Diallyl 2,2'-oxydiethyl dicarbonate

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1982-10-26 to 1982-10-28

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

test procedure in accordance with national standard methods with acceptable restrictions

Qualifier:

equivalent or similar to guideline

Guideline:

EPA OPP 72-2 (Aquatic Invertebrate Acute Toxicity Test)

Deviations:

not specified

Principles of method if other than guideline:

test followed the "Methods for acute toxicity tests with fish, macroinvertebrates, and amphibians" (US-EPA-660/3-75-009; 1975),

GLP compliance:

no

Analytical monitoring:

no

Remarks:

But due to good water solubility and slow hydrolysis the test item is expected to be stable for the duration of the test.

Details on sampling:

- Concentrations: control, 6.4, 11, 18, 30 and 50 mg/L (nominal concentrations)
- Sampling method: No analytical verification of nominal test substance concentrations

Vehicle:

no

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna
- Source: The water fleas used in this toxicity test were obtained from laboratory stocks cultured at EG&G, Bionomics.
- Age at study initiation (mean and range, SD): <= 24 hours old
- Method of breeding: no information
- Feeding during test
- Food type: no feeding

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Post exposure observation period:

none

Hardness:

Total hardness and alkalinity ranges as calcium carbonate (CaC03) of 150-180 mg/L and 100-130 mg/L, respectively.

Test temperature:

The temperature in the holding tank was 22-21°C:
At 0 hours: 21°C,
At 48 h: 22°C.

pH:

At 0 hours: 8.3,
At 48 h: 8.2 to 8.4.

Dissolved oxygen:

DO (mg/l):
At 0 hours: 8.9,
At 48 h: 8.6 to 8.7

Salinity:

specific conductance of 400-600 micromhos

Nominal and measured concentrations:

6.4, 11, 18, 30 and 50 mg/L (nominal concentrations);
no analytical verification of nominal test substance concentrations

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: Glass beakers, Size 250 mL, filled with 150 mL test solution
- Aeration: None
- Type of flow-through (e.g. peristaltic or proportional diluter): Static test conditions
- Renewal rate of test solution (frequency/flow rate): None
- No. of organisms per vessel: Fifteen water fleas (5 per beaker)
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: The culture water was prepared by fortifying well water according to the formula for hard water presented by U.S. EPA (1975) and filtering it through an Amberlite XAD-7 resin column to remove any potential organic contaminants.
- Alkalinity: Water had a total hardness and alkalinity as calcium carbonate (CaC03) of 150-180 mg/L and 100-130 mg/L, respectively,
- Conductivity: specific conductance of 400-600 micromhos
- Culture medium different from test medium:
- Intervals of water quality measurement: The pH's and DO concentrations were measured at 0 and 48 hours in one replicate of the control and the high, middle and low test concentrations. The temperature was measured at 0 and 48 hours in one replicate of the control.


OTHER TEST CONDITIONS
- Adjustment of pH: 7.9 to 8.3
- Photoperiod: Illumination during test duration
- Light intensity: The test area was illuminated with Durotest (Optima) fluorescent lights at an intensity of 430-760 hlx.


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Mortalities in replicate test solutions were recorded at 24 and 48 hours exposure. Biological observations and observations of the physical characteristics of each replicate test solution were also made and recorded at 0, 24 and 48 hours.

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

18 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other: estimated by binomial probability; 95% CL = 11-30

Duration:

24 h

Dose descriptor:

LC50

Effect conc.:

23 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other: estimated by binomial probability; 95% CL = 18-30

Duration:

48 h

Dose descriptor:

LC0

Effect conc.:

11 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: mortality, mobility

Details on results:

The concentrations tested and the corresponding mortality data derived from the toxicity test were used to estimate 24- and 48-hour median lethal concentrations (LC50) and 95% confidence intervals. The 48-hour LC50 (and 95% confidence interval) for water flea to CR-39, estimated by binomial probability, was 18 (11-30) mg/L .

Results with reference substance (positive control):

not relevant

Reported statistics and error estimates:

The concentrations tested and the corresponding mortality data derived from the toxicity test were used to estimate 24- and 48-hour median lethal concentrations (LC50) and 95% confidence intervals. The computer program utilized (Stephan, 1978, personal communication) estimated LC50 values using one of three statistical methods in the following order of preference: moving average angle analysis, prohit analysis, binomial probability. The method selected was determined by the characteristics of the data base (i.e. presence or absence of concentrations causing mortality of 100% of the animals in the test population, number of concentrations causing mortality of a partial number of animals in the test population, etc.). The computer program scanned the data base, identified the most appropriate statistical method and performed the analysis.

Table: Concentrations tested and corresponding observed percentage mortalities for the water flea (Daphnia magna) exposed to CR-39:

Nominal concentration (mg/L)	Percentage mortality
	24 h				48 h
	A	B	C	X	A	B	C	X
50	100	100	100	100	100	100	100	100
30	100	100	100	100	100	100	100	100
18	0	0	0	0	20	0	80	33
11	0	0	0	0	0	0	0	0
6.4	0	0	0	0	0	0	0	0
control	0	0	0	0	0	0	0	0

Validity criteria fulfilled:

yes

Conclusions:

The 48-hour LC50 and 95% confidence interval for the water flea exposed to CR-39 were estimated by binomial probability to be 18 (11-30) mg/L.

Executive summary:

The purpose of this study was to estimate the acute toxicity of CR-39 to the water flea (Daphnia magna) under static conditions. Test followed the "Methods for acute toxicity tests with fish, macroinvertebrates, and amphibians" (US-EPA-660/3-75-009; 1975).

The 48-hour LC50 and 95% confidence interval for the water flea exposed to CR-39 were estimated by binomial probability to be 18 (11-30) mg/L.

Reference 2

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

1982-10-25 to 1982-10-29

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

test procedure in accordance with national standard methods with acceptable restrictions

Qualifier:

no guideline available

Principles of method if other than guideline:

test followed internal procedures of the laboratory (Static acute Mysid shrimps toxicity test, June 1982)

GLP compliance:

yes

Analytical monitoring:

no

Details on sampling:

- Concentrations: control, 0, 6.25, 12.5, 25, 50 and 100 mg/L (nominal concentrations)
- Sampling method: No analytical verification of nominal test substance concentrations

Vehicle:

no

Test organisms (species):

Americamysis bahia (previous name: Mysidopsis bahia)

Test type:

static

Water media type:

saltwater

Limit test:

no

Total exposure duration:

96 h

Post exposure observation period:

None

Nominal and measured concentrations:

0, 6.25, 12.5, 25, 50 and 100 mg/L (nominal concentrations);
no analytical verification of nominal test substance concentrations

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

70.7 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other: 95% CL = 55.6-77.6

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

12.5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Duration:

72 h

Dose descriptor:

LC50

Effect conc.:

84.7 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other: 95% CL = 81.4-88.2

Duration:

72 h

Dose descriptor:

LC0

Effect conc.:

12.5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Duration:

48 h

Dose descriptor:

LC0

Effect conc.:

12.5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Duration:

24 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Duration:

24 h

Dose descriptor:

LC0

Effect conc.:

12.5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Details on results:

.

After 24 hours of exposure to CR-39, mortality of mysid shrimp ranged from 0% in the 6.25, 12.5, and 100 ppm test concentrations to 10% in the 50 ppm test concentration. The 24-hour LC50 was >100 ppm. After 96 hours of exposure, mortality ranged from 0% in the 6.25 and 12.5 ppm test concentrations to 70% in the 100 ppm test concentration. There was no mortality in the seawater control. Mortality was 5% in the solvent control. The 96-hour LC50 was 70.7 ppm with 95% confidence limits of 55.6-77.6 ppm.

Table: Mortality of mysid shrimp exposed to CR-39 in static, unaerated seawater

Nominal concentration (mg/L; ppm)	Cumulative mortality [%]
	24 h	48 h	72 h	96 h
Seawater control	0	0	0	0
Solvent control	5	5	5	5
6.25	0	0	0	0
12.5	0	0	0	0
25.0	5	15	15	15
50.0	10	20	20	30
100.0	0	40	60	70

Validity criteria fulfilled:

yes

Conclusions:

The 96-hour LC50 was 70.7 ppm with 95% confidence limits of 55.6-77.6 ppm

Executive summary:

A static marine toxicity test was conducted at EG&G Bionomics Marine Research Laboratory (BMRL), Pensacola, Florida, to determine the acute effect of CR-39 on mysid shrimp (Mysidopsis bahia). The criterion for effect was death. Results of the test are expressed as 24-, 48-, 72-, and 96-hour LC50's (the concentrations of the test material estimated to be lethal to 50% of the test animals at the specified times). The 96-hour LC50 was 70.7 ppm with 95% confidence limits of 55.6-77.6 ppm.

Description of key information

- LC50 (48h) = 18 mg/L for Daphnia magna (guideline equivalent or similar to EPA OPP 72 -2 (Aquatic Invertebrate Acute Toxicity Test); static)
- LC50 (96h) = 70.7 mg/L for Mysidopsis bahia (test followed internal procedures of the laboratory (Static acute Mysid shrimps toxicity test, June 1982))

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Dose descriptor:

LC50

Effect concentration:

18 mg/L

Marine water invertebrates

Marine water invertebrates

Dose descriptor:

LC50

Effect concentration:

70.7 mg/L

Additional information

This endpoint is filled from data from one study in Daphnia magna (Suprenant et al., 1982) and another in Mysidopsis bahia (mysid shrimp) (Scott Ward, 1982a). The 48-hour EC50 value for 'diallyl 2,2'-oxydiethyl dicarbonate' in Daphnia magna is 18 mg/L and the 96-hour EC50 value in Mysidopsis bahia is 70.7 mg/L.