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Environmental fate & pathways

Bioaccumulation: aquatic / sediment

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Administrative data

Endpoint:
bioaccumulation in aquatic species: fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1977
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Comparable to guideline study

Data source

Referenceopen allclose all

Reference Type:
other: data bank
Title:
Anthracene - Biodegradation and Bioconcentration of Existing Chemical Substances under teh Chemical Substances control Law
Author:
NITE
Year:
2002
Bibliographic source:
www.safe.nite.go.jp/data/hazkizon/pk_e_kizon_input_second.home_object
Report Date:
1977
Reference Type:
other: data bank
Title:
Unnamed
Report Date:
1992
Reference Type:
secondary source
Title:
REQUEST for information on BCF testing (Inquiry into Japanese Studies of Bioconcentration in Fish)
Author:
de Bruijn J
Year:
2003
Bibliographic source:
E.mail message to EU Member States and CEFIC
Report Date:
2003

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to
Guideline:
OECD Guideline 305 (Bioconcentration: Flow-through Fish Test)
Version / remarks:
May 12, 1981
Deviations:
yes
Remarks:
Elimination (depuration) not determined
Qualifier:
according to
Guideline:
other: Japan Ministry of Health and Welfare as well as MITI No. 1 , 1974
Principles of method if other than guideline:
in accordance with Japanese Industrial Standard (JIS K 0102-1986-71), titled "Testing methods for industrial waste water"
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): anthracene
- Molecular formula (if other than submission substance): C14H10
- Molecular weight (if other than submission substance):
- Substance type: organic
- Physical state: solid
- Analytical purity: >= 96%
- Impurities (identity and concentrations): no data
Radiolabelling:
no

Test solutions

Vehicle:
yes
Details on preparation of test solutions, spiked fish food or sediment:
PREPARATION OF SPIKED WATER
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): see below
- Concentration of vehicle in final test solution:

Test substance[µg/L] Dispersant[µg/L]
_______________________________________________
Tween-80 DMSO
_______________________
15 75 4130
1.5 7.5 413
_______________________________________________

Test organisms

Test organisms (species):
Cyprinus carpio
Details on test organisms:
TEST ORGANISM
- Common name: carp
- Source: Sugishima fish farm, Kumamoto
- Length at study initiation (lenght definition, mean, range and SD): mean 10.6 cm
- Weight at study initiation (mean and range, SD): mean 32 g
- Lipid content: 5 %
- Feeding during test (no diet on day of sampling):
- Food type: pelleted feed for carp, Japan Haigo Shiryo K.K.
- Amount: corresponding to 2 % of bw
- Frequency: 2x/d


ACCLIMATION
- Acclimation period: 1 - 2 months after external disinfection
- Disinfection: for about 24 h under static condition using 50 mg/L Terramycin and 7 g/L NaCl
- Acclimation conditions (same as test or not): flow-through, 25 +-2°C
- Health during acclimation (any mortality observed): abnormal fish removed

Study design

Route of exposure:
aqueous
Test type:
flow-through
Water / sediment media type:
natural water: freshwater
Total exposure / uptake duration:
8 wk

Test conditions

Test temperature:
25 +-2°C
Dissolved oxygen:
6 - 8 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: glass tank, 100 L test volume
- Type of flow-through (e.g. peristaltic or proportional diluter):
- Renewal rate of test solution (frequency/flow rate): 1155 L/d = approx. 0.8 L/min
- No. of organisms per vessel: 8 - 10 fish per test concentration
- No. of vessels per concentration (replicates): 1
- Biomass loading rate: =< 3 g/L (initial)
- Test period: 8 weeks


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: underground water


OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: no data


Nominal and measured concentrations:
1.5 and 15 µg/L
Reference substance (positive control):
no
Details on estimation of bioconcentration:
BASIS FOR CALCULATION OF BCF

BCF = conc(total fish tissue, wet) / conc(water)

Results and discussion

Bioaccumulation factoropen allclose all
Type:
BCF
Value:
2 615
Basis:
whole body w.w.
Remarks:
average of two samples
Time of plateau:
6 wk
Calculation basis:
steady state
Remarks on result:
other: Conc.in environment / dose:15 µg/L
Type:
BCF
Value:
2 105
Basis:
whole body w.w.
Remarks:
average of two samples
Time of plateau:
8 wk
Calculation basis:
steady state
Remarks on result:
other: Conc.in environment / dose:15 µg/L
Type:
BCF
Value:
1 990
Basis:
whole body w.w.
Remarks:
one sample
Time of plateau:
6 wk
Calculation basis:
steady state
Remarks on result:
other: Conc.in environment / dose:1.5 µg/L
Type:
BCF
Value:
1 790
Basis:
whole body w.w.
Remarks:
average of two samples
Time of plateau:
8 wk
Calculation basis:
steady state
Remarks on result:
other: Conc.in environment / dose:1.5 µg/L
Depuration
Elimination:
not specified
Details on results:
- Mortality of test organisms: none
- Behavioural abnormalities: none
- Other biological observations: none

Any other information on results incl. tables

Measured concentration in test water [µg/L]:

Level [µg/L]

2w

4w

6w

8w

15

14.8

15.6

15.4

15.4

1.5

1.56

1.53

1.50

1.51

Bioconcentration factor (based on measured concentration):

Level [µ/L]

2w

4w

6w

8w

15

1840

1920

2410

2750

2060

2340

2820

1660

Mean

1950

2130

2615

2105

1.5

2110

1790

1990

1880

2020

2710

903

1700

Mean

2065

2250

?

1790

Applicant's summary and conclusion