(2S-cis)-2,3-dihydro-3-hydroxy-2-(4-methoxyphenyl)-1,5-benzothiazepin-4(5H)-one

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

December 1-3, 1998

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1999

Report date:

1999

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

(2S-cis)-2,3-dihydro-3-hydroxy-2-(4-methoxyphenyl)-1,5-benzothiazepin-4(5H)-one

EC Number:

255-799-0

EC Name:

(2S-cis)-2,3-dihydro-3-hydroxy-2-(4-methoxyphenyl)-1,5-benzothiazepin-4(5H)-one

Cas Number:

42399-49-5

Molecular formula:

C16H15NO3S

IUPAC Name:

(2S-cis)-2,3-dihydro-3-hydroxy-2-(4-methoxyphenyl)-1,5-benzothiazepin-4(5H)-one

Details on test material:

Description: white to slightly yellow powder
Storage conditions: room temperature

Sampling and analysis

Analytical monitoring:

not specified

Test solutions

Vehicle:

yes

Details on test solutions:

The test article solution was prepared by transferring suitable weighed amount of DTZ62 into glass beakers in order to obtain the final concentration of 10 mg/L.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

Number: 20 animals
Light: 12-16 hours of light/dark daily. During the test the animals were kept in the dark.
Feeding: the animals were fed with green algae (Selenastrum capricornutum) three times a week. During the treatment period the animals were not fed.
Housing during the treatment: 20 animals/concentration in silylated glass beakers. Vessel identification gave experimental number and group number.

Study design

Test type:

not specified

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Test conditions

Hardness:

251 mg/l

Test temperature:

Conc. 0 mg/L:
- 0 hour: 20
- 48 hours: 20
Conc 100 mg/L:
- 0 hour:20
- 48 hours: 20

pH:

Conc. 0 mg/L:
- 0 hour: 7.8
- 48 hours: 7.5
Conc 100 mg/L:
- 0 hour: 7.5
- 48 hours: 7.6

Dissolved oxygen:

Conc. 0 mg/L:
- 0 hour: 8.8 mg/L
- 48 hours: 8.7 mg/L
Conc 100 mg/L:
- 0 hour: 8.9 mg/L
- 48 hours: 8.2 mg/L

Details on test conditions:

Test description
Experimental design: on the basis of preliminary results, the test article was tested at nominal concentration of 100 mg/L.
One group of 20 animals was prepared as control group and treated with reconstituted water.
Observation of immobilization: at 24 and 48 hours. Daphnia were considered immobile if not able to swin within 15 seconds of gentle agitation of the test container.
Parameter checking: at 0 and 48 hours of treatment the following parameters were checked in the treatment solutions.

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Any other information on results incl. tables

Experimental data from an acute immobilization test in which Daphnia magna were treated with the test article DTZ 62 at the nominal concentration of 100 mg/ml are given in this report.

The animals were treated and observed for 48 hours.

No animals were immobilized in the control group.

One animal of the treated group was found immobilized at the 48h observation.

Among the other animals at 48 hours4 of them swan normally, while the others moved jerkily on the bottom of the test vessels with long periods of immobility.

The EC50 of DTZ 62 at 24 h and 48h was higher than 100 mg/L.

Applicant's summary and conclusion

Conclusions:

The EC50 of DTZ 62 at 24 h and 48h was higher than 100 mg/L.