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EC number: 205-288-3 | CAS number: 137-30-4
- Life Cycle description
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- Appearance / physical state / colour
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- Density
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- Endpoint summary
- Stability
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- Environmental data
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- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
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- Toxicological Summary
- Toxicokinetics, metabolism and distribution
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Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 15 February 1999 - 17 May 1999
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method:
Test substance: a stock dispersion of the test substance was prepared by dispering 0.2010 g of Ziram in 1000 mL of BOD dilution water
Reference substance: a stock solution of the reference substance was prepared according to the OECD guideline by dissolving 0.1251 g in 5 mL NaOH solution, adjusting the pH to 7.7 with HCl solution, and the making up to 250 mL with Nanopure water
- Controls: yes - Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Source: oxidation ditch treating domestic sewage, Berkel en Rodenrijs, The Netherlands
- Preparation of inoculum for exposure: the sample was centrifugated and the supernatant discarded. Activated sludge was washed three times by centrifugation (10 min at 2000 rpm = max. 1218 g) and resuspension in tap water twice and in dilution water once. The dry weight of the sludge suspension was determined and the suspension diluted to approximately 4 g/L of mixed liquor suspended solids (MLSS) by addition of dilution water. Then, it was aerated vigorously and kept at approximately 20°C in the dark. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Test temperature:
- 20°C
- pH:
- 7.9
- Nominal and measured concentrations:
- Nominal concentrations: 0.10, 0.32, 1, 3.2, 10, 32 and 100 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 1 L glass beakers containing 16 mL of synthetic sewage feed, 284 mL of control (dilution water) or treatment solutions and 200 mL of activated sludge
- Aeration: 0.5 to 1 L/min
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 2
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: the dilution water was prepared according to NEN standard No. 6634.
- Synthetic sewage feed was prepared according to OECD guideline
EFFECT PARAMETERS MEASURED: respiration inhibition
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 3.2 - Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 19.2 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.32 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Results with reference substance (positive control):
- The EC50 for 3,5-dichlorophenol was determined to be 13.2 mg/L, value within the validity range of the OECD guideline (5 to 30 mg/L)
- Validity criteria fulfilled:
- yes
Reference
Description of key information
EC50 (3 h) = 19.2 mg/L (nominal, activated sludge, OECD 209)
Key value for chemical safety assessment
- EC50 for microorganisms:
- 19.2 mg/L
Additional information
The effect of zinc bis dimethyldithiocarbamate (CAS No. 137-30-4) on aquatic microorganisms activity was investigated by Hanstveit et al. (1999). This test was conducted according to OECD Guideline 209, Activated Sludge, Respiration Inhibition Test (1984), under GLP conditions. After 3 hours of exposure to the test substance at 20°C, respiration rate inhibition in activated sludge microorganisms was reported, resulting in a EC50 (3 h) value of 19.2 mg/L.
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