Reaction products of diazotised 2-amin-6-nitrophenol-4-sulphonic Acid and 2-Amino-4-nitrophenol-6-sulfonic Acid, coupled with a mixture of 6-Amino-4-hydroxy-2-naphthalenesulfonic Acid and Acetylated 6-Amino-4-hydroxy-2-naphthalenesulfonic Acid, subsequently metallized with Chromium (III) Basic Sulfate and Acetic Acid, and at the end diazotised and coupled with p-Cresol, sodium salts

Endpoint summary

Description of key information

Short-term toxicity to fish:

LC50 (Cyprinus carpio, 96 h) = 748 mg/l

Short-term toxicity to aquatic invertebrates:

EC50 (Daphnia magna, 24 h) = 216 mg/l

QSAR prediction: not classified for aquatic toxicity to Daphnia

Toxicity to aquatic plants other than algae:

ErC50 (Lemna minor, 7d) > 100 mg/l

Toxicity to microorganisms:

IC 50 (3 h)> 100 mg/l

Additional information

Adequate chronic toxicity data are not available for the substance; therefore the aquatic toxicity assessment and classification are based mainly on the short-term toxicity data available on the target substance.

Despite the substance has a log Kow very lower than 4 (indicating a low potential for bioaccumulation), it is not rapidly (bio)degradable.

According to the CLP Regulation (EC 1272/2008), acute aquatic toxicity is normally determined using a fish 96-hour LC50, a crustacean species 48-hour EC50 and/or an algal species 72- or 96-hour EC50.

The acute toxicity to fish, invertebrates and aquatic plants were conducted to:

-Cyprinus carpio according to the OECD Guideline 203 (1984);

-Daphnia magna according to the OECD Guideline 202 (1984);

-Lemna minor, according to the OECD Guideline 221 (2006).

According to the CLP Regulation (EC 12720/2008), the results obtained for the substance did not respect the classification criteria for the hazardous categories to the aquatic environment. Thus, the substance is not classified.