Reaction mass of 1-(2,6,6-trimethylcyclohex-2-en-1-yl)hepta-1,6-dien-3-one and 1-(2,6,6-trimethylcyclohex-1-en-1-yl)hepta-1,6-dien-3-one

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1955

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Remarks:

Study was performed predating current guidelines

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1955

Report date:

1955

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

Deviations:

yes

Remarks:

5 day observation period

GLP compliance:

no

Test type:

standard acute method

Limit test:

no

Test material

Test material information

1

Reference substance name:

Reaction mass of 1-(2,6,6-trimethylcyclohex-2-en-1-yl)hepta-1,6-dien-3-one and 1-(2,6,6-trimethylcyclohex-1-en-1-yl)hepta-1,6-dien-3-one

EC Number:

904-551-6

Molecular formula:

C16H24O

IUPAC Name:

Reaction mass of 1-(2,6,6-trimethylcyclohex-2-en-1-yl)hepta-1,6-dien-3-one and 1-(2,6,6-trimethylcyclohex-1-en-1-yl)hepta-1,6-dien-3-one

Test material form:

liquid

Test animals

Species:

mouse

Strain:

CF-1

Sex:

not specified

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Carworth
- Housing: Individual cages
- Diet: Regular diet of Fox Blox

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

other: Distilled water plus Tween 20 to aid in mixing

Details on oral exposure:

VEHICLE
- Actual amount of test material fed: 0.1, 0.14, 0.16, 0.18, 0.20, 0.30, and 0.40 mL corresponding with 5, 7, 8, 9, 10, 15, and 20 mL/kg, respectively.
- Justification for choice of vehicle: distilled water and Tween 20 to aid mixing.

DOSAGE PREPARATION
The test material was diluted one part plus four parts with diluted water plus Tween 20.

Doses:

5, 7, 8, 9, 10, 15, and 20 mL/kg

No. of animals per sex per dose:

5, 5, 10, 10, 10,10, and 5 animals (sex unspecified) in the 5, 7, 8, 9, 10, 15, and 20 mL/kg dose goups, respectively.

Control animals:

no

Details on study design:

- Duration of observation period following administration: 5 days

Results and discussion

Effect levels

Key result

Sex:

not specified

Dose descriptor:

LD50

Effect level:

9 500 mL/kg bw

Based on:

test mat.

Remarks on result:

other: LD50 calculated with Behrens method

Mortality:

0/5, 0/5, 1/10, 4/10, 7/10, 9/10, and 5/5 animals died within the 5 day observation period after exposure to 5, 7, 8, 9, 10, 15, and 20 mL/kg, respectively.

Applicant's summary and conclusion

Interpretation of results:

other: Not classified

Remarks:

According to Regulation (EC) No. 1272/2008.

Conclusions:

The acute oral toxicity test showed an LD50 of 9500 mL/kg bw

Executive summary:

A preguideline study was performed to identify the acute oral toxicity of the test substance in mice. In this study, 5, 5, 10, 10, 10,10, and 5 mice (sex unspecified) were administered with 5, 7, 8, 9, 10, 15, and 20 mL/kg . substance at dose levels of 5, 7, 8, 9, 10, 15, and 20 mL/kg bw. 0/5, 0/5, 1/10, 4/10, 7/10, 9/10, and 5/5 animals died within the 5 day observation period after exposure to 5, 7, 8, 9, 10, 15, and 20 mL/kg, respectively. Under the conditions of the test, the acute oral LD50 for the substance in mice was determined to be 9500 mL/kg bw (calculated with Behrens method).