1,4:3,6-dianhydro-2,5-di-O-methyl-D-glucitol

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

15 - 17 Mar 1993

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1993

Report date:

1993

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

1,4:3,6-dianhydro-2,5-di-O-methyl-D-glucitol

EC Number:

226-159-8

EC Name:

1,4:3,6-dianhydro-2,5-di-O-methyl-D-glucitol

Cas Number:

5306-85-4

Molecular formula:

C8H14O4

IUPAC Name:

1,4:3,6-dianhydro-2,5-di-O-methyl-D-glucitol

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: A 1000 mg/L stock solution of the test substance was prepared by dissolving 1 g of test substance in 1 L of dilution water.
- Eluate: no
- Differential loading: no
- Controls: yes, test medium control

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: water flea (Daphnia magna Straus)
- Source: in-house culture
- Age at study initiation: less then 24 h
- Method of breeding: culture maintained in reconstituted water medium identical to test dilution water at temperature 20 ± 2 °C. The cultures were fed using algal diet (chlorella vulgaris) and microencapsulated feed Frippak Booster TM. Reproduction by diploid parthenogenesis.
- Feeding during test: none

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Post exposure observation period:

none

Test conditions

Hardness:

173 mg/L (as CaCO3)

Test temperature:

20.2 - 20.4 °C

pH:

8.24 - 8.43

Dissolved oxygen:

8.7 - 9.4 mg O2/L

Nominal and measured concentrations:

0 and 1000 mg/L (nominal concentration)

Details on test conditions:

TEST SYSTEM
- Test vessel: 250 mL glass beakers with loose fitting lids filled with 200 mL test solution
- Aeration: water was aerated for 2 hours before use. No aeration during test phase.
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4


TEST MEDIUM / WATER PARAMETERS
reconstituted water

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 h light/ 8 h dark (with a 15 min transition period)
- Light intensity: not stated

EFFECT PARAMETERS MEASURED: mobility after 24 and 48 hours

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

- Behavioural abnormalities: none
- Mortality of control: none
- Abnormal responses: none

Results with reference substance (positive control):

not available

Any other information on results incl. tables

Immobilization of D. magna after 24 and 48 hours:

Test substance concentration (mg/L)	Immobilization [%]
	24h	48h
0	0	0
1000	0	0