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Ecotoxicological information

Short-term toxicity to fish

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Administrative data

short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
23.12. - 27.12.1980
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference Type:
Acute toxicities of organic chemicals to Fathead minnows (Pimephales promelas)
Brooke, L.T., Call, D.J., Geiger, D.L. & Northcott, C.E.
Bibliographic source:
ISBN 0-9614968-0-0

Materials and methods

Test guideline
equivalent or similar to guideline
OECD Guideline 203 (Fish, Acute Toxicity Test)
not applicable
GLP compliance:

Test material

Constituent 1
Chemical structure
Reference substance name:
EC Number:
EC Name:
Cas Number:
Molecular formula:
Details on test material:
Chemical Source: Aldrich Chemical Co, Milwaukee, USA
Purity: 97 %
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
no data available

Sampling and analysis

Analytical monitoring:
Details on sampling:
All test exposure chambers were sampled at approximately mid-depth at 0 and 96 hr and one of each duplicate exposure chamber at 24, 48 and 72 hr

Test solutions

not specified
Details on test solutions:
no data available

Test organisms

Test organisms (species):
Pimephales promelas
Details on test organisms:
Fathead minnows used in the tests were cultured from brood stock provided by the U.S. ERA Environmental Research Laboratory-Duluth. Adults were held at 25 C in flowing water with a 16 hr light-controlled photoperiod and fed frozen adult brine shrimp (Artemia sp.).
Age at test start: 28-31 days, mean length: 21.1 mm, mean weight: 0.172 g

Study design

Test type:
Water media type:
Limit test:
Total exposure duration:
96 h
Post exposure observation period:
no data available

Test conditions

45.4 mg/L CaCO3
Test temperature:
24.0 °C
Dissolved oxygen:
6.9 mg/L
39.5 as CaCO3
Nominal and measured concentrations:
nominal: 0, 2.6, 4.3, 7.2, 12.0, 20.0
analysed: 0, 0.73, 1.4, 2.7, 5.5, 12.3
Details on test conditions:
Exposures were conducted in two-L battery jars. Test solutions were renewed by siphoning 90% of the solutions from the jars and replacing them with fresh stock of the appropriate concentration. The exchange of solutions was done at least every 48 hr.
Fish were not fed during chemical exposures. During exposure to toxicants the fish were routinely observed for behavioral responses (effects) and deaths.
Reference substance (positive control):
not specified

Results and discussion

Effect concentrationsopen allclose all
96 h
Dose descriptor:
Effect conc.:
2.2 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Remarks on result:
other: 95% CL: 2.0-2.4 mg/L
96 h
Dose descriptor:
Effect conc.:
2.2 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
Remarks on result:
other: 95% CL: 2.0-2.4 mg/L
Details on results:
affected fish stopped schooling, swam at the water surface and lost equilibrium prior to death.
Results with reference substance (positive control):
not applicable
Reported statistics and error estimates:
Observations of affected and dead fish exposed to test item were combined when duplicate exposures were made, resulting in a single LC50 a estimate per test. Estimation of the LC50 was made using the "trimmed Spearman-Karber" method (Hamilton et_ al_., 1977).

Applicant's summary and conclusion

Based on the test results, the 96-hour LC50 for fathead minnow (Pimephales promelas) for 4-chlorobenzaldehyde was determined to be 2.2 mg/L based on measured concentrations.
Executive summary:

The acute toxicity of 4-chlorobenzaldehyde to fathead minnow (Pimephales promelas), was determined in an aerated, semi static, 96-hour test. Treatments consisted of a dilution water control and the nominal concentrations of 2.6, 4.3, 7.2, 12 and 20 mg/L.

Based on the test results, the 96-hour LC50for fathead minnow (Pimephales promelas

for 4-chlorobenzaldehyde was determined to be 2.2 mg/L based on measured concentrations.