4-amino-N-(4-aminophenyl)benzenesulphonamide

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

Data is from study report.

Qualifier:

according to guideline

Guideline:

other: OECD guideline

Principles of method if other than guideline:

Short term toxicity study to Daphnia magna for carried out for 48 hrs according to the OECD guideline.

GLP compliance:

not specified

Specific details on test material used for the study:

- Name of test material : 4-amino-N-(4-aminophenyl)benzene-1-sulfonamide
- Molecular formula : C12H13N3O2S
- Molecular weight : 263.32 g/mol
- Smiles notation : O=S(=O)(c1ccc(cc1)Nc1ccc(cc1)N)N
- InChl : 1S/C12H13N3O2S/c13-9-1-3-10(4-2-9)15-11-5-7-12(8-6-11)18(14,16)17/h1-8,15H,13H2,(H2,14,16,17)
- Substance type : Organic
- Physical state : Solid
- Lot number: 96600
- Solubility in water: 250 mg/l at 323K

Analytical monitoring:

not specified

Details on sampling:

- Concentrations: The concentration of the substance in the stock was 500 mg/l. Nominal test chemical concentration used for the study were 0, 7.81, 15.6, 31.2, 62.5, 125, 250 and 500 mg/l, respectively.
- Sampling method: The stock was stirred for 60 min at 293 kelvin. It was diluted by using dilution water. 10 ml of the appropriate dilution were added per test vessel.

Vehicle:

not specified

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna Straus
- Source: The test organism Daphnia magna Straus was from the Institute National Research Chemical Applied, France.
- Age of parental stock (mean and range, SD): 6-24 h old
- Feeding during test: Yes
- Food type: At water change bewer’s yeast 1X every day green algae culture
- Frequency: 1X

Test type:

static

Water media type:

freshwater

Total exposure duration:

48 h

Hardness:

Total hardness: 2.70 ± 0.50 mmol/l

Test temperature:

292-294 K

pH:

7.7 – 8.3

Dissolved oxygen:

Oxygen content: > 2 mg/l

Conductivity:

550-650 µSiemens/cm

Nominal and measured concentrations:

Nominal concentrations: 0, 7.81, 15.6, 31.2, 62.5, 125, 250 and 500 mg/l

Details on test conditions:

TEST SYSTEM
- Test vessel: centrifuge glass with flattened bottom was used as a test vessel.
- Material, size, headspace, fill volume: 10 ml
- Aeration: The test water is aerated with oxygen free air until it is oxygenated and then allowed to stand for at least another 24 h for stabilization.
- Volume/Tier: 2 ml
- No. of organisms per vessel: 5
- Total no. of animals/conc.: 20
- No. of vessels per concentration (replicates): 4 repilcates

TEST MEDIUM / WATER PARAMETERS
- Ca/mg ratio: ca. 4:1
- Molar ratio Na: K: ca. 10:1
- Conductivity: 550-650 µSiemens/cm
- Ks to pH 4.3: 0.80 ± 0.10 mmol/l

OTHER TEST CONDITIONS
- Light intensity: Day:Night – Rhythm
16: 8 hours
- Illuminance: ca. 5 micro Einstein in wavelength range from 400 – 750 nm

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Floatable animals i.e mobility of the test animal was measured after a period of 24 and 48 hours.

RANGE-FINDING STUDY
- Test concentrations: 0, 7.81, 15.6, 31.2, 62.5, 125, 250 and 500 mg/l (nominal concentrations)

Reference substance (positive control):

not specified

Key result

Duration:

24 h

Dose descriptor:

EC0

Effect conc.:

31.2 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Key result

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

149.12 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other: 95% C. I., = 146.78 – 151.46 mg/l

Key result

Duration:

24 h

Dose descriptor:

EC100

Effect conc.:

> 500 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Key result

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

31.2 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

56.09 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other: 95% C. I. = 50.31 – 61.87 mg/l

Key result

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

250 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Details on results:

At the beginning of the test and after 24 + 48 h, the immobility of the Daphnia used is reduced. The animals are considered to be buoyant after a light swim Do not bump into the test vessel to show any more swimming movements.

Reported statistics and error estimates:

To determine the EC 50 after 24 h, the probit method according to Finney was used.
To determine the EC 50 after 48 h, moving average - method applied.
The EC0 was the highest concentration tested at which an effect of 10% occurred.
EC100 was the lowest tested concentration at 100% effect occurred.

Physical and chemical measurements

Table 1: Test water (values and trial beginning)

pH value: 7.8

Conductivity: 640 µSiemens/cm

Total hardness: 2.42 mmol/l

Ks to pH 4.3: 0.74 mmol/l

Temperature: 297.9 K

Measured on: 1987-07-01

 

Table 2: pH valuein a test flask (parallel 1) per test concentration

Conc. (mg/l)	pH value at
	0 h	48 h
7.81 15.6 31.2 62.5 125. 250. 500. 0.	8.21 8.12 8.09 8.06 8.03 8.00 7.95 8.28	8.16 8.08 8.1 8.05 8.03 8.01 8.01 8.24

Table 3: Oxygen levels in a test flask (parallel 1) per test concentration

Conc. (mg/l)	Oxygen values at
	0 h	48 h
7.81 15.6 31.2 62.5 125. 250. 500. 0.	8.79 8.75 8.76 8.77 8.78 8.65 8.73 8.59	8.6 8.62 8.71 8.81 8.65 8.62 8.83 8.71

 

Table 4: Floatable test animals

 

Conc. (mg/l)			Floatable test animals at
	0 h	24 h		48 h
7.81 15.6 31.2 62.5 125. 250. 500. 0.	20 20 20 20 20 20 20 20	20 20 18 16 16 5 5 20		20 20 18 8 1 0 0 20

Table: Floating animals of the individual in parallel

Conc. (mg/l)	Pa1			Pa2			Pa3			Pa4
	0 h	24 h	48 h	0 h	24 h	48 h	0 h	24 h	48 h	0 h	24 h	48 h
7.81 15.6 31.2 62.5 125. 250. 500. 0.	5 5 5 5 5 5 5 5	5 5 5 3 3 0 0 5	5 5 5 2 0 0 0 5	5 5 5 5 5 5 5 5	5 5 5 3 3 0 1 5	5 5 5 2 0 0 0 5	5 5 5 5 5 5 5 5	5 5 4 5 2 2 1 5	5 5 4 2 0 0 0 5	5 5 5 5 5 5 5 5	5 5 4 3 2 3 3 5	5 5 4 2 1 0 0 5

Validity criteria fulfilled:

not specified

Conclusions:

Based on the effect of the test chemical 4-amino-N-(4-aminophenyl)benzene-1-sulfonamide on the mobility of the test organism Daphnia magna, the 24 hr EC0, EC50 and EC100 value was determined to be 31.2, 149.12 and > 500 mg/l, respectively and 48 hr EC0, EC50 and EC100 value was determined to be 31.2, 56.09 and 250 mg/l, respectively.

Executive summary:

Short term toxicity study to Daphnia magna for carried out for 48 hrs according to the OECD guideline.The study was based on the effects of the test compound 4 -amino-N-(4 -aminophenyl)benzene-1 -sulfonamide(CAS no. 16803 -97 -7) on Daphnia magnain a static fresh water system at a temperature of 292 -294 K, pH range 7.7 – 8.3, hardness of water was 2.70 ± 0.50 mmol/l and oxygen content was > 2 mg/l, respectively. Daphnia magna Straus of 6 -24 h old was used as a test organism obtained from the Institute National Research Chemical Applied, France. Test animals were fed bewer’s yeast 1X every day green algae culture.The concentration of the substance in the stock was 500 mg/l. Nominal test chemical concentration used for the study were 0, 7.81, 15.6, 31.2, 62.5, 125, 250 and 500 mg/l, respectively.Centrifuge glass with flattened bottom was used as a test vessel.The test water used is tap water, which is passed through a 6-micron filter cartridge and through an activated carbon filter to remove any chlorine that may be present.The test water is aerated with oxygen free air until it is oxygenated and then allowed to stand for at least another 24 h for stabilization. No. of organisms used per vessel was 5 and total no. of organisms/conc. were 20, respectively. The test was performed in 4 repilcates with a light intensity of 16: 8 hours Day : Night. Floatable animals i.e mobility of the test animal was measured after a period of 24 and 48 hours.EC50 after 24 and 48 hrs was determined using the statistical probit and moving average method. At the beginning of the test and after 24 + 48 h, the immobility of the Daphnia used is reduced. Based on the effect of the test chemical 4-amino-N-(4-aminophenyl)benzene-1-sulfonamide on the mobility of the test organism Daphnia magna, the24 hr EC0, EC50 and EC100 value was determined to be 31.2, 149.12 and > 500 mg/l, respectively and 48 hr EC0, EC50 and EC100 value was determined to be 31.2, 56.09 and 250 mg/l, respectively.Thus, based on the EC50 value (48 hr = 56.09 mg/l), it can be concluded that the substance 4-amino-N-(4-aminophenyl)benzene-1-sulfonamide can be considered as toxic to aquatic organisms and thus can be considered to be classified in aquatic chronic category 3 as per the CLP classification criteria.

Description of key information

Short term toxicity study to Daphnia magna for carried out for 48 hrs according to the OECD guideline (Sustainability Support Services (Europe) AB, Report no. 1/0164/2/87-164/87, 1987). The study was based on the effects of the test compound 4 -amino-N-(4 -aminophenyl)benzene-1 -sulfonamide(CAS no. 16803 -97 -7) on Daphnia magnain a static fresh water system at a temperature of 292 -294 K, pH range 7.7 – 8.3, hardness of water was 2.70 ± 0.50 mmol/l and oxygen content was > 2 mg/l, respectively. Daphnia magna Straus of 6 -24 h old was used as a test organism obtained from the Institute National Research Chemical Applied, France. Test animals were fed bewer’s yeast 1X every day green algae culture. The concentration of the substance in the stock was 500 mg/l. Nominal test chemical concentration used for the study were 0, 7.81, 15.6, 31.2, 62.5, 125, 250 and 500 mg/l, respectively.Centrifuge glass with flattened bottom was used as a test vessel. The test water used is tap water, which is passed through a 6-micron filter cartridge and through an activated carbon filter to remove any chlorine that may be present. The test water is aerated with oxygen free air until it is oxygenated and then allowed to stand for at least another 24 h for stabilization. No. of organisms used per vessel was 5 and total no. of organisms/conc. were 20, respectively. The test was performed in 4 repilcates with a light intensity of 16: 8 hours Day : Night. Floatable animals i.e mobility of the test animal was measured after a period of 24 and 48 hours.EC50 after 24 and 48 hrs was determined using the statistical probit and moving average method. At the beginning of the test and after 24 + 48 h, the immobility of the Daphnia used is reduced. Based on the effect of the test chemical 4-amino-N-(4-aminophenyl)benzene-1-sulfonamide on the mobility of the test organism Daphnia magna, the24 hr EC0, EC50 and EC100 value was determined to be 31.2, 149.12 and > 500 mg/l, respectively and 48 hr EC0, EC50 and EC100 value was determined to be 31.2, 56.09 and 250 mg/l, respectively. Thus, based on the EC50 value (48 hr = 56.09 mg/l), it can be concluded that the substance 4-amino-N-(4-aminophenyl)benzene-1-sulfonamide can be considered as toxic to aquatic organisms and thus can be considered to be classified in aquatic chronic category 3 as per the CLP classification criteria.

 

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

56.09 mg/L

Additional information

Short term toxicity study to Daphnia magna for carried out for 48 hrs according to the OECD guideline (Sustainability Support Services (Europe) AB, Report no. 1/0164/2/87-164/87, 1987). The study was based on the effects of the test compound 4 -amino-N-(4 -aminophenyl)benzene-1 -sulfonamide(CAS no. 16803 -97 -7) on Daphnia magnain a static fresh water system at a temperature of 292 -294 K, pH range 7.7 – 8.3, hardness of water was 2.70 ± 0.50 mmol/l and oxygen content was > 2 mg/l, respectively. Daphnia magna Straus of 6 -24 h old was used as a test organism obtained from the Institute National Research Chemical Applied, France. Test animals were fed bewer’s yeast 1X every day green algae culture. The concentration of the substance in the stock was 500 mg/l. Nominal test chemical concentration used for the study were 0, 7.81, 15.6, 31.2, 62.5, 125, 250 and 500 mg/l, respectively.Centrifuge glass with flattened bottom was used as a test vessel. The test water used is tap water, which is passed through a 6-micron filter cartridge and through an activated carbon filter to remove any chlorine that may be present. The test water is aerated with oxygen free air until it is oxygenated and then allowed to stand for at least another 24 h for stabilization. No. of organisms used per vessel was 5 and total no. of organisms/conc. were 20, respectively. The test was performed in 4 repilcates with a light intensity of 16: 8 hours Day : Night. Floatable animals i.e mobility of the test animal was measured after a period of 24 and 48 hours.EC50 after 24 and 48 hrs was determined using the statistical probit and moving average method. At the beginning of the test and after 24 + 48 h, the immobility of the Daphnia used is reduced. Based on the effect of the test chemical 4-amino-N-(4-aminophenyl)benzene-1-sulfonamide on the mobility of the test organism Daphnia magna, the24 hr EC0, EC50 and EC100 value was determined to be 31.2, 149.12 and > 500 mg/l, respectively and 48 hr EC0, EC50 and EC100 value was determined to be 31.2, 56.09 and 250 mg/l, respectively. Thus, based on the EC50 value (48 hr = 56.09 mg/l), it can be concluded that the substance 4-amino-N-(4-aminophenyl)benzene-1-sulfonamide can be considered as toxic to aquatic organisms and thus can be considered to be classified in aquatic chronic category 3 as per the CLP classification criteria.