L-4-hydroxyproline

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference Type:

publication

Title:

Structural Determinants in the Oxidation of Aliphatic Compounds by Activated Sludge

Author:

Malaney, G.W.; Gerhold, R.M.

Year:

1969

Bibliographic source:

J. Wat. Pollut. Control. Fed. Vol 41, R18-R33

Materials and methods

Principles of method if other than guideline:

Scientific study by using activated sludge in a Warburg manometer.
The study investigated the ability of activated sludges from 3 municipal waste treatment plants in the Columbus, Ohio, area to oxidise i.a. L-4-hydroxyproline. The experimental design was based upon exposure of i.a. L-4-hydroxyproline at a concentration of 500 mg/l to activated sludge solids at 2500 mg/l in the Warburg respiriometer with oxygen uptake as the measure of oxidation of the test compound.

GLP compliance:

no

Remarks:

Study performed prior to implementation of GLP.

Test material

Test material information

Constituent 1

Reference substance name:

L-4-hydroxyproline

EC Number:

200-091-9

EC Name:

L-4-hydroxyproline

Cas Number:

51-35-4

Molecular formula:

C5H9NO3

IUPAC Name:

L-4-hydroxyproline

Test material form:

solid: crystalline

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, industrial (adaptation not specified)

Duration of test (contact time):

24 h

Initial test substance concentration

Initial conc.:

500 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

O2 consumption

Reference substance

Reference substance:

not required

Results and discussion

Preliminary study:

Not applicable

Test performance:

Not applicable

% Degradation

% Degradation 1

Parameter:

other: Percentage of Theoretical Oxygen Demand

Value:

1

Sampling time:

6 h

% Degradation 2

Parameter:

other: Percentage of Theoretical Oxygen Demand

Value:

2.9

Sampling time:

12 h

% Degradation 3

Key result

Parameter:

other: Percentage of Theoretical Oxygen Demand

Value:

18.2

Sampling time:

24 h

Details on results:

The study investigated the ability of activated sludges from 3 municipal waste treatment plants in the Columbus, Ohio, area to oxidise i.a. L-4-hydroxyproline. The experimental design was based upon exposure of i.a. L-4-hydroxyproline at a concentration of 500 mg/l to activated sludge solids at 2500 mg/l in the Warburg respiriometer with oxygen uptake as the measure of oxidation of the test compound.

BOD5 / COD results

BOD5 / COD

Remarks on result:

other: TOD was measured.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

readily biodegradable

Conclusions:

L-4-hydroxyproline is rapidly oxidized in activated sludge. After only 24 h already more than 18 % of the Theoretical Oxygen Demand were achieved. This leads to the conclusion that the test substance is readily biodegradable.

Executive summary:

L-4-hydroxyproline is considered readily biodegradable.