Trimethyl[2-(phosphonooxy)ethyl]ammonium chloride, calcium salt (1:1)

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

June 23, 1976 - August 27, 1976

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))

Deviations:

no

GLP compliance:

not specified

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge (adaptation not specified)

Details on inoculum:

The sludge was collected at 10 different sampling sites of Japan and mixed:
- industrial sludge (1x)
- municipal sludge (3x)
- surface water (3x river samples)
- surface soil (3x soil samples)

Duration of test (contact time):

14 d

Initial conc.:

100 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation:

O2 consumption

Parameter followed for biodegradation estimation:

TOC removal

Parameter followed for biodegradation estimation:

test mat. analysis

Remarks:

UV-VIS

Details on study design:

TEST CONDITIONS
- Test temperature: 25 ºC ± 1ºC
- pH: 7 ± 1
- Aeration of dilution water: yes
- Suspended solids concentration: 30 mg/l
- Other: Pretreatment of the analytical sample:
For 40 ml of sample the following was added:
- 5 ml 2.5% aqueous sodium solution
- 5 ml of coloring agent. The coloring agent was prepared mixing for 20 minutes before use the following: 20 ml of sodium nitroprusside solution 10%, 20 ml potassium ferricyanide aqueous solution 10% and 10 ml of sodium hydroxide aqueous solution.
The mixture was left for 15 minutes before filtration (sludge)
Then fresh and old activated sludge were mixed:
- 5 L of the filtrate of a supernatant of an activated sludge in present use and 500 ml of the filtrate of newly collected sludge
- cultivation at pH 7.0 +/- 1.0 and aeration
- addition of 0.1 (w/v)% synthetic sewage (consisting of a solution of glucose, peptone and monopotassium phosphate)
- Incubation for 14 days at 25ºC ± 1ºC

TEST SYSTEM
- Number of culture flasks/concentration: 1 vessel test item and 1 vessel positive reference
- Measuring equipment: UV-visible self recording spectrophotometer
- Other: Test performed in 300 ml vessels

CONTROL AND BLANK SYSTEM
- 1 vessel: water + test substance
- 1 vessel: control blank

Reference substance:

aniline

Key result

Parameter:

% degradation (O2 consumption)

Value:

93.5

Sampling time:

14 d

Parameter:

% degradation (test mat. analysis)

Value:

100

Sampling time:

14 d

Results with reference substance:

Aniline: 40% and 60% biodegradation (by BOD) after 7 and 14 days respectively

Validity criteria fulfilled:

yes

Remarks:

percentage biodegradation of aniline (by BOD) were beyond 40% and 60% after 7 and 14 days respectively

Interpretation of results:

readily biodegradable

Conclusions:

The test item was shown to be readily biodegradable (93.5% of biodegradation within 14 days).

Executive summary:

A study of ready biodegradability of test item was performed according to OECD 301C. Test solutions containing test item (100 mg/l) or reference substance (aniline) were inoculated with activated sludge (30 mg/L SS) and incubated under aerobic conditions at constant temperature (25 ± 1ºC) for 14 days. Control solutions were also run in parallel. Oxygen consumption was analyzed was monitored. Reference substance showed 40% and 60% biodegradation (by BOD) after 7 and 14 days respectively, validating the assay. At the 14th day of the test, the measured aerobic biodegradation of the test item was 93.5%. Thus, the test item can be deemed to be readily biodegradable.

Description of key information

The available data refer to the degradation product of calcium phosphorylcholine chloride:

- Key study. Method according OECD 301C. The substance choline chloride was shown to be readily biodegradable (93.5% of biodegradation within 14 days).

Based on this information, it can be concluded that the substance phosphoryl choline chloride is readily biodegradable.

Key value for chemical safety assessment

Biodegradation in water:

readily biodegradable

Additional information

- Key study: A study of ready biodegradability of test item was performed according to OECD 301C. Test solutions containing choline chloride (100 mg/l) or reference substance (aniline) were inoculated with activated sludge (30 mg/L SS) and incubated under aerobic conditions at constant temperature (25 ± 1ºC) for 14 days. Control solutions were also run in parallel. Oxygen consumption was analysed was monitored. Reference substance showed 40% and 60% biodegradation (by BOD) after 7 and 14 days respectively, validating the assay. At the 14th day of the test the measured aerobic biodegradation of the test item was 93.5%. Thus, the test item can be deemed to be readily biodegradable.