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Diss Factsheets

Environmental fate & pathways

Biodegradation in water: screening tests

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Administrative data

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biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
weight of evidence
Study period:
4 (not assignable)
Rationale for reliability incl. deficiencies:
other: Study conducted according to OECD Guideline 301 C but with deviations: no detail on material and methods
Justification for type of information:
Read-across based on results from MITI data on beta-myrcene extrapolated to beta-ocimene.
Indeed, beta-myrcene and beta-ocimene have similar SMILES and the same Molecular Weight (136.24 g/mol) as well as the same formula (C10 H16). In addition, their chemical class is neutral organics for the both substances according to EPISuite ECOSAR v1.11. Furthermore, their log Kow and solubility have been estimated with EPISuite Kowwin v1.68 and EPISuite WSKowwin v 1.43 respectively. The log Kow and Water Solubility Estimates were in the same order (Log Kow = 4.17; WS = 9.02 mg/L and Log Kow = 4.80; WS = 2.01 mg/L for beta-myrcene and for beta-ocimene respectively. Based on all this information, it can be assumed that having similar properties and mode of action, similar environmental behaviour is expected. That is why ready biodegradation results from beta-myrcene is here extrapolated to beta-ocimene.
according to guideline
OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))
no detail on material and methods
Principles of method if other than guideline:
Not applicable
GLP compliance:
not specified
Specific details on test material used for the study:
No data
Oxygen conditions:
Inoculum or test system:
activated sludge (adaptation not specified)
Details on inoculum:
- Concentration of sludge: 30 ppm
Duration of test (contact time):
2 wk
Initial conc.:
100 other: ppm
Based on:
test mat.
Details on study design:
No data
Preliminary study:
No data
Test performance:
No data
% degradation (O2 consumption)
Sampling time:
2 wk
Details on results:
- Indirect analysis (BOD): 86%
- Direct analysis (GC): 99%
- Direct analysis (TOC): 87%
Results with reference substance:
No data


Validity criteria fulfilled:
not specified
Interpretation of results:
readily biodegradable
Under the test conditions, myrcene beta was readily biodegradable.
Executive summary:

In a ready biodegradation study performed according to OECD Guideline 301 C, myrcene-beta was tested at concentrations of 100 ppm and the inoculum was activated sludge (30 ppm). The degradation of the test material was assessed by the determination of the oxygen consumption.

At 100 ppm test concentration, 86% degradation by biochemical oxygen demand was reached in 2 weeks.


Under the test conditions, myrcene beta was readily biodegradable.


Description of key information

Based on a read-across from an analogue substance (myrcene), beta-ocimene can be regarded as readily biodegradable.

Key value for chemical safety assessment

Biodegradation in water:
readily biodegradable

Additional information