
20 - 22 ± °1C
Nominal and measured concentrations:
Nominal test concentrations: 3-300 mg/l, control of DMSO:HCO-40= 9:1 (100 mg/kg) and laboratory water control.
Details on test conditions:
TEST SYSTEM
- Test vessel: beaker (500 ml)
- Fill volume: 400 ml
- Renewal rate of test solution (frequency/flow rate): renewal of test substance every 48 hours.
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- No. of vessels per vehicle control (replicates): 4
- Biomass loading rate:

OTHER TEST CONDITIONS
- Photoperiod: 8:16 h day night regime

VEHICLE CONTROL PERFORMED: yes

Results and discussion

Effect concentrationsopen allclose all
Duration:
14 d
Dose descriptor:
EC50
Effect conc.:
4.4 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
reproduction
Remarks on result:
other: The test is classified as disregarded study since DMSO was used as solubiliser. DMSO is known to increase the toxicity of substances.
Duration:
21 d
Dose descriptor:
EC50
Effect conc.:
9.1 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
reproduction
Remarks on result:
other: The test is classified as disregarded study since DMSO was used as solubiliser. DMSO is known to increase the toxicity of substances.
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
3 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: mobility and reproduction
Remarks on result:
other: The test is classified as disregarded study since DMSO was used as solubiliser. DMSO is known to increase the toxicity of substances.
Duration:
21 d
Dose descriptor:
LOEC
Effect conc.:
9.4 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: mortality and reproduction
Remarks on result:
other: The test is classified as disregarded study since DMSO was used as solubiliser. DMSO is known to increase the toxicity of substances.

Applicant's summary and conclusion