Reaction mass of 1-chlorodecane and 1-chlorotetraceane

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1995-11-03 to 1996-02-06

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1996

Report date:

1996

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

other: 92/69/EWG

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

part 1 (1984)

Deviations:

no

Test guideline 3

Qualifier:

according to guideline

Guideline:

other: DIN 38412 part 1

Version / remarks:

national guideline DIN 38412 part 1

Deviations:

not specified

Principles of method if other than guideline:

Method: other: 92/69/EWG

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

1-chlorotetradecane

EC Number:

219-368-0

EC Name:

1-chlorotetradecane

Cas Number:

2425-54-9

Molecular formula:

C14H29Cl

IUPAC Name:

1-chlorotetradecane

Test material form:

liquid

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

The stability of test substance was tested by GC analysis before test begin. The concentration of the stock solution was analysed at test begin and test end.

Test solutions

Vehicle:

no

Details on test solutions:

1g/L of the test substance was dissolved in synthetic freshwater and stirred for 18 h. The solution was filtered and the test substance concentration was analysed.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: water flea
- Strain/clone: Daphnia magna straus, Klon 5
- Source: own cultivation
- Feeding during test: no
24 h before test start the parental daphnids were removed, the juveniles (newly hatched overnight) are used in the test.

ACCLIMATION
- Acclimation conditions: M4 Elendt medium
- Type and amount of food: ad libitum
- Feeding frequency: daily
- Health during acclimation (any mortality observed): healthy stock

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Test temperature:

20 +/- 1 °C

pH:

7.8 to 7.9 at the end of test

Dissolved oxygen:

7.6 to 8.2 mg O2/L at test end

Nominal and measured concentrations:

0.0078 mg/L, 0.013 mg/L, 0.022 mg/L, 0.039 mg/L, 0.065 mg/L, 0.117 mg/L (geometric mean of the stock solution 0 h - 48 h)

Details on test conditions:

TEST SYSTEM
- Test vessel: circular vessels with 10 mL test solution
- Aeration: no
- No. of organisms per vessel: 5 daphnids/ vessel
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 1
- Biomass loading rate: ~2 mL per daphnid

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: synthetic freshwater (with Ca 2+ and Mg 2+ ions of 2.5 mmol and a ratio of 4:1; ratio of Na+ to K+ ions 10:1)
- Adjustment of pH: no
- Photoperiod: darkness

EFFECT PARAMETERS MEASURED: (im)mobility after 24 h and 48 h

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

0.11 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other:

Remarks:

conf. interval 0.0065 mg/L - 0.12 mg/L

Effect concentrations 2

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

0.065 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

> 0.12 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 4

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 0.12 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

The test substance concentration was found to be not stable over the test period (test substance concentration decreased >20-50%). Therefore a geometric mean of the concentration of stock solution was determined. The test concentrations were calculated from the stock solution.

Results with reference substance (positive control):

Tested concentrations: 1.0 mg/L and 2.0 mg/L. After 24 h 30% of the daphnids were immobilized in the test concentration of 1.0 mg/L, and 100 % were immobilized with a test concentration of 2.0 mg/L. The sensitivity of the daphnid is in the normal range and the test is considered to be valid.

Applicant's summary and conclusion

Validity criteria fulfilled:

not specified

Conclusions:

The EC50 value after 48 h exposure is 0.11 mg/L. After exposure for 48 h, the highest concentration at which no immobilization (~ 10 %) occurred was, 0.065 mg/L. After exposure for 48 h, the lowest concentration at which 100 % immobilization occurred was, > 0.12 mg/L (the highest concentrations are based on the substance). The highest achieved concentration under the test coditions is 0.12 mg/L (geometric mean). All concentrations are based on the substance.