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Ecotoxicological information

Long-term toxicity to aquatic invertebrates

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Administrative data

Endpoint:
long-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline study, meets national standard methods, but significant deficiencies (dose selection: highest non-effective test concentration was significantly below the maximum water solubility)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2000
Report Date:
2000

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
other: AFNOR (draft, not further specified)
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): anthracene
- Molecular formula (if other than submission substance): C14H10
- Molecular weight (if other than submission substance): 178
- Substance type: organic
- Physical state: solid
- Source: no data
- Analytical purity: no data
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
PHYSICO-CHEMICAL PROPERTIES (data from NL 2008: Coal-Tar Pitch, Annex-XV dossier, and WHO 1998: EHC 202)
- Water solubility: 50 - 70 µg/L (20 - 25 °C)
- Henry's law constant: 4.3 Pa*m3/mol (at 25 °C)
- log Pow: 4.3 - 4.5

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
no data

Test solutions

Vehicle:
yes
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Stock solution close to water saturation used for dilution series (no detailed data, see Report p. 34)
- Eluate: no
- Differential loading: no
- Controls: with and without vehicle
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): no data
- Concentration of vehicle in test medium (stock solution and final test solution(s) including control(s)): no data

Test organisms

Test organisms (species):
Ceriodaphnia dubia
Details on test organisms:
TEST ORGANISM
- Source: INERIS
- Age of parental stock (mean and range, SD): <24 h
- Feeding during test
- Food type: Chlorella vulgaris / Pseudokirchneriella subcapitata / fish diet
- Offspring: Males were counted and removed from the test medium.

Study design

Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
7 d

Test conditions

Hardness:
240 mg/L (as CaCO3)
Test temperature:
25 +- 2°C
pH:
8.1 +-0.4
Dissolved oxygen:
>7.0 mg/L
Nominal and measured concentrations:
Nominal and analytical values not documented.
Analytical: calculation of the geometric mean from measured concentrations in fresh and spent medium,
based on an arithmetic model (OECD 1999) (see Report, p. 44/45).
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): closed (screw cap)
- Material, size, headspace, fill volume: glass vessel (90 mL), test volume 50 mL
- Aeration: no
- Type of flow-through (e.g. peristaltic or proportional diluter):
- Renewal rate of test solution (frequency/flow rate): 1x/d, 5x/wk (each workday)
- No. of organisms per vessel: 1
- No. of vessels per concentration (replicates): 10
- No. of vessels per control (replicates): 10
- No. of vessels per vehicle control (replicates): none


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: natural mineral water (Evian)
- Ca/Mg ratio: approx. 3:1
- Conductivity: 460 +-100 µs/cm


OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 h light / 8 h dark
- Light intensity: 500 lux


EFFECT PARAMETERS MEASURED (with observation intervals if applicable): mortality adults and neonates, abortions, reproduction


VEHICLE CONTROL PERFORMED: yes

RANGE-FINDING STUDY
- Test concentrations: no data
Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Duration:
7 d
Dose descriptor:
EC10
Effect conc.:
> 3.4 µg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
dissolved
Remarks:
test material
Basis for effect:
other: mortality and reproduction
Remarks on result:
other: Highest test concentration was significantly below max. water solubility!
Duration:
7 d
Dose descriptor:
EC10
Effect conc.:
> 4.9 µg/L
Nominal / measured:
nominal
Conc. based on:
dissolved
Remarks:
test material
Basis for effect:
other: mortality and reproduction
Remarks on result:
other: Highest test concentration was significantly below max. water solubility!

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
Highest test concentration was significantly below max. water solubility, hence not fully evaluable.