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Environmental fate & pathways

Biodegradation in water and sediment: simulation tests

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Administrative data

Endpoint:
biodegradation in water and sediment: simulation tests
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Study based on accepted scientific principles, limited documentation of the test method, acceptable for assessment

Data source

Reference
Reference Type:
publication
Title:
Microbial and photochemical degradation of polycyclic aromatic hydrocarbons in estuarine waters and sediments
Author:
Lee RF, Ryan C
Year:
1983
Bibliographic source:
Can. J. Fish. Aquat. Sci., 40(Suppl. 2), 86-94 (1983)

Materials and methods

Principles of method if other than guideline:
Biodegradation test in water and sediment slurry under shaking, using 14C-radiolabelled test substances.
Test conditions of Lee and Ryan (1983) did not resemble conditions required at the present for simulation tests.
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): anthracene
- Specific activity (if radiolabelling): 76.6 MBq/mmol
- Locations of the label (if radiolabelling): 9-[14C]
Radiolabelling:
yes

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
other: water and sediment, adapted to different degree, marine
Details on source and properties of surface water:
Water and sediment were collected from one estuarine in Charleston, SC, contaminated with oil, and from a “cleaner” estuarine of Savannah, GA.
Further sediment samples were taken from MERL mesocosm tanks of Narragansett Bay, RI.
Duration of test (contact time):
>= 3 d
Initial test substance concentrationopen allclose all
Initial conc.:
1 other: mg/kg sediment
Based on:
test mat.
Initial conc.:
2.5 other: mg/kg sediment
Based on:
test mat.
Initial conc.:
5 other: mg/kg sediment
Based on:
test mat.
Parameter followed for biodegradation estimation:
CO2 evolution
Details on study design:
1. Test with sediment, sediment-seawater slurry (Charleston and Savannah), consisting of 1 g of sediment and 50 ml of seawater in 125 mL bottles.
Test concentration of 14C-labelled anthracene: 2.5 mg/kg (no information whether dwt or wwt).
Temperature: 27 and 28 °C .
The flasks were carried out as triplicates.
Abiotic sterile control: with mercuric chloride added (10mg/g sediment)


2. Test with sediment, sediment-seawater slurry, sediments taken from MERL mesocosm tanks (Narragansett Bay, Rhode Island)
Temperature: 18 °C

Results and discussion

Mean total recovery
Compartment:
other: water / sediment, material (mass) balance
Remarks on result:
other: no data
Half-life of parent compound / 50% disappearance time (DT50)open allclose all
Compartment:
entire system
DT50:
210 d
Type:
not specified
Remarks on result:
other: Savannah: "cleaner" area, 2.5 mg/kg, 27 °C
Compartment:
entire system
DT50:
57 d
Type:
not specified
Remarks on result:
other: Charleston: contaminated area, 2.5 mg/kg, 28 °C
Compartment:
entire system
DT50:
>= 79 - <= 99 d
Type:
not specified
Remarks on result:
other: Narragansett Bay: 1, 2.5 and 5 mg/kg in the control mesocosm at 18 °C
Compartment:
entire system
DT50:
>= 5 - <= 7 d
Type:
zero order
Remarks on result:
other: Narragansett Bay: 1, 2.5 and 5 mg/kg in the oil-pre-treated mesocosm at 18 °C

Any other information on results incl. tables

no further data

Applicant's summary and conclusion