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EC number: 226-159-8 | CAS number: 5306-85-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 05 Dec 2016 - 11 Apr 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 017
- Report date:
- 2017
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Medicines & Healthcare products Regulatory Agency, UK
Test material
- Reference substance name:
- 1,4:3,6-dianhydro-2,5-di-O-methyl-D-glucitol
- EC Number:
- 226-159-8
- EC Name:
- 1,4:3,6-dianhydro-2,5-di-O-methyl-D-glucitol
- Cas Number:
- 5306-85-4
- Molecular formula:
- C8H14O4
- IUPAC Name:
- 1,4:3,6-dianhydro-2,5-di-O-methyl-D-glucitol
- Test material form:
- liquid
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: control and 100 mg/L
- Sampling method: duplicate samples were taken at 0 and 72 hours of the test.
- Sample storage conditions before analysis: The samples were stored frozen prior to analysis.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: A 100 mg/L stock solution of the test substance was prepared by dissolving 100 mg of test substance in 1 L of dilution water.
- Eluate: no
- Differential loading: no
- Controls: yes, test medium control
Test organisms
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Common name: green algae
- Strain: CCAP 278/4
- Source (laboratory, culture collection): Culture Collection of Algae and Protozoa (CCAP), SAMS Research Services Ltd, Scottish Marine Institute, Oban, Argyll, Scotland.
- Method of cultivation: Master cultures were maintained in the laboratory by the periodic replenishment of culture medium. The master cultures were maintained under constant aeration and illumination at 21 ± 2 °C.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 72 h
- Post exposure observation period:
- none
Test conditions
- Test temperature:
- 24 ± 1 ºC
- pH:
- Control:
7.6 at 0 hours
8.4 at 72 hours
Test concentration 100 mg/L:
7.7 at 0 hours
8.5 at 72 hours - Nominal and measured concentrations:
- 0 and 100 mg/L (nominal concentration)
0 and 98.7 mg/L (measured concentrations at T = 0 h); 0 and 97.8 mg/L (at T = 72 h) - Details on test conditions:
- TEST SYSTEM
- Test vessel: 250 mL glass conical flasks
- Type (delete if not applicable): closed with polyurethane foam caps
- Material, size, headspace, fill volume: 250 mL
- Aeration: no, but constantly shaken at 150 rpm
- Initial cells density: 5 x 10^3 cells per mL
- Control end cells density: 8.2 x 10^5 cells per mL (mean)
- No. of vessels per concentration (replicates): 6
- No. of vessels per control (replicates): 6
GROWTH MEDIUM
- Standard medium used: yes
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Reverse osmosis purified deionized water and the pH adjusted to 7.5 with NaOH or HCl.
OTHER TEST CONDITIONS
- Sterile test conditions: no
- Adjustment of pH: yes
- Photoperiod: continuous illumination
- Light intensity and quality: ca. 7000 lux; wave 380 – 730 nm
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: Cell density was measured at 24, 48 and 72 hour using Coulter® Multisizer Particle Counter.
TEST CONCENTRATIONS
- Range finding study
- Test concentrations: 0, 0.10, 1.0, 10, 100 mg/L
- Results used to determine the conditions for the definitive study: Maximum test concentration (100 mg/L) had no effect on algae growth. - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Details on results:
- Growth rate and yield of green algae P. subcapitata were not affected by the test item at a nominal test concentration of 100 mg/L over the 72-hour exposure period.
- Results with reference substance (positive control):
- 72 h ErC50 = 1.4 mg/L; NOECr = 0.25 mg/L
72 h EyC50 = 0.6 mg/L; NOECy = 0.25 mg/L - Reported statistics and error estimates:
- Student’s t-test incorporating Bartlett's test for homogeneity of variance (Sokal and Rohlf, 1981).
Any other information on results incl. tables
Cell Densities and Percentage Inhibition of Growth from the Range-finding Test:
Nominal Concentration (mg/L) |
Mean Cell Densities (cells per mL) | Inhibition Values (%) | ||
0 h | 72 h | Growth rate | Yield | |
control | 9.06E+03 | 1.46E+06 | - | - |
0.1 | 9.03E+03 | 1.25E+06 | 4 | 14 |
1 | 8.61E+03 | 1.34E+06 | 1 | 8 |
10 | 9.22E+03 | 1.26E+06 | 4 | 14 |
100 | 9.40E+03 | 1.27E+06 | 4 | 13 |
Applicant's summary and conclusion
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