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Environmental fate & pathways

Biodegradation in water: screening tests

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Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference
Reference Type:
publication
Title:
Structural Determinants in the Oxidation of Aliphatic Compounds by Activated Sludge
Author:
Malaney, G.W.; Gerhold, R.M.
Year:
1969
Bibliographic source:
J. Wat. Pollut. Control. Fed. Vol 41, R18-R33

Materials and methods

Principles of method if other than guideline:
Scientific study by using activated sludge in a Warburg manometer.
The study investigated the ability of activated sludges from 3 municipal waste treatment plants in the Columbus, Ohio, area to oxidise i.a. L-4-hydroxyproline. The experimental design was based upon exposure of i.a. L-4-hydroxyproline at a concentration of 500 mg/l to activated sludge solids at 2500 mg/l in the Warburg respiriometer with oxygen uptake as the measure of oxidation of the test compound.
GLP compliance:
no
Remarks:
Study performed prior to implementation of GLP.

Test material

Constituent 1
Chemical structure
Reference substance name:
L-4-hydroxyproline
EC Number:
200-091-9
EC Name:
L-4-hydroxyproline
Cas Number:
51-35-4
Molecular formula:
C5H9NO3
IUPAC Name:
L-4-hydroxyproline
Test material form:
solid: crystalline

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, industrial (adaptation not specified)
Duration of test (contact time):
24 h
Initial test substance concentration
Initial conc.:
500 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
O2 consumption
Reference substance
Reference substance:
not required

Results and discussion

Preliminary study:
Not applicable
Test performance:
Not applicable
% Degradationopen allclose all
Parameter:
other: Percentage of Theoretical Oxygen Demand
Value:
1
Sampling time:
6 h
Parameter:
other: Percentage of Theoretical Oxygen Demand
Value:
2.9
Sampling time:
12 h
Key result
Parameter:
other: Percentage of Theoretical Oxygen Demand
Value:
18.2
Sampling time:
24 h
Details on results:
The study investigated the ability of activated sludges from 3 municipal waste treatment plants in the Columbus, Ohio, area to oxidise i.a. L-4-hydroxyproline. The experimental design was based upon exposure of i.a. L-4-hydroxyproline at a concentration of 500 mg/l to activated sludge solids at 2500 mg/l in the Warburg respiriometer with oxygen uptake as the measure of oxidation of the test compound.

BOD5 / COD results

BOD5 / COD
Remarks on result:
other: TOD was measured.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Interpretation of results:
readily biodegradable
Conclusions:
L-4-hydroxyproline is rapidly oxidized in activated sludge. After only 24 h already more than 18 % of the Theoretical Oxygen Demand were achieved. This leads to the conclusion that the test substance is readily biodegradable.
Executive summary:

L-4-hydroxyproline is considered readily biodegradable.