3,3'-[ethylenebis[(3-sulpho-p-phenylene)azo]]bis[5-amino-4-hydroxynaphthalene-2,7-disulphonic acid] hexasodium salt AND 3,3'-[ethylenebis[(3-sulpho-p-phenylene)azo]]bis[5-amino-4-hydroxynaphthalene-2,7-disulphonic] acid, compound with 2,2',2''-nitrilotriethanol (1:6)

Endpoint summary

Description of key information

Test on three trophic levels are available. The LC/EC50 for all tests was > 100 mg/L

Ten rainbow trouts/concentration were exposed to a formulation of the test substance at 0, 500 and 1000 mg/L. No mortality or toxic effects were observed at any of the concentrations tested. Measured concentrations were within 80 -120% of nominal. It can be concluded that the LC50 of the active ingredient is >100 mg/L (BASF 1987).

In a test according to OECD 202 daphnids were exposed to 0 and 100 mg/L during 48 hours in a static test. No mortality or effects on mobility were found. The measured test concentrations were 98 -99% of nominal. The EC50 is > 100 mg/L RCC 2005).

Lemna minor was exposed to an aqueous solution of the substance at concentrations of 1.0, 3.2, 10, 32 and 100 mg/L (three replicate flasks per concentration) for a period of 7 days (semistatic with renewal on day 2 and 4), under constant illumination at a temperature of  24 ± 1 °C.  The number of fronds in each control and treatment group was recorded on days 0, 3, 5 and 7 along with observations on plant development. Measured concentrations were 97 -111% of nominal. No effects on yield and growth rate were observed at any of the concentrations tested based on frond count and/or dry weight. The EC50 is > 100 mg/L (Envigo 2017).

In a limit test according to OECD 209, 1000 mg/L of the substance did not lead to inhibition of the respiratory activity of activated sludge. The NOEC is ≥ 1000 mg/L (RCC 2005)

Additional information

The starting point for the derivation of the PNECwater will be 100 mg/L. No sensitivity difference between the species tested was observed.

The starting point for the derivation of the PNEC STP will be 1000 mg/L