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Diss Factsheets

Toxicological information

Acute Toxicity: inhalation

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Administrative data

Endpoint:
acute toxicity: inhalation
Type of information:
experimental study
Adequacy of study:
key study
Study period:
No data
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Study not conducted according to international guideline, but performed under standardized conditions. No data on whether study was conducted under GLP.
Cross-reference
Reason / purpose for cross-reference:
reference to same study
Reference
Endpoint:
acute toxicity: inhalation
Type of information:
read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Justification for type of information:
See attached justification
Reason / purpose for cross-reference:
read-across source
Preliminary study:
Not relevant
Key result
Sex:
male/female
Dose descriptor:
LC50
Effect level:
> 3.2 mg/L air
Exp. duration:
90 min
Remarks on result:
other: no deaths after exposure to 27 mg linalool in 8.4 litres of air
Mortality:
No data
Clinical signs:
other: The exposure led to a decrease in motility of the mice. The observed motility compared to the motility of the untreated control animals was 32%/96% after 30 minutes inhalation, 8%/85% after 60 minutes inhalation and 0%/71% after 90 minutes inhalation for
Body weight:
No data
Gross pathology:
No data
Other findings:
No data

The concentration of linalool in the blood samples following inhalation was approximately 1, 2.8 and 3 ng/ml after 30 minutes, 60 minutes and 90 minutes of exposure, respectively.

Interpretation of results:
other: causes a decrease in motility in mice
Conclusions:
Linalool caused a decrease in motility in mice in a 90-minute inhalation study. This result was used for read-across to ethyllinalyl acetate.
 
Executive summary:

The influence of linalool on the motility in mice was tested in an inhalation study under standardized conditions. After 30 minutes, 60 minutes and 90 minutes, the motility in the exposed mice was decreased to 32%/96%, 8%/85% and 0%/71% (6 -8 week old mice/6 month old mice) of the motility of untreated control animals. No deaths were reported at the concentration tested (approximately 3.2 mg/l), therefore the LC50 was determined to be >3.2 mg/l. This result was used for read-across to ethyllinalyl acetate.

 

Data source

Reference
Reference Type:
publication
Title:
Aromatherapy: Evidence for Sedative Effects of the Essential Oil of Lavender after Inhalation
Author:
Buchbauer G, Jirovetz L, Jäger W, Dietrich H, Plank C and Karamat E
Year:
1991
Bibliographic source:
Zeitschrift für Naturforschung 46c, 1067-1072

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
Mice were exposed to linalool in air under standardized conditions, and the effects on motility was determined.
GLP compliance:
no
Test type:
other: Inhalation study under standardized conditions
Limit test:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Linalool
EC Number:
201-134-4
EC Name:
Linalool
Cas Number:
78-70-6
Molecular formula:
C10H18O
IUPAC Name:
3,7-dimethylocta-1,6-dien-3-ol
Details on test material:
- Name of test material (as cited in study report): Linalool
- Substance type: fragrance compound
- Obtained from Dragoco-Vienna
No further details provided

Test animals

Species:
mouse
Strain:
Swiss
Sex:
male/female
Details on test animals or test system and environmental conditions:
TEST ANIMALS- Source: no data- Age at study initiation: 6-8 week and 6 months old- Weight at study initiation: 28.5 g- Housing: in groups of 4 on a bedding of wood shavings in polycarbonate cages (Makrolon, type II)- Diet (e.g. ad libitum): standardized pelleted food T 799 (Tagger, Graz, Austria) ad libitum- Water (e.g. ad libitum): ad libitum- Acclimation period: one hour adaptation period was offered to the animals in which no pharmacological treatment occurred.ENVIRONMENTAL CONDITIONS- Temperature (°C): 22 ± 2°C- Humidity (%): 60 ± 10%- Air changes (per hr): 12-15- Photoperiod (hrs dark / hrs light): 12/12

Administration / exposure

Route of administration:
inhalation: vapour
Type of inhalation exposure:
whole body
Remarks:
the substance was introduced directly into the cage in which the mice were present
Vehicle:
other: unchanged (no vehicle)
Details on inhalation exposure:
GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTIONFor the exposure of the animals to the fragrance compounds a small glass tube with a slit measuring 3 mm in width and 5 cm in length was used. The fragrance compounds were injected through a small hole of the cage wall and the rubber plug of the glass tube. Immediately after placing the mice into the cages and the horizontal fixation of the glass tube a transparent plastic seal was fixed at the cage to form an airtight seal. A pumping-evaporating-system as a part of a spirometer system as described by Kovar et al. was used to supply fresh air and to guarantee a steady air flow.One hour adaptation period was offered to the animals in which no pharmacological treatment occurred. The small glass tube was then filled with 1.5 ml of the respective fragrance material which was constantly released by the slit. A steady concentration by constant drug evaporation from glass tubes throughout the experiment was ensured.TEST ATMOSPHERE- Brief description of analytical method used: The mean air concentration of linaloool in the cage was determined by means of capillary GC and GC/MS as follows: The air stream carrying the fragrance compounds was passed through a layer of activated charcoal which afterwards was eluted with carbon disulfide. After evaporation of the solvent the amount of fragrance compounds remaining in the air was determined by measuring the difference between the original amount of fragrance material in the glass tube and the residual amount in the charcoal. VEHICLENot applicable
Analytical verification of test atmosphere concentrations:
yes
Remarks:
To calculate the air concentration of the fragrance compounds in the cage to consider the total drug volume, charcoal tubes were used.
Duration of exposure:
90 min
Concentrations:
27 mg of linalool in approximately 8.4 litres of cage air -> approximately 3.2 mg/l
No. of animals per sex per dose:
4 animals, no data on sex distribution
Control animals:
yes
Details on study design:
- Duration of observation period following administration: no data- Frequency of observations and weighing: no data- Necropsy of survivors performed: no data- Other examinations performed: the motility of the mice was examined.The mice were kept in a light-barrier cage. The light-barrier, 2 cm above the cagefloor, was interrupted due to the motor activity of the animals crossing it and triggered impulses which were evaluated during the experiment.
Statistics:
Statistics were calculated using an Atari 1040 personal computer ("WISTAT" scientific statistic-package program). The significance was determined by Student's "t"-test and F-test, the level of significance chosen for p to reject the null hypothesis was < 0.05.

Results and discussion

Preliminary study:
Not relevant
Effect levels
Key result
Sex:
male/female
Dose descriptor:
LC50
Effect level:
> 3.2 mg/L air
Exp. duration:
90 min
Remarks on result:
other: no deaths after exposure to 27 mg linalool in 8.4 litres of air
Mortality:
No data
Clinical signs:
other: The exposure led to a decrease in motility of the mice. The observed motility compared to the motility of the untreated control animals was 32%/96% after 30 minutes inhalation, 8%/85% after 60 minutes inhalation and 0%/71% after 90 minutes inhalation for
Body weight:
No data
Gross pathology:
No data
Other findings:
No data

Any other information on results incl. tables

The concentration of linalool in the blood samples following inhalation was approximately 1, 2.8 and 3 ng/ml after 30 minutes, 60 minutes and 90 minutes of exposure, respectively.

Applicant's summary and conclusion

Interpretation of results:
other: causes a decrease in motility in mice
Conclusions:
Linalool caused a decrease in motility in mice in a 90-minute inhalation study.
Executive summary:

The influence of linalool on the motility in mice was tested in an inhalation study under standardized conditions. After 30 minutes, 60 minutes and 90 minutes, the motility in the exposed mice was decreased to 32%/96%, 8%/85% and 0%/71% (6 -8 week old mice/6 month old mice) of the motility of untreated control animals. No deaths were reported at the concentration tested (approximately 3.2 mg/l), therefore the LC50 was determined to be >3.2 mg/l.