Reaction mass of 6-Chloro-2-{4[ethyl(2-hydroxyethyl)amino]phenyl}-1-methylbenzo[cd]indolium hydroxide and 6-chloro-2-[4-[ethyl(2-hydroxyethyl)amino]phenyl]-1-methylbenz[cd]indolium acetate

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Test start date: 19 March, 1990; Test end date: 23 March, 1990; Study completion date; 30 May 1990.

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Deviations:

yes

Remarks:

Holding : in dechlorinated tap water; Test : in dechlorinated tap water; Temperature: higher temperatur variation

GLP compliance:

yes

Specific details on test material used for the study:

Stability: guaranteed by the sponsor
Solubility: 50 g/L
Storage: Room temperature
Appearance: Solid

Analytical monitoring:

yes

Details on sampling:

Water samples were taken from the bath at the beginning and at the end of the experiment.

Vehicle:

no

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

Test organism: Zebrafish (Brachidanio rerio)
Weight: 0.20 g
Length: 28.3 mm
Age: 92 days
Acclimation: 12 days in dechlorinated tap water
Supplier: West Aquarium, Bad Lauterberg, BRD

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test temperature:

21.3 to 23.6 °C

pH:

8.1

Dissolved oxygen:

6.7 - 7

Nominal and measured concentrations:

1, 1.78, 3.2, 5.6 and 10 mg/L

Details on test conditions:

Vessels: 5 liter glass aquaria
Volume : 2.9 litre (3.0 litre minus 2x 0,05 litre for analytical control)
Diluent: In dechlorinated tap water
Mode of preparation: Appropriate amounts of the Stocksolution were transfered to the fish bath.
Number of fish: 10 fish per concentration and water control
Feeding: None
Aeration: Slowly aerated
System: Static-system
Temperature: 21.3 - 23.6 °C
Duration: 96h
Lighting: 12 hours light / 12 hours darkness (yellow light TL 16/20 W)

Reference substance (positive control):

not specified

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

3.6 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: Confidence limits: 2.87 - 4.09

Details on results:

At 96h: 0/10, 0/10, 0/10, 2/10 10/10 fish were found dead at concentrations of 0 (blank), 1, 1.78, 3.2, 5.6 and 10 mg/L respectively.

ABNORMAL RESPONSES OF THE FISH: At this concentrations tested a change in the swimming behaviour of the test fishes could be observed.

Validity criteria fulfilled:

not specified

Conclusions:

The 96 h LC50 of FAT 31064/F in this fish acute toxicity test was determined to be 3.6 mg/L.

Executive summary:

The acute fish toxicity test was performed according to a methodology of OECD Guideline 203 following GLP. Zebrafish: (Danio rerio) were used in the experiment setup. 10 fish were tested at these different following concentrations: 0, 1, 1.78, 3.2, 5.6 and 10  mg/L. The LC50 - values are calculated with the Spearman-Kärber Method and the Geometric mean. After 96h exposure, 0/10, 0/10, 0/10, 2/10 10/10 fish were found dead at concentrations of 0 (blank), 1, 1.78, 3.2, 5.6 and 10 mg/L respectively. Based on these findings, the LC50 obtained at 96h was 3.6 mg/L.

Description of key information

In 1990 a key acute fish toxicity test was performed according to a methodology of OECD Guideline 203 following GLP.Zebrafish (Danio rerio) 10 in number per concentration were used in the experiment setup. Fish were tested at these different following concentrations: 1.0, 1.78, 3.2, 5.6, 10.0  mg/l. In conclusion the results LC50 obtained at 96h was 3.6 mg/L. Several suppost studies performed during 1978 and 1980 reported 96 h LC50 as 16 and 15 mg/L for Zebra and Forellen fish respectively.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

3.2 mg/L