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Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
Test start date: 19 March, 1990; Test end date: 23 March, 1990; Study completion date; 30 May 1990.
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
EU Method C.1 (Acute Toxicity for Fish)
Deviations:
yes
Remarks:
Holding : in dechlorinated tap water; Test : in dechlorinated tap water; Temperature: higher temperatur variation
GLP compliance:
yes
Specific details on test material used for the study:
Stability: guaranteed by the sponsor
Solubility: 50 g/L
Storage: Room temperature
Appearance: Solid


Analytical monitoring:
yes
Details on sampling:
Water samples were taken from the bath at the beginning and at the end of the experiment.
Vehicle:
no
Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
Test organism: Zebrafish (Brachidanio rerio)
Weight: 0.20 g
Length: 28.3 mm
Age: 92 days
Acclimation: 12 days in dechlorinated tap water
Supplier: West Aquarium, Bad Lauterberg, BRD
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Test temperature:
21.3 to 23.6 °C
pH:
8.1
Dissolved oxygen:
6.7 - 7
Nominal and measured concentrations:
1, 1.78, 3.2, 5.6 and 10 mg/L
Details on test conditions:
Vessels: 5 liter glass aquaria
Volume : 2.9 litre (3.0 litre minus 2x 0,05 litre for analytical control)
Diluent: In dechlorinated tap water
Mode of preparation: Appropriate amounts of the Stocksolution were transfered to the fish bath.
Number of fish: 10 fish per concentration and water control
Feeding: None
Aeration: Slowly aerated
System: Static-system
Temperature: 21.3 - 23.6 °C
Duration: 96h
Lighting: 12 hours light / 12 hours darkness (yellow light TL 16/20 W)
Reference substance (positive control):
not specified
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
3.6 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Remarks on result:
other: Confidence limits: 2.87 - 4.09
Details on results:
At 96h: 0/10, 0/10, 0/10, 2/10 10/10 fish were found dead at concentrations of 0 (blank), 1, 1.78, 3.2, 5.6 and 10 mg/L respectively.

ABNORMAL RESPONSES OF THE FISH: At this concentrations tested a change in the swimming behaviour of the test fishes could be observed.
Validity criteria fulfilled:
not specified
Conclusions:
The 96 h LC50 of FAT 31064/F in this fish acute toxicity test was determined to be 3.6 mg/L.
Executive summary:

The acute fish toxicity test was performed according to a methodology of OECD Guideline 203 following GLP. Zebrafish: (Danio rerio) were used in the experiment setup. 10 fish were tested at these different following concentrations: 0, 1, 1.78, 3.2, 5.6 and 10  mg/L. The LC50 - values are calculated with the Spearman-Kärber Method and the Geometric mean. After 96h exposure, 0/10, 0/10, 0/10, 2/10 10/10 fish were found dead at concentrations of 0 (blank), 1, 1.78, 3.2, 5.6 and 10 mg/L respectively. Based on these findings, the LC50 obtained at 96h was 3.6 mg/L.

Description of key information

In 1990 a key acute fish toxicity test was performed according to a methodology of OECD Guideline 203 following GLP.Zebrafish (Danio rerio) 10 in number per concentration were used in the experiment setup. Fish were tested at these different following concentrations: 1.0, 1.78, 3.2, 5.6, 10.0  mg/l. In conclusion the results LC50 obtained at 96h was 3.6 mg/L. Several suppost studies performed during 1978 and 1980 reported 96 h LC50 as 16 and 15 mg/L for Zebra and Forellen fish respectively.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Effect concentration:
3.2 mg/L

Additional information