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EC number: 701-177-3 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 08 Mar 1989
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Version / remarks:
- adopted in 1984
- GLP compliance:
- no
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test item was directly added to the test medium. - Test organisms (species):
- activated sludge
- Details on inoculum:
- - Source of inoculum: Activated sludge was collected from the sewage treatment plant of Therwil, Switzerland on 06 Mar 1989.
- Preparation of inoculum for exposure: The sludge was separated from the aqueous layer by settling. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Test temperature:
- 18 - 20 °C
- Nominal and measured concentrations:
- Nominal: 0, 1.1, 3.3, 10.7, 32.0, 100.2 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: BOD flasks with gas inlet
- Size, fill volume: 250 mL, 200 mL
- Aeration: Yes
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 2
- Concentration of sludge: 1.6 g/L
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Dechlorinated drinking water
EFFECT PARAMETERS MEASURED (with observation intervals if applicable)
- Microbial respiration was measured after 3 h of incubation.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: approx. 3.2 - Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol
- Duration:
- 3 h
- Dose descriptor:
- other: IC20
- Effect conc.:
- 75 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 3 h
- Dose descriptor:
- IC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- - Validity criteria are fulfilled.
- Results with reference substance (positive control):
- - Results with reference substance valid? Yes
- Relevant effect levels: IC50 = 13 mg/L - Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 09 Sep 2009
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Version / remarks:
- adopted in 1984
- Deviations:
- yes
- Remarks:
- Higher temperature during the test (Guideline: 18 - 20 °C, Test: 23.3 - 24.4 °C)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
- Version / remarks:
- adopted in 2008
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Landesamt für Umwelt, Wasserwirtschaft und Gewerbeaufsicht, Mainz, Germany
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A stock solution containing 10 g/L was prepared using the following procedure: 10 g test item was mixed with 1.1 g NaOH (45%). 40 mL deionised water (80 – 100 °C) was added. After homogenisation with a glass stirrer, 85 mL deionised water (room temperature) was added. Then, the vessel was stirred on a magnetic stirrer. 0.5 M NaOH was added until the resulting solution was clear. The pH of the solution was adjusted to 7.8 ± 0.2 with 1 M HCl. the solution was transferred into a volumetric flask and filled up to 1000 mL with deionised water. - Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Source of the inoculum: The sludge was taken from the activation basin of the sewage plant in Edenkoben, Germany
- Laboratory culture: Upon arrival, the sludge was washed with tap water three times, centrifuged and resuspended in tap water.
- Suspended solids: 4.2 g/L - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- pH:
- 7.7 - 8.1
- Nominal and measured concentrations:
- Nominal: 0, 1.0, 3.2, 10, 32, 100, 320, 1000 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Beakers
- Material, fill volume: Glass, 500 mL
- Aeration: Purified air at 0.75 L/min
- No. of vessels per concentration (replicates): 2 for treatment 1000 mg/L, 1 for all other treatments and positive control
- Concentration of suspended solids: 1.7 g/L
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: tap water
- Total organic carbon: < 1.0 mg/L
- Metals (mg/L): Na 4.7, K 2.6, Ca 33.1, Mg 2.1, other metals < 0.0005 to < 0.01
- Pesticides: < LOD
- Chloride: 9.0 mg/L
- Conductivity: 189 µS/cm
- Intervals of water quality measurement: pH was measured at test end.
EFFECT PARAMETERS MEASURED (with observation intervals if applicable)
- Respiration rate was measured after 3 h of incubation
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 3.2 - Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol
- Duration:
- 3 h
- Dose descriptor:
- other: EC20
- Effect conc.:
- 130 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: 95% CL = 62 - 310 mg/L
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 1 300 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: 95% CL = 480 - 7200 mg/L; extrapolated
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 10 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- - The inhibition caused by the test item did not rise above 45%. Therefore the EC50(3h) was determined by extrapolation.
- All validity criteria are fulfilled. - Results with reference substance (positive control):
- - Results with reference substance valid? Yes
- EC50: 9.1 mg/L, 95% CL = 7.2 - 11.0 mg/L
Referenceopen allclose all
Table 1: Inhibition of microbial respiration
Concentration (mg/L) |
Consumption Rate (mg/L*h) |
Inhibition (%) |
Blank 1 |
40.62 |
2 |
Blank 2 |
42.21 |
-2 |
1.1 |
41.62 |
0 |
3.3 |
43.97 |
-6 |
10.7 |
41.79 |
-1 |
32.0 |
35.70 |
14 |
100.2 |
30.00 |
28 |
Table 1: Inhibition (%) of microbial respiration after 3 h of incubation
Concentration |
% Inhibition |
Control |
- |
1.0 |
-3.2 |
3.2 |
1.2 |
10 |
1.7 |
32 |
16.9 |
100 |
25.5 |
320 |
24.0 |
1000 (1) |
43.8 |
1000 (2) |
45.3 |
Description of key information
NOEC (3h) = 10 mg/L (OECD 209)
Key value for chemical safety assessment
Additional information
Two studies investigating the toxicity to microorganism of N-methyl-N-(C18-(unsaturated)alkanoyl)glycine (EC 701-177-3) are available.
The key study was carried out according to OECD 209 under GLP-conditions with domestic activated sludge as inoculum. In this static test seven test concentrations were used (1.0, 3.2, 10, 32, 100, 320 and 1000 mg/L). The dry matter was determined as 4.2 g suspended solids/L, giving a concentration of 1.7 g suspended solids/L in the test. The highest inhibition of respiration rate was determined for the highest tested concentration (1000 mg/L nominal) with 45.3% and 43.8% inhibition. Thus, the inhibition of respiration rate is < 50%. In the study report the EC50 was extrapolated with an EC50 (3h) of 1300 mg/L. But as an extrapolation is imprecise, the EC50 (3h) > 1000 mg/L (nominal) could be stated. The NOEC (3h) was reported as 10 mg/L.
The supporting study was conducted according to OECD 209 under non-GLP conditions. Five test concentrations (1.1, 3.3, 10.7, 32.0, 100.2 mg/L) were inoculated to activated sludge. In this study, the IC50 (3h) for respiration inhibition was above the highest test concentration of 100 mg/L. A NOEC of 10 mg/L is reported.
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