Registration Dossier
Registration Dossier
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EC number: 274-039-9 | CAS number: 69559-11-1
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 016
- Report date:
- 2016
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
- Deviations:
- yes
- Remarks:
- pH of the test solution in the study was outside the range stipulated for the study (6-9). The pH was 9.2, 9.5 and 9.9 and was not adjusted before starting the test. The authors/sponsors concluded that the pH did not impact the results.
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 2-(4-aminopentyl(ethyl)amino)ethanol
- EC Number:
- 274-039-9
- EC Name:
- 2-(4-aminopentyl(ethyl)amino)ethanol
- Cas Number:
- 69559-11-1
- Molecular formula:
- C9H22N2O
- IUPAC Name:
- 2-[(4-aminopentyl)(ethyl)amino]ethan-1-ol
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Remarks:
- HPLC/MS/MS
Test organisms
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Post exposure observation period:
- Microscopic observation of the algae.
After 24, 48 and 72 hours, algal cells were measured by optical density at 683 nm.
Test conditions
- Hardness:
- Not specified
- Test temperature:
- 22.15 to 24.80°C
- pH:
- 8.0-9.9
- Dissolved oxygen:
- Not specified
- Nominal and measured concentrations:
- Nominal concentrations: 6.25, 12.5, 25, 50, 100 m/L
Measured concentrations: not specified
Nominal concentrations were chosen for the GLP test, according to the results of the preliminary test. - Details on test conditions:
- Continuous light at 5500 lux.
- Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 63.8 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 42 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 12.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 25 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 21.4 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: yield
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 5.26 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: yield
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 6.25 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: yield
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 12.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: yield
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Based on the experimental results for Hydroxu-novol-diamine, the 72-hour ErC50 was 63.8 mg/L and the 72-hour EyC50 was 21.4 mg/L.
The 72-hour NOEC (based on growth inhibition) was 12.5 mg/L and the associated LOEC was 25 mg/L.
The 72-hour NOEC (based on yield inhibition) was 6.25 mg/L and the associated LOEC was 12.5 mg/L.
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