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Zinc bis dimethyldithiocarbamate is highly toxic to aquatic organisms. Both acute and chronic exposure caused lethal and/or sublethal effects at all trophic levels tested in the laboratory (fish, aquatic invertebrates, algae, aquatic plants and microorganisms). Fish species showed the highest sensitivity to this substance among the short-term studies, whereas aquatic invertebrates were found to be more sensitive under chronic exposure conditions (LC50 = 0.0097 mg a.i./L and NOEC = 78 µg a.i./L respectively). This substance caused immobilisation and reproduction-related problems in aquatic invertebrates and growth inhibition in algae (EC50 = 0.5 mg a.i./L) . Furthermore, inhibitory effects were observed in aquatic microorganisms’ respiration as well (EC50 = 19.2 mg/L).