Reaction products of 2-(chloromethyl)oxirane and glycerol

Endpoint summary

Description of key information

Short term toxicity to fish

The LC50(96h) value of test item was calculated to be 772 mg/L using the Danish QSAR battery model which integrate predictions from two individual models for the same endpoint. This prediction falls in the applicability domain of the model.

Long term toxicity to fish

The chronic toxicity to fish of the test substance was predicted by the computer program ECOSAR v1.00 (EPIWIN software) by US-EPA (Chemservice S.A., 2015c).

The computer program ECOSAR v1.00 detected “epoxides, poly” among others as ECOSAR-Class to predict the aquatic chronic toxicity for 1,2,3-Propanetriol, Glycidyl ethers. This class is considered as most suitable class for the substance. A Chronic Values (ChV; 30 d) of 9.8 mg/L for fish was predicted for 1,2,3-Propanetriol, Glycidyl ethers with chlorine included in the main component. Without chlorine, the value differs only to a minimal extend, a ChV of 10.2 mg/L for fish is determined.

Short term toxicity to aquatic invertebrates

The short term toxicity to aquatic invertebrates of test item was determined to be EC50(48h) 105 mg/L using the Danish QSAR battery model which integrate predictions from two individual models for the same endpoint. The prediction was in the applicability domain of the model.

Long term toxicity to aquatic invertebrates

Aquatic invertebrate reproduction test_Daphnia magna_OECD 211/EU Method C.20: NOEC(21d): 8.3 mg/L, LOEC(21d): 26 mg/L (both: mean measured concentrations), EC50(21d) > 100 mg/L (nominal)

Toxicity to aquatic algae and cyanobacteria

Algal growth inhibition test_Pseudokirchneriella subcapitata_OECD 201/EU Method C.3: ErC10(72h), ErC20(72h) and ErC50(72h) > 120 mg/L / EbC10(72h): 7.7 mg/L, EbC20(72h): 120 mg/L (extrapolated) and EbC50(72h) > 120 mg/L (extrapolated). NOEC(72h): 21 mg/L. NOEC(72h): 69 mg/L
Calculation with ECOSAR v0.99g and v1.00 (EPIWIN software by US-EPA):
Main component with chlorine: EC50(96h): 14324 mg/L; ChV(4d): 11676.9 mg/L
Main component without chlorine: EC50(96h): 33823 mg/L; ChV(4d): 12795.8 mg/L

Toxicity to microorganisms

Activated sewage sludge, OECD 209: EC50(3h) > 1000 mg/L, NOEC(3h): 1000 mg/L

Additional information

The toxicity of 1,2,3-Propanetriol, glycidyl ethers towards the aquatic biota was not investigated experimentally for all trophic levels. Guideline studies with GLP compliance were conducted regarding long-term toxicity to aquatic invertebrates (Liedtke, 2013), toxicity to algae (Liedtke, 2013) and to aquatic microorganisms (Wadsley, 2012). Other endpoints and supporting information are fulfilled using the scientifically acceptable QSAR software Danish QSAR Database.

Liedtke (2013) investigated the long-term toxicity of the test substance towards Daphnia magna in accordance with OECD Guideline 211 / EU Method C.20. The semi-static test was conducted for 21 days with test medium renewals every 48 or 72 hours. The nominal test concentrations were chosen as: 1.0, 3.2, 10, 32 and 100 mg/L with 10 replicates per concentration. Based on the mentioned observations, it can be stated, with the exception of the reduced reproduction rates, no visible abnormalities were observed at the test animals during the experiment. The final results, based on survival and reproduction of the test animals can be reported as followed: NOEC(21d): 8.3 mg/L and LOEC(21d): 26 mg/L as mean measured concentrations. The EC50 value was clearly higher than the highest tested concentration of nominal 100 mg/L, because none of the responses exceeded 50 %.

Pseudokirchneriella subcapitata was used as test organism in an experiment conducted according to OECD Guideline 201 / EU Method C.3 (Liedtke, 2013). Nominal test concentrations of 0.32, 1.0, 3.2, 10, 32 and 100 mg/L were chosen for the definitive test. The biological results based on growth rate can be summarized as follows: ErC10(72h), ErC20(72h) as well as ErC50(72h) were found to be > 120 mg/L. Based on yield, the results were reported as: EbC10(72h): 7.7 mg/L, EbC20(72h): 120 mg/L (extrapolated) and EbC50(72h) > 120 mg/L (extrapolated). The NOEC was found as 21 mg/L and the LOEC as 69 mg/L after 72 hours exposure duration, respectively.

Concerning toxicity to aquatic microorganisms, an experiment was conducted in accordance to OECD Guideline 209 with activated sewage sludge, obtained from a predominantly domestic sewage (Wadsley, 2012). The following test concentrations were employed: test concentrations of 10, 100 and 1000 mg/L. Up to the highest concentration tested, no negative effects on the inoculum were observed. Therefore, the EC50(3h) is considered to be > 1000 mg/L, the corresponding NOEC is 1000 mg/L.