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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: The study was conducted according to the O.E.C.D. test guideline 201 with GLP compliance.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2012
Report date:
2012

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
Deviations:
no
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
4,4'-Isopropylidenediphenol, oligomeric reaction products with 1-chloro-2,3-epoxypropane, reaction products with 2-methylimidazole
EC Number:
500-181-0
EC Name:
4,4'-Isopropylidenediphenol, oligomeric reaction products with 1-chloro-2,3-epoxypropane, reaction products with 2-methylimidazole
Cas Number:
68002-42-6
Molecular formula:
(C15H16O2.C4H6N2.C3H5ClO)x
IUPAC Name:
4,4'-Isopropylidenediphenol, oligomeric reaction products with 1-chloro-2,3-epoxypropane, reaction products with 2-methylimidazole
Test material form:
solid: particulate/powder
Remarks:
migrated information: powder
Details on test material:
As per IUCLID5 Sections 1.1. 1.2. 1.4. and 4.1.

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no
Details on test solutions:
Stock solutions were prepared at concentrations of 25, 50, 100 and 200 mg/L by adding the required nominal amount of test substance in the diluent, algal stock culture medium (Woods Hole) with EDTA up to 0300 mg/L and and mixing the solution for approximately 1 hour.

Test organisms

Test organisms (species):
Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
Details on test organisms:
The green algae Pseudokirchneriella subcapitata was used for the study. The stock cultures were maintained by STILLMEADOW, Inc. cultures in 250-mL sterile Erlenmeyer flasks with stoppers and aeration tubing containing Algal stock culture medium (Woods Hole) with EDTA up to 0.30 mg/L. The test containers received constant aeration and continuous lighting (400 ± 40 ft. c.). The temperature was maintained at 23-25°C.





Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
72 h
Post exposure observation period:
None

Test conditions

Hardness:
No data
Test temperature:
23-25°C
pH:
7.6-7.7 control cultures, 8.6-8.9 test substance treated cultures.
Dissolved oxygen:
Saturated
Salinity:
No data
Nominal and measured concentrations:
6.25, 12.5, 25, 50 and 100 mg/L, nominal.
Details on test conditions:
The test was conducted using concentrations of 6.25, 12.5, 25, 50 and 100 mg/L of the test substance. The test culturesat 50,000 cells/mL
were divided into 250-mL flasks containing the appropriate concentration of test substance (three flasks for each test concentration and six flasks for the control). A positive control group consisted of three flasks treated with 10 mg/L zinc chloride. The flasks were set on a shaker table for constant agitation at approximately 100 cycles/minute. At test initiation, the pH was measured in the control and 100 mgfL concentration. At 24, 48 and 72 hours following dosing, observations of inhibition were made for each test container by measuring the mean cell density with a hemacytometer. The temperature was recorded daily, and the pH of each container was recorded at study termination.
Reference substance (positive control):
yes
Remarks:
Zinc chloride at 10 mg/L.

Results and discussion

Effect concentrations
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
cell number
Details on results:
Based on relative cell number the mean percent survival of the 25 mg/L test substance concentratioin wwas 67% and the relative percent survival at 100 mg/L was 54 % after 72 hr of exposure.
Results with reference substance (positive control):
The mean relative survival of the positive control 10 mg/L zinc chloride cultures was 1.56 % at 24 hr and 0.48 % at 72 hr of exposure.
Reported statistics and error estimates:
he NOEC and EC50 were determined by a commercially available statistical program (ToxCalc Version 5.OTM). The Linear Interpolation model was selected for the determination of the values in this study.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The 72 hr EC50 value based on mean relative cell number was > 100 mg/L of test substances. The mean relative percent survival was approximately 54 % at a test substance concentration of 100 mg/L after 72 hr of exposure.
Executive summary:

The test substance, Bisphenol A, epichlorohydrin polymer, 2-methylimidazole condensate, was accessed for toxicity to green algae in an O.E.C.D. test guideline 201 study. The 72 hr EC50 value based on mean relative cell number was > 100 mg/L of test substances. The mean relative percent survival was approximately 54 % at a test substance concentration of 100 mg/L after 72 hr of exposure.

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