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EC number: 232-235-1
CAS number: 7790-98-9
The results and conclusions of the definitive test are summarized
in the following tables:
> 341 and close to 500
(1) EC100: lowest concentration with
100% immobilization; EC50: Median (50%) Effective Concentration (numbers
in brackets correspond to the 95% confidence limits); EC0: highest
concentration without immobilization
A combined range-finding and limit test (combined range finding
and limit test) was performed to determine the concentrations to be
tested in the definitive test.
Combined range-finding and limit test
In the limit test, two groups of twenty daphnids (in four
replicates of five) were exposed to the test item dissolved in test
water at nominal concentration of 0 and 100 mg/L for 48 hours.
In the range-finding test, four groups of twenty daphnids (in four
replicates of five) were subjected to dilutions of the limit
test solution at 0.1, 1, 10, 200 and 500 mg/L for 48 hours.
In the definitive test, twenty daphnids (in four replicates of
five) were exposed to the nominal concentrations of 0, 108, 158, 232,
341 and 500 mg/L for 48 hours.
As the tests were performed in static conditions the solutions
remained unchanged for the duration of the test.
Immobilization at each concentration was recorded at 0, 24 and
Samples weretaken to measure the concentration of the test item in
each test solution of the definitive test, except for the control.
All validity criteria of the study were respected for each test.
Measured concentrations in the definitive test solutionswere
within ± 20% of the corresponding nominal values throughout the test
(Table A, Appendix 3). The test item was therefore stable under our
experimental conditions and the study results were based on nominal
No difficulty was experienced in discerning the daphnids during
the observation periods.
Apart from immobilization, no other adverse effects were observed
in the control and treated daphnids.
The 24 and 48-hour EC50s were not calculated since immobilization
at the highest concentration (500 mg/L) was lower than 50% at the
corresponding time (20, 40%, respectively).
However in the combined range finding and limit test it was
observed an immobilization of 60% at T48 hours, therefore it was assumed
that the 48-hour EC50 is close to 500 mg/L.
In a 48-hourstaticacute toxicity test with Daphnia magna,
the EC50 of Perchlorate d’ammonium was > 341 mg/L and estimated to be
close to 500 mg/L according to the combined results of the combined
range-finding and limit test and the definitive test.
The No Observed Effect Concentration NOEC was ≥ 232 mg/L.
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