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Diss Factsheets

Administrative data

Endpoint:
acute toxicity: inhalation
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1982
Report date:
1982

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 403 (Acute Inhalation Toxicity)
Deviations:
not applicable
GLP compliance:
no
Test type:
standard acute method
Limit test:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
4-chlorobenzaldehyde
EC Number:
203-247-4
EC Name:
4-chlorobenzaldehyde
Cas Number:
104-88-1
Molecular formula:
C7H5ClO
IUPAC Name:
4-chlorobenzaldehyde
Details on test material:
- Name of test material (as cited in study report): 4-Chlorobenzaldehyde
- Physical state: crystalline
- Analytical purity: technically pure
- Lot/batch No.: 2593

Test animals

Species:
rat
Strain:
Wistar
Sex:
male/female
Details on test animals or test system and environmental conditions:
TEST ANIMALS
- Source: Breeder Winkelmann, Borchen, Germany
- Weight at study initiation: 160-200 g
- Housing: Makrolon cages typ III
- Diet ( ad libitum): "Altromin R-Haltungsdiät"
- Water (ad libitum): yes


ENVIRONMENTAL CONDITIONS
- Temperature (°C): yes
- Humidity (%): yes
- Air changes (per hr): yes
- Photoperiod (hrs dark / hrs light): yes

Administration / exposure

Route of administration:
inhalation: vapour
Type of inhalation exposure:
nose/head only
Vehicle:
other: unchnaged (no vehicle)
Details on inhalation exposure:
Rats were exposed to vapor, generated by bubbling 200 l/h air through 920 or 980 g of the substance.
Analytical verification of test atmosphere concentrations:
yes
Duration of exposure:
>= 4 - <= 7 h
Concentrations:
- 4 h inhalation: > 321 mg/m³ (saturated)
- 7 h inhalation: 250 mg/m³
No. of animals per sex per dose:
- 10 males and females per dose
Control animals:
no
Details on study design:
- The animals were observed for signs of toxicity and mortality over a period of 14 days.
- Necropsy of survivors performed: yes

Results and discussion

Effect levelsopen allclose all
Sex:
male/female
Dose descriptor:
LC50
Effect level:
> 321 mg/m³ air
Based on:
test mat.
Exp. duration:
3 h
Remarks on result:
other: saturated
Sex:
male/female
Dose descriptor:
LC50
Effect level:
> 250 mg/m³ air
Based on:
test mat.
Exp. duration:
7 h
Mortality:
No mortality was seen.
Clinical signs:
other: Apart from irritation of the mucose membranes of the eye and nose and unspecific behavioural disorder of the animals no findings were noted at necropsy.
Body weight:
The weight gain of the 14-day observation period was normal and physiological.
Gross pathology:
4h exposure group: no changes
7 h exposure group: no changes (males), sporadically dicolored livers (females)

Applicant's summary and conclusion

Executive summary:

In an acute inhalation toxicity study 10 male and female Wistar rats per group were exposed to 4 -Chlorobenzaldehyde for 3 or 7 hours at concentrations of 321 mg/m³ or 250 mg/m³, respectively. Animals then were observed over a period of 14 days.

No mortality was seen. Apart from irritation of the mucose membranes of the eye and the nose and unspecific behavioural disorder of the animals no findings were noted at necropsy. The weight gain of the 14-day observation period was normal and physiological.

The following LC 50 values were determined:

LC 50 3 h-Exposure: > 321 mg/m ³

LC 50 7 h-Exposure: > 250 mg/m³