Registration Dossier

Diss Factsheets

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
15 - 17 Mar 1993
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1993
Report date:
1993

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
Qualifier:
according to guideline
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
Deviations:
no
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
1,4:3,6-dianhydro-2,5-di-O-methyl-D-glucitol
EC Number:
226-159-8
EC Name:
1,4:3,6-dianhydro-2,5-di-O-methyl-D-glucitol
Cas Number:
5306-85-4
Molecular formula:
C8H14O4
IUPAC Name:
1,4:3,6-dianhydro-2,5-di-O-methyl-D-glucitol

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: A 1000 mg/L stock solution of the test substance was prepared by dissolving 1 g of test substance in 1 L of dilution water.
- Eluate: no
- Differential loading: no
- Controls: yes, test medium control

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: water flea (Daphnia magna Straus)
- Source: in-house culture
- Age at study initiation: less then 24 h
- Method of breeding: culture maintained in reconstituted water medium identical to test dilution water at temperature 20 ± 2 °C. The cultures were fed using algal diet (chlorella vulgaris) and microencapsulated feed Frippak Booster TM. Reproduction by diploid parthenogenesis.
- Feeding during test: none

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Post exposure observation period:
none

Test conditions

Hardness:
173 mg/L (as CaCO3)
Test temperature:
20.2 - 20.4 °C
pH:
8.24 - 8.43
Dissolved oxygen:
8.7 - 9.4 mg O2/L
Nominal and measured concentrations:
0 and 1000 mg/L (nominal concentration)
Details on test conditions:
TEST SYSTEM
- Test vessel: 250 mL glass beakers with loose fitting lids filled with 200 mL test solution
- Aeration: water was aerated for 2 hours before use. No aeration during test phase.
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4


TEST MEDIUM / WATER PARAMETERS
reconstituted water

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 h light/ 8 h dark (with a 15 min transition period)
- Light intensity: not stated

EFFECT PARAMETERS MEASURED: mobility after 24 and 48 hours
Reference substance (positive control):
no

Results and discussion

Effect concentrations
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
- Behavioural abnormalities: none
- Mortality of control: none
- Abnormal responses: none
Results with reference substance (positive control):
not available

Any other information on results incl. tables

Immobilization of D. magna after 24 and 48 hours:

 Test substance concentration (mg/L) Immobilization [%]
24h 48h
0 0 0
1000 0 0

Applicant's summary and conclusion

Categories Display