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Toxicological information

Skin irritation / corrosion

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Administrative data

Endpoint:
skin irritation: in vivo
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
data from handbook or collection of data
Justification for type of information:
Data is from publication

Data source

Reference
Reference Type:
publication
Title:
The Sensitization Potential of Some Perfume Ingredients Tested Using a Modified Draize Procedure
Author:
D.W. Sharp
Year:
1978
Bibliographic source:
Toxicology, 9 (1978) 261-271

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: Refer below principle
Principles of method if other than guideline:
Preliminary irritation study of Acetophenone (CAS No: 98-86-2) were performed in guinea pigs to determine the concentration for suitable the sensitization study.
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
Acetophenone
EC Number:
202-708-7
EC Name:
Acetophenone
Cas Number:
98-86-2
Molecular formula:
C8H8O
IUPAC Name:
1-phenylethan-1-one
Test material form:
liquid
Details on test material:
Name of the test chemical:1-phenylethanone (Acetophenone)
Molecular Formula:C8H8O
Molecular Weigth:120.15 g/mol
Substance Type: Organic
Physical State: Liquid
Specific details on test material used for the study:
- Name of test material (as cited in study report): Acetophenone
- Molecular formula: C8H8O
- Molecular weight: 120.148 g/mol
- Substance type: Organic
- Physical state: Liquid
-Smiles: c1(ccccc1)C(=O)C
- InChI: 1S/C8H8O/c1-7(9)8-5-3-2-4-6-8/h2-6H,1H3

Test animals

Species:
guinea pig
Strain:
not specified
Details on test animals or test system and environmental conditions:
- Weight at study initiation:450 g

Test system

Type of coverage:
open
Preparation of test site:
other: Intradermal application: clipped and shaved flanks; Dermal application: shaved flanks
Vehicle:
other: Vehicle used but details were not available.
Controls:
not specified
Amount / concentration applied:
Intradermal application: 0.1ml
Dermal application: Amount- 0.1ml
concentration applied -0.25% ICC and 20%ACC
Duration of treatment / exposure:
24 hours
Observation period:
24 hours
Number of animals:
4 guinea pigs(same sex)
Details on study design:
- Area of exposure: small circular areas

Results and discussion

In vivo

Results
Irritation parameter:
other: Intradermal application: erythema and edema Dermal application: erythema
Basis:
mean
Time point:
24 h
Reversibility:
not specified
Remarks on result:
no indication of irritation
Irritant / corrosive response data:
No skin irritating effects were observed.

Applicant's summary and conclusion

Interpretation of results:
other: Not irritating
Conclusions:
No signs of erythema and edema were observed at 0.25% ICC and 20% ACC concentrations. Hence the test material Acetophenone (CAS No: 98-86-2) was considered to be non-irritating on guinea pigs in the preliminary irritation tests performed for the sensitization study.
Executive summary:

Preliminary irritation tests were performed for test chemicalAcetophenone (CAS No: 98-86-2)in guinea pigs to determine the concentration suitableforthe sensitization study[injection challenge concentration (ICC) and application challenge concentration(ACC)].

 

In the preliminary test, the guinea pigs were treated intradermally and dermally.

4 guinea pigs were (same sex) were injected intradermally in the clipped and shaved flanks with 0.1ml aliquots of a range of concentration of test substance in physiological saline. 24 hours later, the skin was examined for erythema and edema.The concentration giving slight but perceptible irritation with no oedema was selected as the injection challenge concentration (ICC).

 

Aliquots of 0.1ml using a range of concentration of the test substance in distilled water were applied in small circular areas to the shaved flanks of 4 previously untreated guinea pigs.24 hours later, the skin was examined for erythema and the highest concentration which caused no irritation was selected as the application challenge concentration (ACC).

 

As a result of preliminary studies, the concentration of Acetophenone at which no irritation was observed were 0.25% for the ICC and 20% for ACC.

 

Since the test chemical did not induce any cutaneous effects at these concentrations, the Acetophenone (CAS No: 98-86-2) was considered to be not irritating to the skin of guinea pigs.