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Environmental fate & pathways

Biodegradation in water: screening tests

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Description of key information

Not readily biodegradable

Key value for chemical safety assessment

Biodegradation in water:
under test conditions no biodegradation observed

Additional information

No information on the biodegradation in water of the Target Substance is available. However data on a Similar Substance has been taken into account for the assessment. More details on the substance similarity are reported in section 13.

Ready biodegradability

To evaluate the ready biodegradability of the test substance (80 % act. integr.), the biological oxygen demand (BOD) and the chemical oxygen demand (COD) were determined according to the EU method C.5 or EU method C.6, respectively. The BOD of the test substance was determined by measuring the dissolved oxygen concentration before and after 5 days incubation at 20 °C in different test concentrations (6 to 800 mg/L) diluted in water saturated with dissolved oxygen that contains seeding water of an urban waste water. The BOD5 was 4 mg O2/g. The BOD5 of the reference substance D(+)-Glucose/L-Glutamic acid, which was tested in a concentration of 20 ml/L was 205 mg O2/L.

Regarding the COD, 5.0 mg of the test susbtance (80% act. integr.) dissolved in water was oxidized by potassium dichromate in a strong sulphuric acid medium with silver sulphate as a catalyst under reflux for 2 hours. The residual dichromate was determined by titration with standardized ferrous ammonium sulphate. The COD value was 876 mg O2/g. The reference substance Potassium hydrogen phthalate was tested in a concentration of 20 ml/L. The COD was 201 mg O2/L. The BOD5/COD ratio was calculated to be < 1%.

inherent biodegradability

To assess the inherent biodegradability of the test substance in two different purities, a study was conducted according to the 28 days Zahn-Wellens test (OECD 302 B). 463 mg/L of the test substance (80 % act. integr.) was incubated with 930 mg/L sludge of a biological sewage treatment plant at 22 °C under indirect daylight. The DOC removal after 28 days corrected by the blank control was 1.7 %. For the reference susbtance Diethylenglycol the DOC removal was 99.4 %. Concerning the test substance (60 % act. integr.), 872 mg/L of the test substance was incubated with 1030 mg/L sludge. The DOC removal was found to be 6 %.

In conclusion based on these results, no biodegradation in water was observed under the chosen conditions.