Perfluorooctane

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro DNA damage and/or repair study

Remarks:

Type of genotoxicity: DNA damage and/or repair

Type of information:

read-across based on grouping of substances (category approach)

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Justification for type of information:

Saturated perfluorocarbons have very similar toxicological properties and so a "read across" approach is justified.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1979

Materials and methods

Test guideline

Qualifier:

no guideline available

Principles of method if other than guideline:

Salmonella/mammalian-microsme test, performed twice in presence and absence of rat liver homogenate metabolic activation system. Tested at various concentrations.

GLP compliance:

no

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

Perflunafene

EC Number:

206-192-4

EC Name:

Perflunafene

Cas Number:

306-94-5

Molecular formula:

C10F18

IUPAC Name:

1,1,2,2,3,3,4,4,4a,5,5,6,6,7,7,8,8,8a-Octadecafluoronaphthalene

Test material form:

other: vapour

Method

Target gene:

Histidine operon

Species / strain

Species / strain / cell type:

S. typhimurium TA 1535, TA 1537, TA 98 and TA 100

Additional strain / cell type characteristics:

other: defective lipopolysaccharide coat, deleted vitamin biotin synthesis gene, deleted UV damage repair gene

Metabolic activation:

with and without

Metabolic activation system:

rat liver homogenate

Test concentrations with justification for top dose:

2, 1.6, 1.2, 0.8, 0.4, 0.1
ml of liquid vaporised in 9 l desicator (the highest concentration is approximately the vapour pressure at 37°C)

Results and discussion

Test results

Species / strain:

S. typhimurium TA 1535, TA 1537, TA 98, TA 100 and E. coli WP2

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

no cytotoxicity

Vehicle controls validity:

not specified

Untreated negative controls validity:

valid

Positive controls validity:

valid

Additional information on results:

""None of the eight taggant samples tested appeared to be mutagenic in any of these assays"

Remarks on result:

other: all strains/cell types tested

Remarks:

Migrated from field 'Test system'.

Applicant's summary and conclusion

Conclusions:

Interpretation of results (migrated information):
negative

Material dos not cause genetic toxocity.

Executive summary:

Testing concluded the material had no ability to induce mutations under test conditions.