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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2016
Report date:
2016

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
Deviations:
yes
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
2-(4-aminopentyl(ethyl)amino)ethanol
EC Number:
274-039-9
EC Name:
2-(4-aminopentyl(ethyl)amino)ethanol
Cas Number:
69559-11-1
Molecular formula:
C9H22N2O
IUPAC Name:
2-(4-aminopentyl(ethyl)amino)ethanol

Sampling and analysis

Analytical monitoring:
yes
Remarks:
HPLC/MS/MS

Test organisms

Test organisms (species):
Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
72 h
Post exposure observation period:
Microscopic observation of the algae.
After 24, 48 and 72 hours, algal cells were measured by optical density at 683 nm.

Test conditions

Hardness:
Not specified
Test temperature:
22.15 to 24.80°C
pH:
8.0-9.9
Dissolved oxygen:
Not specified
Nominal and measured concentrations:
Nominal concentrations: 6.25, 12.5, 25, 50, 100 m/L
Measured concentrations: not specified
Nominal concentrations were chosen for the GLP test, according to the results of the preliminary test.
Details on test conditions:
Continuous light at 5500 lux.
Reference substance (positive control):
yes
Remarks:
potassium dichromate

Results and discussion

Effect concentrationsopen allclose all
Key result
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
63.8 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Key result
Duration:
72 h
Dose descriptor:
EC10
Effect conc.:
42 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Key result
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
12.5 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Key result
Duration:
72 h
Dose descriptor:
LOEC
Effect conc.:
25 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
21.4 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: yield
Duration:
72 h
Dose descriptor:
EC10
Effect conc.:
5.26 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: yield
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
6.25 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: yield
Duration:
72 h
Dose descriptor:
LOEC
Effect conc.:
12.5 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: yield

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
Based on the experimental results for Hydroxu-novol-diamine, the 72-hour ErC50 was 63.8 mg/L and the 72-hour EyC50 was 21.4 mg/L.
The 72-hour NOEC (based on growth inhibition) was 12.5 mg/L and the associated LOEC was 25 mg/L.
The 72-hour NOEC (based on yield inhibition) was 6.25 mg/L and the associated LOEC was 12.5 mg/L.