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Short-term toxicity to aquatic invertebrates

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short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
06 - 27 Jul 2009
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions
according to guideline
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Temperature much higher than recommended by the guideline.
according to guideline
EU Method C.2 (Acute Toxicity for Daphnia)
GLP compliance:
yes (incl. QA statement)
Landesamt für Umwelt, Wasserwirtschaft und Gewerbeaufsicht, Mainz, Germany
Analytical monitoring:
Details on sampling:
- Concentrations: 0 and 100 mg/L
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: the solubility of the test item lies below 100 mg/L, thus in a second test the water accommodated fraction was prepared for the test. The nominal load was weighed, adding the corresponding amount of dilution water and shaking vigorously for 24 h. The solution was then filtered through 0.45 µm filter.
Test organisms (species):
Daphnia magna
Details on test organisms:
- Common name: water flea
- Strain: Berlin
- Source: Umweltbundesamt, Berlin, Germany
- Age at study initiation (mean and range, SD): between 0 - 24 h
- Method of breeding: Daphnids were bred in the laboratory (similar to the method EN ISO 6341 following SOP 11500201) throughout the year for the use in toxicity tests. They multiply by parthenogenesis.
- Feeding during breeding: unicellular green algae (Desmodesmus subspicatus)
- Medium: M4-Medium (recipe of ELENDT)
- Temperature during breeding: 20 ± 2 °C
- Photoperiod: 16/8 h using neon tubes

Test type:
Water media type:
Limit test:
Total exposure duration:
48 h
Test temperature:
26.2 - 26.8 °C (test item)
24.4 - 25 (positive control)
7.6 - 7.8 (test item)
7.5 - 7.7 (positive control)
Dissolved oxygen:
7.6 - 7.7 mg/L (test item)
7.9 - 8.6 mg/L (positive control)
Nominal and measured concentrations:
100 mg/L (nominal)
1.2 mg/L (mean measured)
Details on test conditions:
- Fill volume: 20 mg/L
- Aeration: yes
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

- Source/preparation of dilution water: according to guideline
- Culture medium different from test medium: yes
- Intervals of water quality measurement: the content of the test item, pH and concentration of dissolved oxygen were measured at the beginning and at the end of the test

- Adjustment of pH: yes, to 7.8 ± 0.2

EFFECT PARAMETERS MEASURED: after 24 and 48 h the immobilised Daphnids were counted

- Range finding study: yes, without analytics, not further data in the report
Reference substance (positive control):
Potassium dichromate
48 h
Dose descriptor:
Effect conc.:
> 1.2 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
Remarks on result:
other: corresponding to 100 mg/L nominal concentration
Details on results:
- Mortality of control: no
- Other biological observations: the concentration tested showed no toxicity to Daphnids
Results with reference substance (positive control):
- EC50 (24 h): 0.7 mg/L (95% CI: 0.6 - 1.7 mg/L)
- EC100 (24 h): 2 mg/L
- NOEC (24 h): 0.5 mg/L

Table 1: Effect concentrations.

Parameter Value
24 h 48 h
EC50 > 1.2 mg/L > 1.2 mg/L
EC100 > 1.2 mg/L > 1.2 mg/L
NOEC ≥1.2 mg/L ≥1.2 mg/L

* 95% CI was not determinable

Table 2: Measured concentrations.

Nominal concentr. in mg/L Measured concentr. in mg/L % recovery after 48 h % of nominal concentr. Mean meas. concentr. in mg/L
0 h 48 h 0 h  48 h
0 < LOQ < LOQ  -  -  -  -
100 1.2 1.1 90 1 1 1.2

LOQ = 0.08 mg/L

Because of unexpected low measured concentrations an additional experiment was performed and the results are shown in table 3.

Table 3: Measured concentrations of the test item when prepared as WAF in both deionised and dilution water filtered and unfiltered.

Nominal concentr. in mg/L Measured concentr. in mg/L in deionised water Measured concentr. in mg/L in dilution water
filtered unfiltered filtered unfiltered
100 4.9 5.1 2 1.8
Validity criteria fulfilled:

Description of key information

EC50 (48 h) > 1.2 mg/L (mean measured, corresponding to 100 mg/L nominal) for Daphnia magna (OECD 202)

Key value for chemical safety assessment

Additional information

One study is available investigating the short term toxicity of the substance (CAS 72869-86-4) to aquatic invertebrates. The study was conducted according to OECD 202 and GLP. A nominal load of 100 mg test item/L was weighed into the test vessel, adding the corresponding amount of dilution water and shaking vigorously for 24 h. The solution was then filtered through a 0.45 µm filter. The test organism Daphnia magna was then exposed in a static system for 48 h to the single nominal concentration (limit test). An analytical monitoring was performed via HPLC-UV-VIS and 1.2 mg test item/L (mean) was measured. The EC50 (48 h) was determined to be 1.2 > mg/L (mean measured).