Registration Dossier
Registration Dossier
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EC number: 200-091-9 | CAS number: 51-35-4
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
Data source
Reference
- Reference Type:
- publication
- Title:
- Structural Determinants in the Oxidation of Aliphatic Compounds by Activated Sludge
- Author:
- Malaney, G.W.; Gerhold, R.M.
- Year:
- 1 969
- Bibliographic source:
- J. Wat. Pollut. Control. Fed. Vol 41, R18-R33
Materials and methods
- Principles of method if other than guideline:
- Scientific study by using activated sludge in a Warburg manometer.
The study investigated the ability of activated sludges from 3 municipal waste treatment plants in the Columbus, Ohio, area to oxidise i.a. L-4-hydroxyproline. The experimental design was based upon exposure of i.a. L-4-hydroxyproline at a concentration of 500 mg/l to activated sludge solids at 2500 mg/l in the Warburg respiriometer with oxygen uptake as the measure of oxidation of the test compound. - GLP compliance:
- no
- Remarks:
- Study performed prior to implementation of GLP.
Test material
- Reference substance name:
- L-4-hydroxyproline
- EC Number:
- 200-091-9
- EC Name:
- L-4-hydroxyproline
- Cas Number:
- 51-35-4
- Molecular formula:
- C5H9NO3
- IUPAC Name:
- L-4-hydroxyproline
- Test material form:
- solid: crystalline
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, industrial (adaptation not specified)
- Duration of test (contact time):
- 24 h
Initial test substance concentration
- Initial conc.:
- 500 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- O2 consumption
Reference substance
- Reference substance:
- not required
Results and discussion
- Preliminary study:
- Not applicable
- Test performance:
- Not applicable
% Degradationopen allclose all
- Parameter:
- other: Percentage of Theoretical Oxygen Demand
- Value:
- 1
- Sampling time:
- 6 h
- Parameter:
- other: Percentage of Theoretical Oxygen Demand
- Value:
- 2.9
- Sampling time:
- 12 h
- Key result
- Parameter:
- other: Percentage of Theoretical Oxygen Demand
- Value:
- 18.2
- Sampling time:
- 24 h
- Details on results:
- The study investigated the ability of activated sludges from 3 municipal waste treatment plants in the Columbus, Ohio, area to oxidise i.a. L-4-hydroxyproline. The experimental design was based upon exposure of i.a. L-4-hydroxyproline at a concentration of 500 mg/l to activated sludge solids at 2500 mg/l in the Warburg respiriometer with oxygen uptake as the measure of oxidation of the test compound.
BOD5 / COD results
BOD5 / COD
- Remarks on result:
- other: TOD was measured.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- L-4-hydroxyproline is rapidly oxidized in activated sludge. After only 24 h already more than 18 % of the Theoretical Oxygen Demand were achieved. This leads to the conclusion that the test substance is readily biodegradable.
- Executive summary:
L-4-hydroxyproline is considered readily biodegradable.
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