Trimethyl[2-(phosphonooxy)ethyl]ammonium chloride, calcium salt (1:1)

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Study period:

June 23, 1976 - August 27, 1976

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

other: OECD Guideline 301 (Ready Biodegradability. Modified MITI Test (1))

Deviations:

no

GLP compliance:

no

Analytical monitoring:

yes

Vehicle:

not specified

Test organisms (species):

activated sludge

Details on inoculum:

The sludge was collected at 10 different sampling sites of Japan and mixed:
- industrial sludge (1x)
- municipal sludge (3x)
- surface water (3x river samples)
- surface soil (3x soil samples)

Test type:

static

Limit test:

yes

Total exposure duration:

14 d

Test temperature:

25 ºC ± 1 ºC

pH:

pH: 7 ± 1

Nominal and measured concentrations:

100 mg/L

Details on test conditions:

TEST SYSTEM
- Material, size, headspace, fill volume: 300 mL
- Aeration: yes
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Sludge concentration (weight of dry solids per volume): 30 mg/L

OTHER TEST CONDITIONS
- Pretreatment of the analytical sample:
For 40 ml of sample the following was added:
- 5 ml 2.5% aqueous sodium solution
- 5 ml of coloring agent. The coloring agent was prepared mixing for 20 minutes before use the following: 20 ml of sodium nitroprusside solution 10%, 20 ml potassium ferricyanide aqueous solution 10% and 10 ml of sodium hydroxide aqueous solution.
The mixture was left for 15 minutes before filtration (sludge)
Then fresh and old activated sludge were mixed:
- 5 L of the filtrate of a supernatant of an activated sludge in present use and 500 ml of the filtrate of newly collected sludge
- cultivation at pH 7.0 +/- 1.0 and aeration
- addition of 0.1 (w/v)% synthetic sewage (consisting of a solution of glucose, peptone and monopotassium phosphate)
- Incubation for 14 days at 25ºC ± 1ºC

TEST CONCENTRATIONS
- Test concentrations: 100 mg/L

Reference substance (positive control):

yes

Remarks:

Aniline

Key result

Duration:

14 d

Dose descriptor:

NOEC

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: % biodegradability

Details on results:

Test item Choline chloride was readily biodegradable under test conditions.

Results with reference substance (positive control):

- Results with reference substance valid? Yes.
Aniline: 40% and 60% biodegradation (by BOD) after 7 and 14 respectively.

Validity criteria fulfilled:

yes

Remarks:

percentage biodegradation of aniline (by BOD) were beyond 40% and 60% after 7 and 14 days respectively.

Conclusions:

The NOEC for microorganism toxicity of the test item was determined to be 100 mg/L.

Executive summary:

A ready biodegradation test was performed on Choline chloride according to OECD Guideline 301 C (Modified MITI Test I). 100 mg/L of activated sludge were exposed to 100 mg/L test item for 14 days. The biodegradation obtained was 93.5% (based on BOD), therefore the test item was determined to be readily biodegradable. According to the current guidelines, the NOEC for microorganism toxicity was determined to be 100 mg/L since the compound degrades well in the ready biodegradability study.

Description of key information

The available data refer to the degradation product of calcium phosphorylcholine chloride:

- Key study. Method according OECD 301. The test item was not inhibitory to microorganisms (93.5% biodegradation in 14d) at a concentration of 100 mg/L. Therefore, the NOEC value for the test item in microorganisms is greater than 100 mg/L.

Based on this information, it can be concluded that the substance phosphoryl choline chloride has a NOEC for microorganism toxicity of 100 mg/L.

Key value for chemical safety assessment

EC10 or NOEC for microorganisms:

100 mg/L

Additional information

-  Key study. A ready biodegradation test was performed on choline chloride according to OECD Guideline 301 (Modified MITI Test I). 100 mg/L of activated sludge were exposed to 100 mg/L test item for 14 days. The biodegradation obtained was 93.5% (based on BOD), therefore the test item was determined to be readily biodegradable. According to the current guidelines, the NOEC for microorganism toxicity was determined to be 100 mg/L since the compound degrades well in the ready biodegradability study.