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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1999
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1999
Report date:
1999

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
ISO 6341 (Water quality - Determination of the Inhibition of the Mobility of Daphnia magna Straus (Cladocera, Crustacea))
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Qualifier:
according to guideline
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
Salbutamol
EC Number:
242-424-0
EC Name:
Salbutamol
Cas Number:
18559-94-9
Molecular formula:
C13H21NO3
IUPAC Name:
4-[2-(tert-butylamino)-1-hydroxyethyl]-2-(hydroxymethyl)phenol
Test material form:
solid: particulate/powder

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Daphnia magna

Study design

Test type:
static
Water media type:
other: Medium M7
Limit test:
yes
Total exposure duration:
48 h

Test conditions

Hardness:
250 mg/l expressed as CaCO3
Test temperature:
21.0 C
pH:
pH 8.0 +/- 0.2 after aeration
Nominal and measured concentrations:
0.1, 1.0, 10 and 100 mg/l of Salbutamol Base.
Reference substance (positive control):
yes
Remarks:
Potassium dichromate

Results and discussion

Effect concentrations
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Conc. based on:
test mat.
Basis for effect:
mobility

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
Under the conditions of the present test, Salbutamol Base induced no effects in Daphnia magna exposed to a concentration of 100 mg/l. Hence, the 48h-EC50 for mobility of Daphnia magna was above 100 mg/l, the maximum concentration to be tested.
Executive summary:

A limit test was combined with a range-finding test exposing daphnids to Salbutamol Base concentrations of 0.1, 1.0, 10 and 100 mg/l. The 100 mg/l test group consisted of two vessels containing 10 daphnids per vessel. The 0.1,1.0 and 10 mg/l test groups consisted of one vessel containing 10 daphnids.

The study did not induce sampling for determination of actual exposure concentrations.

Salbutamol Base induced no effects in Daphnia magna exposed to a concentration of 100 mg/l. Hence, the 48h-EC50 for mobility of Daphnia magna was above 100 mg/l, the maximum concentration to be tested.