Ziram

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1989

Report Date:

1989

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to

Guideline:

EPA OPP 81-2 (Acute Dermal Toxicity)

Test guideline 2

Qualifier:

equivalent or similar to

Guideline:

OECD Guideline 402 (Acute Dermal Toxicity)

Deviations:

yes

Remarks:

24h exposure

GLP compliance:

yes

Test type:

standard acute method

Limit test:

yes

Test material

Reference

Name:

Unnamed

Type:

Constituent

Type:

Constituent

Details on test material:

- Name of test material (as cited in study report): Ziram
- Lot no.: 8331 AA
- Purity: 98.5%

Test animals

Species:

rabbit

Strain:

New Zealand White

Sex:

male/female

Details on test animals and environmental conditions:

- Source: A. Smith, Warlingham, Surrey and Froxfield Rabbits, England
- Age at study initiation: 9-13 weeks
- Weight at study initiation: 2.2-2.9 kg

Administration / exposure

Type of coverage:

occlusive

Vehicle:

unchanged (no vehicle)

Duration of exposure:

24h

Doses:

2 g/kg

No. of animals per sex per dose:

5

Control animals:

not required

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 2 000 mg/kg bw

Mortality:

No deaths occured.

Clinical signs:

There were no signs of toxicity.

Body weight:

A loss in bodyweight was recorded for two females on Day 8. All other rabbits achieved anticipated bodyweight gains throughout the study.

Gross pathology:

Pale renal cortices were observed in the kidneys of one male at post-mortem. Terminal autopsy revealed no other macroscopic abnormalities.

Other findings:

Dermal responses:
Slight erythema only was observed in two males and two females. The reactions had resolved completely by Day 3 or 4 of the study. The remaining three males and three females showed no response to treatment.